采用事件相关电位(Event-Related Potentials,E R P s)方法研究产品设计与性能的冲突对消费者决策过程的影响。在网络购物环境下,消费者对展示了设计与性能的产品进行购买决策,实验结束后根据设计(高审美吸引力/适中审美吸引力)与性能(...采用事件相关电位(Event-Related Potentials,E R P s)方法研究产品设计与性能的冲突对消费者决策过程的影响。在网络购物环境下,消费者对展示了设计与性能的产品进行购买决策,实验结束后根据设计(高审美吸引力/适中审美吸引力)与性能(高/低)是否一致将产品刺激划分为四种组别:组别1(一致:高审美吸引力设计+高性能)、组别2(冲突:高审美吸引力设计+低性能)、组别3(冲突:适中审美吸引力设计+高性能)和组别4(一致:适中审美吸引力设计+低性能)。研究结果显示,组别2和3会在大脑前额皮层诱发波幅较大的负成分N270波,说明消费者根据产品设计形成的性能期望与实际性能不符,形成了刺激与大脑内源信息的冲突,从神经学角度验证了"消费者认为高审美吸引力的设计意味着高性能"。组别1和2会在大脑顶区皮层诱发波幅较大的正成分P2波,这两个组别产品的确定购买率也较高,表明产品设计的美学对消费者的购买决策有正向影响。性能信息也影响着消费者对产品的审美体验,当产品设计的审美吸引力高时,冲突会降低消费者对产品的审美体验,进而降低产品的确定购买率;而当设计的审美吸引力适中时,冲突会提高消费者对产品的审美体验,进而提高产品的确定购买率。对比具有冲突的两个组别,组别2的产品确定购买率高于组别3,说明存在冲突时,审美体验也正向影响消费者的决策。该发现解释了消费者在网络购物时浏览商品照片和规格参数界面时的神经和心理层面的决策过程。展开更多
Background The CYP2C19 G681A single polymorphism has been proven to affect clopidogrel responsiveness. However, the effect of coexisting polymorphisms of other genes has not yet been reported in the Chinese population...Background The CYP2C19 G681A single polymorphism has been proven to affect clopidogrel responsiveness. However, the effect of coexisting polymorphisms of other genes has not yet been reported in the Chinese population. This study investigated the effect of coexisting polymorphisms of CYP2C19 and P2Y12 on clopidogrel responsiveness and adverse clinical events in Chinese patients. Methods In 577 Han Chinese patients undergoing stent placement because of acute coronary syndrome had platelet reactivity assessed by thromboelastography, and the CYP2C19 G681A and P2Y12 C34T polymorphisms were detected by the ligase detection reaction. Primary clinical endpoints included cardiovascular death, nonfatal myocardial infarction, target vessel revascularization, and stent thrombosis. The secondary clinical endpoints were thrombolysis in myocardial infarction bleeding. The follow-up period was 12 months. Results Genotyping revealed 194 carriers of the wild type GG genotype of CYP2C19 and the wild type CC genotype of P2Y12 （group 1）, 102 carriers of the wild type GG genotype of CYP2C19 and the mutational T allele of P2Y12 （group 2）, 163 carriers of the mutational A allele of CYP2C19 and the wild type CC genotype of P2Y12 （group 3）, and 118 carriers of the mutational A allele of CYP2C19 and the mutational T allele of P2Y12 （group 4）. Group 4 had the lowest ADP-inhibition （49.74＋32.61） and the highest prevalence of clopidogrel low response （29.7%） of the four groups. The rate of the composite of primary clinical endpoints increased more in group 4 （8.5%） than in the other three groups; the rate of composite primary endpoints in group 2 （2.9%） and group 3 （3.7%） were not significantly different than that of group 1 （1.5%）. Conclusion Coexisting polymorphisms of different genes affected clopidogrel responsiveness and clinical outcome more than single polymorphism in Chinese patients with acute coronary syndrome undergoing percutaneous coronary intervention.展开更多
A novel agent (hence termed as P2) was isolated from pig sera in China, which contained covalently bound circular genomic DNAs of 993 nucleotides. Sequence analyses indicated that the agent was closely related to the ...A novel agent (hence termed as P2) was isolated from pig sera in China, which contained covalently bound circular genomic DNAs of 993 nucleotides. Sequence analyses indicated that the agent was closely related to the porcine circovirus (PCV). The molecular clone of P2 was constructed subsequently and used for the following studies. Intracytoplasmic inclusions and intranuclear inclusions were only found in PK-15 cells transfected with the tandem dimer of P2 molecular DNA clone. Intracytoplasmic inclusions were round or irregular in shape and 0.1-0.4 μm in diameter, and intranuclear inclusions were electronically denser than intracytoplasmic inclusions and had two general shapes: round/small (0.1 μm in diameter) and hexagonal/large (0.5―1.4 μm in diameter). The inclusions were not membranously bound. The cells transfected with the tandem dimer of P2 molecular DNA clone were tested positive for P2 DNA at passages 5. The P2 antigen could be detected in both transfected and passaged PK-15 cells. This is the first report regarding the complete nucleotide sequence of a small DNA genome in a circovirus-like infectious agent in vitro.展开更多
