Objective:To analyze effects of high mobility group AT-hook 2(HMGA2)on malignant degree,invasion,metastasis,proliferation and cellular morphology of ovarian cancer cells.Methods:Three methods were applied to observe t...Objective:To analyze effects of high mobility group AT-hook 2(HMGA2)on malignant degree,invasion,metastasis,proliferation and cellular morphology of ovarian cancer cells.Methods:Three methods were applied to observe the effect on HMGA2 expression in ovarian cancer cells and ovarian epithelial cells.Results:After the application of siRNA-HMGA2,number of T29A2-cell clones was decreased,there was significant difference compared with the negative control Block-iT.After application of let-7c,number of T29A2+cell clones was decreased significantly,however,after the application of Anti-let-7,the number of clones restored,and there was no significant difference compared with the negative control group.After interference,the number of T29A2-cells which passed through Matrigel polycarbonate membrane were significantly lower than the negative control group.After the treatment of siRNA-HMGA2,let-7c and sh-HMGA2respectively,growth and proliferation of T29A2-,T29A2+and SKOV3 were slower,and the phenomenon was most obvious in SKOV3.Stable interference of HMGA2 induced mesenchymalepithelial changes in the morphology of SKOV3-sh-HMGA2.Conclusions:HMGA2 can promote malignant transformation of ovarian cancer cells,enhance cell invasion and metastasis,and promote cell growth and proliferation of ovarian cancer cells,which can cause ovarian cancer to progress rapidly and affect the quality of life.展开更多
目的:探讨运动对多囊卵巢综合征(PCOS)大鼠胰岛素、性激素和卵巢形态学的影响。方法:17只21日龄Wistar大鼠随机分为PCOS运动组(n=6)、PCOS非运动组(n=6)和非PCOS对照组(n=5)。注射丙酸睾酮诱导PCOS模型,对照组注射相应容积茶油。运动程...目的:探讨运动对多囊卵巢综合征(PCOS)大鼠胰岛素、性激素和卵巢形态学的影响。方法:17只21日龄Wistar大鼠随机分为PCOS运动组(n=6)、PCOS非运动组(n=6)和非PCOS对照组(n=5)。注射丙酸睾酮诱导PCOS模型,对照组注射相应容积茶油。运动程序为每天游泳2h,连续15d。实验结束后取血测定葡萄糖、胰岛素、雌二醇、黄体酮和睾酮浓度;卵巢病理切片运用图像分析软件Image-Pro Plus 6.0进行形态学分析。结果:PCOS运动组胰岛素、雌二醇和睾酮水平均显著低于非运动组(P<0.05),空腹血糖与空腹胰岛素(FBG/FINS)比值显著高于非运动组(P<0.05),接近于对照组水平。血糖和血清黄体酮水平三组间差异没有显著性。卵巢病理显示,PCOS运动组窦前卵泡数和闭锁卵泡显著减少(P<0.05),而窦状卵泡和黄体数目则显著升高(P<0.05),均接近于对照组水平。结论:短期运动能改善PCOS大鼠胰岛素敏感性,降低血清雄激素和雌激素水平,恢复正常卵巢形态,提示运动是PCOS的基础治疗方法。展开更多
基金supported by National Natural Science Foundation of China(6821183728)
文摘Objective:To analyze effects of high mobility group AT-hook 2(HMGA2)on malignant degree,invasion,metastasis,proliferation and cellular morphology of ovarian cancer cells.Methods:Three methods were applied to observe the effect on HMGA2 expression in ovarian cancer cells and ovarian epithelial cells.Results:After the application of siRNA-HMGA2,number of T29A2-cell clones was decreased,there was significant difference compared with the negative control Block-iT.After application of let-7c,number of T29A2+cell clones was decreased significantly,however,after the application of Anti-let-7,the number of clones restored,and there was no significant difference compared with the negative control group.After interference,the number of T29A2-cells which passed through Matrigel polycarbonate membrane were significantly lower than the negative control group.After the treatment of siRNA-HMGA2,let-7c and sh-HMGA2respectively,growth and proliferation of T29A2-,T29A2+and SKOV3 were slower,and the phenomenon was most obvious in SKOV3.Stable interference of HMGA2 induced mesenchymalepithelial changes in the morphology of SKOV3-sh-HMGA2.Conclusions:HMGA2 can promote malignant transformation of ovarian cancer cells,enhance cell invasion and metastasis,and promote cell growth and proliferation of ovarian cancer cells,which can cause ovarian cancer to progress rapidly and affect the quality of life.
文摘目的:探讨运动对多囊卵巢综合征(PCOS)大鼠胰岛素、性激素和卵巢形态学的影响。方法:17只21日龄Wistar大鼠随机分为PCOS运动组(n=6)、PCOS非运动组(n=6)和非PCOS对照组(n=5)。注射丙酸睾酮诱导PCOS模型,对照组注射相应容积茶油。运动程序为每天游泳2h,连续15d。实验结束后取血测定葡萄糖、胰岛素、雌二醇、黄体酮和睾酮浓度;卵巢病理切片运用图像分析软件Image-Pro Plus 6.0进行形态学分析。结果:PCOS运动组胰岛素、雌二醇和睾酮水平均显著低于非运动组(P<0.05),空腹血糖与空腹胰岛素(FBG/FINS)比值显著高于非运动组(P<0.05),接近于对照组水平。血糖和血清黄体酮水平三组间差异没有显著性。卵巢病理显示,PCOS运动组窦前卵泡数和闭锁卵泡显著减少(P<0.05),而窦状卵泡和黄体数目则显著升高(P<0.05),均接近于对照组水平。结论:短期运动能改善PCOS大鼠胰岛素敏感性,降低血清雄激素和雌激素水平,恢复正常卵巢形态,提示运动是PCOS的基础治疗方法。
