[Objective] This study aimed to predict the structure of protein OmpH from Pasteurella multocida C47-8 （PmC47-8） strain of yak. [Method] Online BLAST, signal peptide prediction, secondary structure prediction and pr...[Objective] This study aimed to predict the structure of protein OmpH from Pasteurella multocida C47-8 （PmC47-8） strain of yak. [Method] Online BLAST, signal peptide prediction, secondary structure prediction and protein characteristics of sequencing result of gene OmpH from PmC47-8 strain were analyzed. [Result] The similarities of gene OmpH from PmC47-8 with the published 81 OmpH genes were between 84% and 99%; a signal peptide was found with the cleavage sites between 20 and 21 in the polypeptide; secondary structure prediction showed that folding structure accounted for 49.8% and loop structure for 50.2%; it predicted that there were 7 O-glycosylation sites in OmpH protein with the amino acid residual sites of 2, 45, 48, 330, 716, 721, 723, respectively, and 2 N-glycosylation sites with the amino acid residual sites of 15 and 35. [Conclusion] This study lays the foundation for the study on the immunity of OmpH gene from yak.展开更多
本试验旨在构建贝氏柯克斯体外膜蛋白H(outer membrance protein H,OmpH)的重组表达载体并分析该蛋白的免疫原性,以贝氏柯克斯体九里株为模板,通过PCR方法扩增出含798bp的贝氏柯克斯体的OmpH基因片段,并将其克隆至原核表达载体pQE-30,...本试验旨在构建贝氏柯克斯体外膜蛋白H(outer membrance protein H,OmpH)的重组表达载体并分析该蛋白的免疫原性,以贝氏柯克斯体九里株为模板,通过PCR方法扩增出含798bp的贝氏柯克斯体的OmpH基因片段,并将其克隆至原核表达载体pQE-30,得到重组表达质粒pQE-30/OmpH。经IPTG诱导后,SDS-PAGE结果发现,该重组蛋白大小约为25ku。Western blotting试验结果显示,该方法所诱导产生的重组蛋白具有良好反应原性。展开更多
基金Supported by the Project for High-level Talents of Qinghai University (2008-QGC-7)~~
文摘[Objective] This study aimed to predict the structure of protein OmpH from Pasteurella multocida C47-8 （PmC47-8） strain of yak. [Method] Online BLAST, signal peptide prediction, secondary structure prediction and protein characteristics of sequencing result of gene OmpH from PmC47-8 strain were analyzed. [Result] The similarities of gene OmpH from PmC47-8 with the published 81 OmpH genes were between 84% and 99%; a signal peptide was found with the cleavage sites between 20 and 21 in the polypeptide; secondary structure prediction showed that folding structure accounted for 49.8% and loop structure for 50.2%; it predicted that there were 7 O-glycosylation sites in OmpH protein with the amino acid residual sites of 2, 45, 48, 330, 716, 721, 723, respectively, and 2 N-glycosylation sites with the amino acid residual sites of 15 and 35. [Conclusion] This study lays the foundation for the study on the immunity of OmpH gene from yak.
文摘本试验旨在构建贝氏柯克斯体外膜蛋白H(outer membrance protein H,OmpH)的重组表达载体并分析该蛋白的免疫原性,以贝氏柯克斯体九里株为模板,通过PCR方法扩增出含798bp的贝氏柯克斯体的OmpH基因片段,并将其克隆至原核表达载体pQE-30,得到重组表达质粒pQE-30/OmpH。经IPTG诱导后,SDS-PAGE结果发现,该重组蛋白大小约为25ku。Western blotting试验结果显示,该方法所诱导产生的重组蛋白具有良好反应原性。