A biosorbent prepared from powdered mycelial biomass of Ceriporia lacerata （CLB）, a basidiomycetous fungus, was applied for the uptake of Crystal Violet from aqueous solution. A batch adsorption experiment was used ...A biosorbent prepared from powdered mycelial biomass of Ceriporia lacerata （CLB）, a basidiomycetous fungus, was applied for the uptake of Crystal Violet from aqueous solution. A batch adsorption experiment was used for the biosorption process, involving effect of experimental factors and biosorption kinetics and equilibrium. Biosorption process showed that the removal of Crystal Violet by CLB was effective over wide pH range, and meanwhile was independent on ionic strength. Biosorption capacities of CLB increased with the initial dye concentration increasing, due to an increase in the driving force of the concentration gradient. The adsorbed Crystal Violet amount per unit biomass weight decreased with increasing biosorbent dosage, due to the splitting effect of flux （concentration gradient） between sorbate and biosorbent. A maximum sorption capacity of 239.25 mg/g was observed. Biosorption kinetics was found to be best represented by the pseudo second-order kinetic model. The equilibrium adsorption data was well described by the Koble-Corrigan model. FT-IR （Fourier Transform Infrared Spetroscopy） spectrum showed the presence of O–H, COOH, C=O, C–N, C–H, –NH2 and P–OH in the surface of CLB as functional groups. This study showed CLB can effectively remove CV from dye wastewater.展开更多
文摘采用事件相关电位(Event-Related Potentials,E R P s)方法研究产品设计与性能的冲突对消费者决策过程的影响。在网络购物环境下,消费者对展示了设计与性能的产品进行购买决策,实验结束后根据设计(高审美吸引力/适中审美吸引力)与性能(高/低)是否一致将产品刺激划分为四种组别:组别1(一致:高审美吸引力设计+高性能)、组别2(冲突:高审美吸引力设计+低性能)、组别3(冲突:适中审美吸引力设计+高性能)和组别4(一致:适中审美吸引力设计+低性能)。研究结果显示,组别2和3会在大脑前额皮层诱发波幅较大的负成分N270波,说明消费者根据产品设计形成的性能期望与实际性能不符,形成了刺激与大脑内源信息的冲突,从神经学角度验证了"消费者认为高审美吸引力的设计意味着高性能"。组别1和2会在大脑顶区皮层诱发波幅较大的正成分P2波,这两个组别产品的确定购买率也较高,表明产品设计的美学对消费者的购买决策有正向影响。性能信息也影响着消费者对产品的审美体验,当产品设计的审美吸引力高时,冲突会降低消费者对产品的审美体验,进而降低产品的确定购买率;而当设计的审美吸引力适中时,冲突会提高消费者对产品的审美体验,进而提高产品的确定购买率。对比具有冲突的两个组别,组别2的产品确定购买率高于组别3,说明存在冲突时,审美体验也正向影响消费者的决策。该发现解释了消费者在网络购物时浏览商品照片和规格参数界面时的神经和心理层面的决策过程。
文摘Background The CYP2C19 G681A single polymorphism has been proven to affect clopidogrel responsiveness. However, the effect of coexisting polymorphisms of other genes has not yet been reported in the Chinese population. This study investigated the effect of coexisting polymorphisms of CYP2C19 and P2Y12 on clopidogrel responsiveness and adverse clinical events in Chinese patients. Methods In 577 Han Chinese patients undergoing stent placement because of acute coronary syndrome had platelet reactivity assessed by thromboelastography, and the CYP2C19 G681A and P2Y12 C34T polymorphisms were detected by the ligase detection reaction. Primary clinical endpoints included cardiovascular death, nonfatal myocardial infarction, target vessel revascularization, and stent thrombosis. The secondary clinical endpoints were thrombolysis in myocardial infarction bleeding. The follow-up period was 12 months. Results Genotyping revealed 194 carriers of the wild type GG genotype of CYP2C19 and the wild type CC genotype of P2Y12 （group 1）, 102 carriers of the wild type GG genotype of CYP2C19 and the mutational T allele of P2Y12 （group 2）, 163 carriers of the mutational A allele of CYP2C19 and the wild type CC genotype of P2Y12 （group 