[Objective] The experiment aimed to inquire into the regular pattern of the flavonoids content in erect type Ophiopogon jaonicus preliminarily.[Methods]In the main Ophiopogon jaonicus-producing area,we use the method ...[Objective] The experiment aimed to inquire into the regular pattern of the flavonoids content in erect type Ophiopogon jaonicus preliminarily.[Methods]In the main Ophiopogon jaonicus-producing area,we use the method of home position dynamic method to gather sample for experiments,use UV to determine the flavonoids content in Ophiopogon jaonicus and use correlation and regression method to analyze the relation between flavonoids content and the growth period of Ophiopogon jaonicus.[Results]The relation between flavonoids content(y1)and the growth period(x∥d)of erect type Ophiopogon jaonicus is:y=1.235 6lnx-4.748 9(n=7,50≤x≤730,0≤y≤3.128,r0.001=0.950 7,r=0.954 2).[Conclusion]During a determinate time(50≤x≤730),positive correlation exists between flavonoids content and the growth period of Ophiopogon jaonicus.Our research has a great importance in directing the scientific harvest and GAP production of erect type Ophiopogon jaonicus.展开更多
A new C27-steroidal glycoside with three known compounds were isolated from the tuber of Ophiopogonjaponicus （Thunb.） Ker-Gawl. Based on the spectral analysis, including HRMS, 1H NMR, 13C NMR, DEPT, 1H-1H COSY, HMQC...A new C27-steroidal glycoside with three known compounds were isolated from the tuber of Ophiopogonjaponicus （Thunb.） Ker-Gawl. Based on the spectral analysis, including HRMS, 1H NMR, 13C NMR, DEPT, 1H-1H COSY, HMQC and HMBC, their chemical structures were determined as pennogenin-3-O-α-L-rhamnopyranosyl-（1→ 2）-β-D-xylopyranosyl-（1→ 4）-13-D-gluco- pyranoside （1）, pennogenin-3-O-α-L-rhamnopyranosyl-（1→ 2）-β-D-glucopyranoside （2）, ophiopojaponin C （3） and ophiopogonin D （4）. 2007 Jian Zhong Wang. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All rights reserved.展开更多
Two new C-27 steroidal: glycosides, named ophiopojaponin A(1)and B(2), were isolated from the tubers of famous traditional Chinese herb-Ophiopogon japonicus. The spectroscopic and chemical evidences revealed their str...Two new C-27 steroidal: glycosides, named ophiopojaponin A(1)and B(2), were isolated from the tubers of famous traditional Chinese herb-Ophiopogon japonicus. The spectroscopic and chemical evidences revealed their structures to be Pennogenin 3-O-[2'-O-acetyl-alpha-L-rhamnopyranosyl (1-->2)]-beta-D-xylopyranosyl (1-->3)-beta-D-glucopyranoside (1) and 26-O-beta-D-glucopyranosyl-(22 xi, 25R)-3 beta, 14 alpha, 22 xi, 26-tetrahydroxyfurost-5-ene 3-O-alpha-L-rhamnopyranosyl (1-->2)-beta-D-glucopyranoside (2), respectively.展开更多
Ultrafiltration is one of the most fascinating technologies, which makes it possible to improve the quality of traditional medicines for application in the pharmaceutical industry. However, researchers have paid littl...Ultrafiltration is one of the most fascinating technologies, which makes it possible to improve the quality of traditional medicines for application in the pharmaceutical industry. However, researchers have paid little attention to the effect of ultrafiltration membrane on traditional medicines chemical constituents. In this work, Ophiopogon japonicus(L.f) Ker-Gawl. was used as an example to illuminate the influence of ultrafiltration with different material and molecular weight cut-off(MWCO) membrane on natural chemical constituents as measured by ultra-fast liquid chromatography coupled with ion trap time-of-flight mass spectrometry(UFLC-IT-TOF/MS). Our results indicated that ultrafiltration membrane significantly impacted homoisoflavonoids, especially homoisoflavonoids that were almost completely retained on the polyethersulfone(PES) membrane. We also found that the larger number of aglycone hydroxy and sugar moiety in steroid saponins, the higher the transmittance. Furthermore, the passage rate(%) of ophiogenin type saponins was higher than that of others. The possible adsorptive mechanisms were hydrogen bonding, hydrophobic interactions, and benzene ring interaction by π-π stacking. In conclusion, it is crucial to choose appropriate ultrafiltration membrane based on the characteristics of produce products for application of ultrafiltration technique.展开更多
