脑力负荷是影响飞行安全的重要因素之一,对飞行员脑力负荷进行客观测量,对于飞机座舱显示界面脑力任务的工效评价与优化设计具有重要意义.基于ERP(Eveat Related Potential)技术,选取失匹配负波(MMN,Mismatch Negativity)和P3a成分为指...脑力负荷是影响飞行安全的重要因素之一,对飞行员脑力负荷进行客观测量,对于飞机座舱显示界面脑力任务的工效评价与优化设计具有重要意义.基于ERP(Eveat Related Potential)技术,选取失匹配负波(MMN,Mismatch Negativity)和P3a成分为指标,采用三刺激"oddball"模式,在飞行模拟任务条件下开展了三级脑力负荷的测量与评价研究.实验结果表明,MMN峰值和P3a峰值对脑力负荷变化敏感,随着脑力负荷的增加,MMN峰值显著增加,而P3a峰值显著降低,反应了被试者对异常信息的自动加工能力的提高以及朝向注意能力的减弱.与新异刺激相比,由偏差刺激所诱发的MMN与P3a成分对于与飞行任务相关的脑力负荷具有更好的敏感性,将可用于进一步的脑力负荷分级评价.展开更多
Objective： To observe the effect of norcantharidin（NCTD） on collagen-induced arthritis（CIA） rats. Methods： Sixty Sprague-Dawley（SD） rats were randomly divided into 6 groups（n=10）： normal group, CIA model gr...Objective： To observe the effect of norcantharidin（NCTD） on collagen-induced arthritis（CIA） rats. Methods： Sixty Sprague-Dawley（SD） rats were randomly divided into 6 groups（n=10）： normal group, CIA model group（model group）, NCTD low-dose group [1.35 mg/（kg·d）], NCTD middle-dose group [2.7 mg/（kg·d）], NCTD high-dose group [5.4 mg/（kg·d）] and methotrexate（MTX） group [1.8 mg/（kg/w）]. Anesthetized rats were sacrificed by luxation of cervical vertebra after 4 weeks of administration. The arthritis scores were evaluated twice a week. The pathological changes in the ankle joints of rats were observed by hematoxylin-eosin（H＆E） staining. The serum levels of interleukin（IL） 1β, IL-6, tumor necrosis factor（TNF）-α, vascular endothelial growth factor（VEGF）, IL-17 and transform growth factor（TGF） β were detected by enzyme linked immunosorbent assay（ELISA）. The mRNA expression of retinoid-related orphan nuclear receptor γ t（ROR γ t） and forkhead box P3（Foxp3） in peripheral blood lymphocytes were confirmed by real-time polymerase chain reaction. Results： MTX and high-dose NCTD not only decreased the arthritis scores but also alleviated the pathological changes in CIA rats＇ ankle joints compared with the model group（P〈0.05 or P〈0.01）. All doses of NCTD significantly inhibited the serum levels of IL-6, IL-17 and TNF-α in CIA rats（P〈0.05）. Only middle-and high-dose of NCTD prominently decreased serum IL-1β and TGF-β levels of CIA rats（P〈0.05）. However, NCTD has no effect on vascular endothelial growth factor（VEGF） level in CIA rats. The Foxp3 mRNA expression in all NCTD groups were increased significantly than in the model group（P〈0.05）. The mRNA expression of RORγt in NCTD high-dose group was decreased apparently in comparison with the model group（P〈0.05）. Conclusion： NCTD showed therapeutic effect on CIA rats by inhibition of cytokines and regulation of Th17/Treg cells.展开更多
Increasing evidence indicates a role for regulatory T cells(Tregs)in the immune response and in autoimmune diseases,but the role of Tregs and cytokines in autoimmune hepatic diseases remains largely unclear and contro...Increasing evidence indicates a role for regulatory T cells(Tregs)in the immune response and in autoimmune diseases,but the role of Tregs and cytokines in autoimmune hepatic diseases remains largely unclear and controversial,especially in patients with primary biliary cirrhosis(PBC).This study was undertaken to investigate Tregs and different cytokines in the liver and peripheral blood of PBC patients.We found that these patients demonstrated a reduction of CD4^(+)CD25^(+) T cells but elevated CD4^(+)Foxp3^(+) T cells in peripheral blood mononuclear cells(PBMCs)and CD41 T cells.The percentage of CD4^(+)CD25^(+) T cells in PBMCs was negatively correlated with elevated plasma interferon(IFN)-c levels.A liver-specific analysis showed that the frequency of Foxp31 Tregs,transforming growth factor(TGF)-b1 and IFN-c were increased in PBC patients.Our findings suggest that an imbalance between CD4^(+)CD25^(+) Tregs and cytotoxic cytokines plays a crucial role in the pathogenesis of PBC while the role of Foxp3 needs further investigation.展开更多
文摘脑力负荷是影响飞行安全的重要因素之一,对飞行员脑力负荷进行客观测量,对于飞机座舱显示界面脑力任务的工效评价与优化设计具有重要意义.基于ERP(Eveat Related Potential)技术,选取失匹配负波(MMN,Mismatch Negativity)和P3a成分为指标,采用三刺激"oddball"模式,在飞行模拟任务条件下开展了三级脑力负荷的测量与评价研究.实验结果表明,MMN峰值和P3a峰值对脑力负荷变化敏感,随着脑力负荷的增加,MMN峰值显著增加,而P3a峰值显著降低,反应了被试者对异常信息的自动加工能力的提高以及朝向注意能力的减弱.与新异刺激相比,由偏差刺激所诱发的MMN与P3a成分对于与飞行任务相关的脑力负荷具有更好的敏感性,将可用于进一步的脑力负荷分级评价.
