An acute respiratory disease,caused by a novel coronavirus(SARS-CoV-2,previously known as 2019-nCoV),the coronavirus disease 2019(COVID-19)has spread throughout China and received worldwide attention.On 30 January 202...An acute respiratory disease,caused by a novel coronavirus(SARS-CoV-2,previously known as 2019-nCoV),the coronavirus disease 2019(COVID-19)has spread throughout China and received worldwide attention.On 30 January 2020,World Health Organization(WHO)officially declared the COVID-19 epidemic as a public health emergency of international concern.The emergence of SARS-CoV-2,since the severe acute respiratory syndrome coronavirus(SARSCoV)in 2002 and Middle East respiratory syndrome coronavirus(MERS-CoV)in 2012,marked the third introduction of a highly pathogenic and large-scale epidemic coronavirus into the human population in the twenty-first century.As of 1 March 2020,a total of 87,137 confirmed cases globally,79,968 confirmed in China and 7169 outside of China,with 2977 deaths(3.4%)had been reported by WHO.Meanwhile,several independent research groups have identified that SARS-CoV-2 belongs toβ-coronavirus,with highly identical genome to bat coronavirus,pointing to bat as the natural host.The novel coronavirus uses the same receptor,angiotensin-converting enzyme 2(ACE2)as that for SARS-CoV,and mainly spreads through the respiratory tract.Importantly,increasingly evidence showed sustained human-tohuman transmission,along with many exported cases across the globe.The clinical symptoms of COVID-19 patients include fever,cough,fatigue and a small population of patients appeared gastrointestinal infection symptoms.The elderly and people with underlying diseases are susceptible to infection and prone to serious outcomes,which may be associated with acute respiratory distress syndrome(ARDS)and cytokine storm.Currently,there are few specific antiviral strategies,but several potent candidates of antivirals and repurposed drugs are under urgent investigation.In this review,we summarized the latest research progress of the epidemiology,pathogenesis,and clinical characteristics of COVID-19,and discussed the current treatment and scientific advancements to combat the epidemic novel coronavirus.展开更多
Background:Human infections with zoonotic coronaviruses(CoVs),including severe acute respiratory syndrome(SARS)-CoV and Middle East respiratory syndrome(MERS)-CoV,have raised great public health concern globally.Here,...Background:Human infections with zoonotic coronaviruses(CoVs),including severe acute respiratory syndrome(SARS)-CoV and Middle East respiratory syndrome(MERS)-CoV,have raised great public health concern globally.Here,we report a novel batorigin CoV causing severe and fatal pneumonia in humans.Methods:We collected clinical data and bronchoalveolar lavage(BAL)specimens from five patients with severe pneumonia from Wuhan Jinyintan Hospital,Hubei province,China.Nucleic acids of the BAL were extracted and subjected to next-generation sequencing.Virus isolation was carried out,and maximum-likelihood phylogenetic trees were constructed.Results:Five patients hospitalized from December 18 to December 29,2019 presented with fever,cough,and dyspnea accompanied by complications of acute respiratory distress syndrome.Chest radiography revealed diffuse opacities and consolidation.One of these patients died.Sequence results revealed the presence of a previously unknownβ-CoV strain in all five patients,with 99.8%to 99.9%nucleotide identities among the isolates.These isolates showed 79.0%nucleotide identity with the sequence of SARS-CoV(GenBank NC_004718)and 51.8%identity with the sequence of MERS-CoV(GenBank NC_019843).The virus is phylogenetically closest to a bat SARS-like CoV(SL-ZC45,GenBank MG772933)with 87.6%to 87.7%nucleotide identity,but is in a separate clade.Moreover,these viruses have a single intact open reading frame gene 8,as a further indicator of bat-origin CoVs.However,the amino acid sequence of the tentative receptor-binding domain resembles that of SARS-CoV,indicating that these viruses might use the same receptor.Conclusion:A novel bat-borne CoV was identified that is associated with severe and fatal respiratory disease in humans.展开更多