3）, and 118 carriers of the mutational A allele of CYP2C19 and the mutational T allele of P2Y12 （group 4）. Group 4 had the lowest ADP-inhibition （49.74＋32.61） and the highest prevalence of clopidogrel low response （29.7%） of the four groups. The rate of the composite of primary clinical endpoints increased more in group 4 （8.5%） than in the other three groups; the rate of composite primary endpoints in group 2 （2.9%） and group 3 （3.7%） were not significantly different than that of group 1 （1.5%）. Conclusion Coexisting polymorphisms of different genes affected clopidogrel responsiveness and clinical outcome more than single polymorphism in Chinese patients with acute coronary syndrome undergoing percutaneous coronary intervention.
基金the State Key Basic Research Project (973 project) of China (Grant No. 2007CB116308)Planned Projects for Postdoctoral Research Funds of Jiangsu Province, China (Grant No. 5910602)Postdoctoral Funds of Jiangsu Academy of Agricultural Sciences, China (Grant No. 6510501)
文摘A novel agent (hence termed as P2) was isolated from pig sera in China, which contained covalently bound circular genomic DNAs of 993 nucleotides. Sequence analyses indicated that the agent was closely related to the porcine circovirus (PCV). The molecular clone of P2 was constructed subsequently and used for the following studies. Intracytoplasmic inclusions and intranuclear inclusions were only found in PK-15 cells transfected with the tandem dimer of P2 molecular DNA clone. Intracytoplasmic inclusions were round or irregular in shape and 0.1-0.4 μm in diameter, and intranuclear inclusions were electronically denser than intracytoplasmic inclusions and had two general shapes: round/small (0.1 μm in diameter) and hexagonal/large (0.5―1.4 μm in diameter). The inclusions were not membranously bound. The cells transfected with the tandem dimer of P2 molecular DNA clone were tested positive for P2 DNA at passages 5. The P2 antigen could be detected in both transfected and passaged PK-15 cells. This is the first report regarding the complete nucleotide sequence of a small DNA genome in a circovirus-like infectious agent in vitro.
基金supported by the Scientific Research Fund of Hunan Provincial Education Department (No.10C1118)the Science and Technology Project of Hunan Province (No. 2009FJ3041)+2 种基金the Project of Natural Science Foundation of Hunan Province (No. 10JJ6053)the Ecological Key Discipline Open Fund of Hunan Province (No.JSS200901)the Project of National Natural Science Foundation of China (No. 31100370)
文摘A biosorbent prepared from powdered mycelial biomass of Ceriporia lacerata （CLB）, a basidiomycetous fungus, was applied for the uptake of Crystal Violet from aqueous solution. A batch adsorption experiment was used for the biosorption process, involving effect of experimental factors and biosorption kinetics and equilibrium. Biosorption process showed that the removal of Crystal Violet by CLB was effective over wide pH range, and meanwhile was independent on ionic strength. Biosorption capacities of CLB increased with the initial dye concentration increasing, due to an increase in the driving force of the concentration gradient. The adsorbed Crystal Violet amount per unit biomass weight decreased with increasing biosorbent dosage, due to the splitting effect of flux （concentration gradient） between sorbate and biosorbent. A maximum sorption capacity of 239.25 mg/g was observed. Biosorption kinetics was found to be best represented by the pseudo second-order kinetic model. The equilibrium adsorption data was well described by the Koble-Corrigan model. FT-IR （Fourier Transform Infrared Spetroscopy） spectrum showed the presence of O–H, COOH, C=O, C–N, C–H, –NH2 and P–OH in the surface of CLB as functional groups. This study showed CLB can effectively remove CV from dye wastewater.