文摘目的:探讨健脾化瘀解毒方通过抑制细胞焦亡防治胃癌前病变的作用机制。方法:构建胃癌前病变小鼠模型和细胞焦亡模型,将小鼠随机分为空白组,模型组,健脾化瘀解毒方高剂量组(15 g/kg)、低剂量组(7.5 g/kg)和维酶素组(0.2 g/kg),每组10只。分别进行干预后,观察小鼠胃黏膜组织病理变化及焦亡相关分子表达情况。细胞分为空白组、模型组和中药组,空白组与模型组给予空白血清,中药组给予含药血清干预后,除空白组外先后予以LPS、ATP处理。观察焦亡相关分子表达情况。结果:与空白组比较,模型组小鼠胃黏膜NLRP3、GSDMD、HMGB1表达显著升高(P<0.01),健脾化瘀解毒方高、低剂量组可显著降低其表达(P<0.01)。与空白组比较,模型组细胞焦亡关键分子NLRP3、GSDMD、Caspase-1 mRNA和蛋白表达水平升高(P<0.01,P<0.05),I L-1β、I L-18 m R NA表达水平显著升高(P<0.01),中药组可显著降低其表达水平(P<0.01,P<0.05)。结论:健脾化瘀解毒方可通过抑制细胞焦亡防治胃癌前病变。
基金Supported by the National Natural Science Foundation of China(No.81273837)
文摘Objective： To observe the effect of norcantharidin（NCTD） on collagen-induced arthritis（CIA） rats. Methods： Sixty Sprague-Dawley（SD） rats were randomly divided into 6 groups（n=10）： normal group, CIA model group（model group）, NCTD low-dose group [1.35 mg/（kg·d）], NCTD middle-dose group [2.7 mg/（kg·d）], NCTD high-dose group [5.4 mg/（kg·d）] and methotrexate（MTX） group [1.8 mg/（kg/w）]. Anesthetized rats were sacrificed by luxation of cervical vertebra after 4 weeks of administration. The arthritis scores were evaluated twice a week. The pathological changes in the ankle joints of rats were observed by hematoxylin-eosin（H＆E） staining. The serum levels of interleukin（IL） 1β, IL-6, tumor necrosis factor（TNF）-α, vascular endothelial growth factor（VEGF）, IL-17 and transform growth factor（TGF） β were detected by enzyme linked immunosorbent assay（ELISA）. The mRNA expression of retinoid-related orphan nuclear receptor γ t（ROR γ t） and forkhead box P3（Foxp3） in peripheral blood lymphocytes were confirmed by real-time polymerase chain reaction. Results： MTX and high-dose NCTD not only decreased the arthritis scores but also alleviated the pathological changes in CIA rats＇ ankle joints compared with the model group（P〈0.05 or P〈0.01）. All doses of NCTD significantly inhibited the serum levels of IL-6, IL-17 and TNF-α in CIA rats（P〈0.05）. Only middle-and high-dose of NCTD prominently decreased serum IL-1β and TGF-β levels of CIA rats（P〈0.05）. However, NCTD has no effect on vascular endothelial growth factor（VEGF） level in CIA rats. The Foxp3 mRNA expression in all NCTD groups were increased significantly than in the model group（P〈0.05）. The mRNA expression of RORγt in NCTD high-dose group was decreased apparently in comparison with the model group（P〈0.05）. Conclusion： NCTD showed therapeutic effect on CIA rats by inhibition of cytokines and regulation of Th17/Treg cells.
基金by a grant from the Jiangsu Province 135 Talent Foundation(RC2007004).
文摘Increasing evidence indicates a role for regulatory T cells(Tregs)in the immune response and in autoimmune diseases,but the role of Tregs and cytokines in autoimmune hepatic diseases remains largely unclear and controversial,especially in patients with primary biliary cirrhosis(PBC).This study was undertaken to investigate Tregs and different cytokines in the liver and peripheral blood of PBC patients.We found that these patients demonstrated a reduction of CD4^(+)CD25^(+) T cells but elevated CD4^(+)Foxp3^(+) T cells in peripheral blood mononuclear cells(PBMCs)and CD41 T cells.The percentage of CD4^(+)CD25^(+) T cells in PBMCs was negatively correlated with elevated plasma interferon(IFN)-c levels.A liver-specific analysis showed that the frequency of Foxp31 Tregs,transforming growth factor(TGF)-b1 and IFN-c were increased in PBC patients.Our findings suggest that an imbalance between CD4^(+)CD25^(+) Tregs and cytotoxic cytokines plays a crucial role in the pathogenesis of PBC while the role of Foxp3 needs further investigation.