Aiming at the differential distribution of overpressure in vertical and lateral directions in the foreland thrust belt in the southern margin of Junggar Basin,the study on overpressure origin identification and overpr...Aiming at the differential distribution of overpressure in vertical and lateral directions in the foreland thrust belt in the southern margin of Junggar Basin,the study on overpressure origin identification and overpressure evolution simulation is carried out.Based on the measured formation pressure,drilling fluid density and well logging data,overpressure origin identification and overpressure evolution simulation techniques are used to analyze the vertical and lateral distribution patterns of overpressure,genetic mechanisms of overpressure in different structural belts and causes of the differential distribution of overpressure,and the controlling effects of overpressure development and evolution on the formation and distribution of oil and gas reservoirs.The research shows that overpressure occurs in multiple formations vertically in the southern Junggar foreland thrust belt,the deeper the formation,the bigger the scale of the overpressure is.Laterally,overpressure is least developed in the mountain front belt,most developed in the fold anticline belt,and relatively developed in the slope belt.The differential distribution of overpressure is mainly controlled by the differences in disequilibrium compaction and tectonic compression strengths of different belts.The vertical overpressure transmission caused by faults connecting the deep overpressured system has an important contribution to the further increase of the overpressure strength in this area.The controlling effect of overpressure development and evolution on hydrocarbon accumulation and distribution shows in the following aspects:When the strong overpressure was formed before reservoir becoming tight overpressure maintains the physical properties of deep reservoirs to some extent,expanding the exploration depth of deep reservoirs;reservoirs below the overpressured mudstone cap rocks of the Paleogene Anjihaihe Formation and Lower Cretaceous Tugulu Group are main sites for oil and gas accumulation;under the background of overall overpressure,both overpres展开更多
Porcine endogenous retroviruses(PERVs)are proviruses that have medical value in xenotransplantation.However,the evolutionary process leading to the generation of modern PERVs is not well understood.In this study,we in...Porcine endogenous retroviruses(PERVs)are proviruses that have medical value in xenotransplantation.However,the evolutionary process leading to the generation of modern PERVs is not well understood.In this study,we in silico mined 14 pig genomes and other available 304 mammalian genomes,which led to the documentation of 185 full-length PERVs or PERV-like sequences.Notably,we found that lesser Egyptian jerboa(Jaculus jaculus),rock hyrax(Procavia capensis)and eight Muridae species all harbored ancestral PERV-like sequences,indicative of ancient cross-species transmission events from none-porcine species to pigs.We also revealed that 11 genomic rearrangement events were mediated via PERVs,during the process of porcine evolution.In conclusion,these new findings help us to understand the complex evolution of the modern PERVs.展开更多
Based on geographical distribution and literature survey, the present study constructs the historical strata of the appellation words referring to ＂FATHER＂ in Jin Dialects. It holds that the patterns of the appellat...Based on geographical distribution and literature survey, the present study constructs the historical strata of the appellation words referring to ＂FATHER＂ in Jin Dialects. It holds that the patterns of the appellation words are the result of superposition of different historical periods. But their leading words directly inherit the appellation words of standard language of the Song Dynasty and are mainly monosyllabic words. Words used before the Song Dynasty as living fossils of the words only retain in some districts or localities. The different evolution route of＂pa （爸）＂ and ＂tiε （爹）＂ either in Jin dialects or in mandarin is a further proof that Jin dialects and mandarin evolves asynchronously. In addition, it maintains that the formulation of the historical strata is the interaction of the horizontal transmission of languages and systemic changes of local dialects.展开更多
The pandemic of coronavirus disease 2019(COVID-19)has already evoked massive influence.The global pandemic has been ravaging the whole world for a year,with the number of confirmed human infection cases over 150 milli...The pandemic of coronavirus disease 2019(COVID-19)has already evoked massive influence.The global pandemic has been ravaging the whole world for a year,with the number of confirmed human infection cases over 150 million and a death toll exceeding 3 million.Although the genomic sequence of the cognate pathogen SARS-CoV-2(severe acute respiratory syndrome coronavirus 2)has been quickly determined,there are still many unknown aspects,including the virus origin and evolution trend,and the effectiveness of current vaccines and drugs against the mutating virus.This review summarizes current knowledge and advances about COVID-19,including virus origin,transmission and infection,with the aim to improve the understanding of COVID-19 and provide a new perspective for future studies.展开更多