基金Supported by Crop Breeding Key Project of Eleventh Five Year Plan in Sichuan Province(2006yzgg12-9)~~
文摘[Objective] The experiment aimed to inquire into the regular pattern of the flavonoids content in erect type Ophiopogon jaonicus preliminarily.[Methods]In the main Ophiopogon jaonicus-producing area,we use the method of home position dynamic method to gather sample for experiments,use UV to determine the flavonoids content in Ophiopogon jaonicus and use correlation and regression method to analyze the relation between flavonoids content and the growth period of Ophiopogon jaonicus.[Results]The relation between flavonoids content(y1)and the growth period(x∥d)of erect type Ophiopogon jaonicus is:y=1.235 6lnx-4.748 9(n=7,50≤x≤730,0≤y≤3.128,r0.001=0.950 7,r=0.954 2).[Conclusion]During a determinate time(50≤x≤730),positive correlation exists between flavonoids content and the growth period of Ophiopogon jaonicus.Our research has a great importance in directing the scientific harvest and GAP production of erect type Ophiopogon jaonicus.
文摘A new C27-steroidal glycoside with three known compounds were isolated from the tuber of Ophiopogonjaponicus （Thunb.） Ker-Gawl. Based on the spectral analysis, including HRMS, 1H NMR, 13C NMR, DEPT, 1H-1H COSY, HMQC and HMBC, their chemical structures were determined as pennogenin-3-O-α-L-rhamnopyranosyl-（1→ 2）-β-D-xylopyranosyl-（1→ 4）-13-D-gluco- pyranoside （1）, pennogenin-3-O-α-L-rhamnopyranosyl-（1→ 2）-β-D-glucopyranoside （2）, ophiopojaponin C （3） and ophiopogonin D （4）. 2007 Jian Zhong Wang. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All rights reserved.
基金a key project of Chinese Academy of sciences. We are grateful to the members of Instrument Group of Phytochemistry Laboratory, K
文摘Two new C-27 steroidal: glycosides, named ophiopojaponin A(1)and B(2), were isolated from the tubers of famous traditional Chinese herb-Ophiopogon japonicus. The spectroscopic and chemical evidences revealed their structures to be Pennogenin 3-O-[2'-O-acetyl-alpha-L-rhamnopyranosyl (1-->2)]-beta-D-xylopyranosyl (1-->3)-beta-D-glucopyranoside (1) and 26-O-beta-D-glucopyranosyl-(22 xi, 25R)-3 beta, 14 alpha, 22 xi, 26-tetrahydroxyfurost-5-ene 3-O-alpha-L-rhamnopyranosyl (1-->2)-beta-D-glucopyranoside (2), respectively.
基金supported by the National Natural Science Foundation of China(Nos.81473317,81274004)2011 Program for Excellent Scientific and Technological Innovation Team of Jiangsu Higher Educationa Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions
文摘Ultrafiltration is one of the most fascinating technologies, which makes it possible to improve the quality of traditional medicines for application in the pharmaceutical industry. However, researchers have paid little attention to the effect of ultrafiltration membrane on traditional medicines chemical constituents. In this work, Ophiopogon japonicus(L.f) Ker-Gawl. was used as an example to illuminate the influence of ultrafiltration with different material and molecular weight cut-off(MWCO) membrane on natural chemical constituents as measured by ultra-fast liquid chromatography coupled with ion trap time-of-flight mass spectrometry(UFLC-IT-TOF/MS). Our results indicated that ultrafiltration membrane significantly impacted homoisoflavonoids, especially homoisoflavonoids that were almost completely retained on the polyethersulfone(PES) membrane. We also found that the larger number of aglycone hydroxy and sugar moiety in steroid saponins, the higher the transmittance. Furthermore, the passage rate(%) of ophiogenin type saponins was higher than that of others. The possible adsorptive mechanisms were hydrogen bonding, hydrophobic interactions, and benzene ring interaction by π-π stacking. In conclusion, it is crucial to choose appropriate ultrafiltration membrane based on the characteristics of produce products for application of ultrafiltration technique.
