Several Mn(Ⅲ)10-(hydroxylphenyl)-5,15-bis(pentafluoro-phenyl)corroles with the hydroxyl at ortho-,meta-and para-position of 10-phenyl group were used as the catalysts in the oxidative cleavage of plasmid DNA in the p...Several Mn(Ⅲ)10-(hydroxylphenyl)-5,15-bis(pentafluoro-phenyl)corroles with the hydroxyl at ortho-,meta-and para-position of 10-phenyl group were used as the catalysts in the oxidative cleavage of plasmid DNA in the presence of H2O2.The catalytic system was composed of 10 μL 0.5 mg/mL pC DNA 6 in TE buffer solution(pH=8.0,4.0×10-2 mol/L Tris,1.0×10-3 mol/L EDTA),10 μL 1.0×10-5 mol/L Mn(Ⅲ)corrole in DMSO and 10 μL 0.3% H2O2.After 8 h incubation at room temperature,significant oxidative DNA damage could be observed with the ortho-hydroxyl Mn(Ⅲ)corrole showing a better activity as detected by agarose-gel electrophoresis.Plasmid DNA was completely damaged after 12 h incubation.These observations show Mn(Ⅲ)corrole can be used as an artificial model of nuclease.展开更多
The DNA nuclease-like activity of a water soluble manganese corrole 5, 10, 15-tris（N-methyl-4-pyridyl）- corrolatomanga- nese（III） （MnⅢTMPyC） has been investigated. MnⅢMPyC may bind strongly to DNA via outside ...The DNA nuclease-like activity of a water soluble manganese corrole 5, 10, 15-tris（N-methyl-4-pyridyl）- corrolatomanga- nese（III） （MnⅢTMPyC） has been investigated. MnⅢMPyC may bind strongly to DNA via outside groove binding mode as indicated by absorption spectra, viscosity measurements and CD spectra. MnInTMPyC exhibited excellent catalytic activity in the DNA oxidative cleavage in the presence of hydroperoxide.展开更多
A novel dinuclear copper(Ⅱ) complex, [Cu2(phen)(dipic)2(H2O)2]·2H2O (phen=1,10-phenanthroline, dipicH2=2,6-pyridinedicarboxylic acid), has been prepared and structurally characterized. The complex crystallizes i...A novel dinuclear copper(Ⅱ) complex, [Cu2(phen)(dipic)2(H2O)2]·2H2O (phen=1,10-phenanthroline, dipicH2=2,6-pyridinedicarboxylic acid), has been prepared and structurally characterized. The complex crystallizes in the triclinic system, space group P1 with cell parameters a=0.846 0(17) nm, b=1.289 5(3) nm, c=1.452 7(3) nm, α=77.42(3)°, β=79.11(3)°, γ=87.08(3)°, and V=1.518 8(6) nm3. The dinuclear complex shows potential DNA cleavage activity at micromolar concentration in the presence of H2O2 and exhibits higher nuclease efficiency than mononuclear complex [Cu(dipic)(H2dipic)]·H2O. Without external reductants, the added H2O2 may contribute to the generation of hydroxyl radicals that result in DNA strand scission.CCDC:291786.展开更多
Highly efficient and selective carbon-carbon double bond aerobic cleavage of olefins catalyzed by metallopor- phyrins was investigated, and carbonyl compounds and epoxide were produced as the main products. CoTPP （co...Highly efficient and selective carbon-carbon double bond aerobic cleavage of olefins catalyzed by metallopor- phyrins was investigated, and carbonyl compounds and epoxide were produced as the main products. CoTPP （co- balt meso-tetraphenyl porphyrin） showed excellent activity for the oxidative cleavage of carbon-carbon double bond by using styrene as model compound, in which the TOF （turnover frequency） and selectivity toward benzaldehyde was obtained with 2×10^4h-1 and 86%,respectively.展开更多
文摘Several Mn(Ⅲ)10-(hydroxylphenyl)-5,15-bis(pentafluoro-phenyl)corroles with the hydroxyl at ortho-,meta-and para-position of 10-phenyl group were used as the catalysts in the oxidative cleavage of plasmid DNA in the presence of H2O2.The catalytic system was composed of 10 μL 0.5 mg/mL pC DNA 6 in TE buffer solution(pH=8.0,4.0×10-2 mol/L Tris,1.0×10-3 mol/L EDTA),10 μL 1.0×10-5 mol/L Mn(Ⅲ)corrole in DMSO and 10 μL 0.3% H2O2.After 8 h incubation at room temperature,significant oxidative DNA damage could be observed with the ortho-hydroxyl Mn(Ⅲ)corrole showing a better activity as detected by agarose-gel electrophoresis.Plasmid DNA was completely damaged after 12 h incubation.These observations show Mn(Ⅲ)corrole can be used as an artificial model of nuclease.
基金supported by the National Natural Science Foundation of China(Nos.20771039,20871122, 20971046)National Key Foundation Research Development Project(973)Item of China(No.2007CB815306)
文摘The DNA nuclease-like activity of a water soluble manganese corrole 5, 10, 15-tris（N-methyl-4-pyridyl）- corrolatomanga- nese（III） （MnⅢTMPyC） has been investigated. MnⅢMPyC may bind strongly to DNA via outside groove binding mode as indicated by absorption spectra, viscosity measurements and CD spectra. MnInTMPyC exhibited excellent catalytic activity in the DNA oxidative cleavage in the presence of hydroperoxide.
文摘A novel dinuclear copper(Ⅱ) complex, [Cu2(phen)(dipic)2(H2O)2]·2H2O (phen=1,10-phenanthroline, dipicH2=2,6-pyridinedicarboxylic acid), has been prepared and structurally characterized. The complex crystallizes in the triclinic system, space group P1 with cell parameters a=0.846 0(17) nm, b=1.289 5(3) nm, c=1.452 7(3) nm, α=77.42(3)°, β=79.11(3)°, γ=87.08(3)°, and V=1.518 8(6) nm3. The dinuclear complex shows potential DNA cleavage activity at micromolar concentration in the presence of H2O2 and exhibits higher nuclease efficiency than mononuclear complex [Cu(dipic)(H2dipic)]·H2O. Without external reductants, the added H2O2 may contribute to the generation of hydroxyl radicals that result in DNA strand scission.CCDC:291786.
文摘Highly efficient and selective carbon-carbon double bond aerobic cleavage of olefins catalyzed by metallopor- phyrins was investigated, and carbonyl compounds and epoxide were produced as the main products. CoTPP （co- balt meso-tetraphenyl porphyrin） showed excellent activity for the oxidative cleavage of carbon-carbon double bond by using styrene as model compound, in which the TOF （turnover frequency） and selectivity toward benzaldehyde was obtained with 2×10^4h-1 and 86%,respectively.