基金by the National Natural Science Foundation of China(81870019)Guangdong Provincial Natural Science Foundation(2018A030313554)+2 种基金National Key R&D Program of China(2018YFC0910601)the National Medical Research Council,Singapore(NMRC/CIRG/1458/2016)a recipient of fellowship support from European Allergy and Clinical Immunology(EAACI)Research Fellowship 2019。
文摘An acute respiratory disease,caused by a novel coronavirus(SARS-CoV-2,previously known as 2019-nCoV),the coronavirus disease 2019(COVID-19)has spread throughout China and received worldwide attention.On 30 January 2020,World Health Organization(WHO)officially declared the COVID-19 epidemic as a public health emergency of international concern.The emergence of SARS-CoV-2,since the severe acute respiratory syndrome coronavirus(SARSCoV)in 2002 and Middle East respiratory syndrome coronavirus(MERS-CoV)in 2012,marked the third introduction of a highly pathogenic and large-scale epidemic coronavirus into the human population in the twenty-first century.As of 1 March 2020,a total of 87,137 confirmed cases globally,79,968 confirmed in China and 7169 outside of China,with 2977 deaths(3.4%)had been reported by WHO.Meanwhile,several independent research groups have identified that SARS-CoV-2 belongs toβ-coronavirus,with highly identical genome to bat coronavirus,pointing to bat as the natural host.The novel coronavirus uses the same receptor,angiotensin-converting enzyme 2(ACE2)as that for SARS-CoV,and mainly spreads through the respiratory tract.Importantly,increasingly evidence showed sustained human-tohuman transmission,along with many exported cases across the globe.The clinical symptoms of COVID-19 patients include fever,cough,fatigue and a small population of patients appeared gastrointestinal infection symptoms.The elderly and people with underlying diseases are susceptible to infection and prone to serious outcomes,which may be associated with acute respiratory distress syndrome(ARDS)and cytokine storm.Currently,there are few specific antiviral strategies,but several potent candidates of antivirals and repurposed drugs are under urgent investigation.In this review,we summarized the latest research progress of the epidemiology,pathogenesis,and clinical characteristics of COVID-19,and discussed the current treatment and scientific advancements to combat the epidemic novel coronavirus.
基金This study was supported by grants from the Chinese Academy of Medical Sciences(CAMS)Innovation Fund for Medical Sciences(No.2016-I2M-1-014)the National Major Science&Technology Project for Control and Prevention of Major Infectious Diseases in China(Nos.2017ZX10103004,2018ZX10305409,2017ZX10204401)the National Natural Science Foundation(No.81930063)
文摘Background:Human infections with zoonotic coronaviruses(CoVs),including severe acute respiratory syndrome(SARS)-CoV and Middle East respiratory syndrome(MERS)-CoV,have raised great public health concern globally.Here,we report a novel batorigin CoV causing severe and fatal pneumonia in humans.Methods:We collected clinical data and bronchoalveolar lavage(BAL)specimens from five patients with severe pneumonia from Wuhan Jinyintan Hospital,Hubei province,China.Nucleic acids of the BAL were extracted and subjected to next-generation sequencing.Virus isolation was carried out,and maximum-likelihood phylogenetic trees were constructed.Results:Five patients hospitalized from December 18 to December 29,2019 presented with fever,cough,and dyspnea accompanied by complications of acute respiratory distress syndrome.Chest radiography revealed diffuse opacities and consolidation.One of these patients died.Sequence results revealed the presence of a previously unknownβ-CoV strain in all five patients,with 99.8%to 99.9%nucleotide identities among the isolates.These isolates showed 79.0%nucleotide identity with the sequence of SARS-CoV(GenBank NC_004718)and 51.8%identity with the sequence of MERS-CoV(GenBank NC_019843).The virus is phylogenetically closest to a bat SARS-like CoV(SL-ZC45,GenBank MG772933)with 87.6%to 87.7%nucleotide identity,but is in a separate clade.Moreover,these viruses have a single intact open reading frame gene 8,as a further indicator of bat-origin CoVs.However,the amino acid sequence of the tentative receptor-binding domain resembles that of SARS-CoV,indicating that these viruses might use the same receptor.Conclusion:A novel bat-borne CoV was identified that is associated with severe and fatal respiratory disease in humans.
基金PetroChina Science and Technology Development Project(2021DJ0105,2021DJ0203,2021DJ0303)National Natural Science Foundation of China(42172164,42002177)。
文摘Aiming at the differential distribution of overpressure in vertical and lateral directions in the foreland thrust belt in the southern margin of Junggar Basin,the study on overpressure origin identification and overpressure evolution simulation is carried out.Based on the measured formation pressure,drilling fluid density and well logging data,overpressure origin identification and overpressure evolution simulation techniques are used to analyze the vertical and lateral distribution patterns of overpressure,genetic mechanisms of overpressure in different structural belts and causes of the differential distribution of overpressure,and the controlling effects of overpressure development and evolution on the formation and distribution of oil and gas reservoirs.The research shows that overpressure occurs in multiple formations vertically in the southern Junggar foreland thrust belt,the deeper the formation,the bigger the scale of the overpressure is.Laterally,overpressure is least developed in the mountain front belt,most developed in the fold anticline belt,and relatively developed in the slope belt.The differential distribution of overpressure is mainly controlled by the differences in disequilibrium compaction and tectonic compression strengths of different belts.The vertical overpressure transmission caused by faults connecting the deep overpressured system has an important contribution to the further increase of the overpressure strength in this area.The controlling effect of overpressure development and evolution on hydrocarbon accumulation and distribution shows in the following aspects:When the strong overpressure was formed before reservoir becoming tight overpressure maintains the physical properties of deep reservoirs to some extent,expanding the exploration depth of deep reservoirs;reservoirs below the overpressured mudstone cap rocks of the Paleogene Anjihaihe Formation and Lower Cretaceous Tugulu Group are main sites for oil and gas accumulation;under the background of overall overpressure,both overpres
基金supported by the Special Key Project of Biosafety Technologies(2017YFC1200800)for the National Major Research&Development Program of ChinaJ.C.is supported by National Natural Science Foundation of China under grant no.31671324 and CAS Pioneer Hundred Talents Program.
文摘Porcine endogenous retroviruses(PERVs)are proviruses that have medical value in xenotransplantation.However,the evolutionary process leading to the generation of modern PERVs is not well understood.In this study,we in silico mined 14 pig genomes and other available 304 mammalian genomes,which led to the documentation of 185 full-length PERVs or PERV-like sequences.Notably,we found that lesser Egyptian jerboa(Jaculus jaculus),rock hyrax(Procavia capensis)and eight Muridae species all harbored ancestral PERV-like sequences,indicative of ancient cross-species transmission events from none-porcine species to pigs.We also revealed that 11 genomic rearrangement events were mediated via PERVs,during the process of porcine evolution.In conclusion,these new findings help us to understand the complex evolution of the modern PERVs.
文摘Based on geographical distribution and literature survey, the present study constructs the historical strata of the appellation words referring to ＂FATHER＂ in Jin Dialects. It holds that the patterns of the appellation words are the result of superposition of different historical periods. But their leading words directly inherit the appellation words of standard language of the Song Dynasty and are mainly monosyllabic words. Words used before the Song Dynasty as living fossils of the words only retain in some districts or localities. The different evolution route of＂pa （爸）＂ and ＂tiε （爹）＂ either in Jin dialects or in mandarin is a further proof that Jin dialects and mandarin evolves asynchronously. In addition, it maintains that the formulation of the historical strata is the interaction of the horizontal transmission of languages and systemic changes of local dialects.
基金supported by the Strategic Priority Research Program of CAS(XDB29010000)the National Science and Technology Major Project(2018ZX10101004)+5 种基金National Key Research and Development Program of China(2020YFC0845900)the National Natural Science Foundation of China(NSFC)(82041016,81871658 and 81802010)a grant from the Bill&Melinda Gates Foundationis partially supported by the Yanqi Lake Meeting organized by the Academic Divisions of CASR.P.is supported by the Young Elite Scientist Sponsorship Program(YESS)by China Association for Science and Technology(CAST)(2018QNRC001)Y.S.is also supported by the Youth Innovation Promotion Association of CAS.
文摘The pandemic of coronavirus disease 2019(COVID-19)has already evoked massive influence.The global pandemic has been ravaging the whole world for a year,with the number of confirmed human infection cases over 150 million and a death toll exceeding 3 million.Although the genomic sequence of the cognate pathogen SARS-CoV-2(severe acute respiratory syndrome coronavirus 2)has been quickly determined,there are still many unknown aspects,including the virus origin and evolution trend,and the effectiveness of current vaccines and drugs against the mutating virus.This review summarizes current knowledge and advances about COVID-19,including virus origin,transmission and infection,with the aim to improve the understanding of COVID-19 and provide a new perspective for future studies.
