Off-season reproduction is a favorable economic trait for sheep industry. Hu sheep, an indigenous Chinese sheep breed, demonstrates a higher productivity of lambs and displays year-around oestrous behavior under prope...Off-season reproduction is a favorable economic trait for sheep industry. Hu sheep, an indigenous Chinese sheep breed, demonstrates a higher productivity of lambs and displays year-around oestrous behavior under proper nutrition and environment. The genetic basis behind these traits, however, is not well understood. In order to identify genes associated with the off-season reproduction, we constructed a suppression subtractive hybridization （SSH） cDNA library using pooled ovary mRNAs of 6 oestrous Hu females as a tester and the pooled ovary mRNAs of 6 non-oestrous Chinese Merino females as a driver. A total of 382 resulting positive clones were obtained after the SSH. We identified 114 differentially up-regulated genes in oestrous Hu sheep by using subsequent screening and DNA sequencing, of which 8 were previously known, 93 were reported for the first time in sheep, and 13 were novel with no significant homology to any sequence in the DNA databases. Functions of the genes identified are related to cell division, signal transduction, structure, metabolism, or cell defense. To validate the results of SSH, 6 genes （Ntrk2, Ppap2b, Htral, Nidl, Serpine2 and Foxola） were selected for conformational analysis using quantitative real-time PCR （qRT-PCR）, and two of them （Htral and Foxola） were verified by Northern blot. All of the 6 genes were differentially up-regulated in the ovary of oestrous Hu. It is obvious that off-season reproduction is a complex trait involving multiple genes in multiple organs. This study helps to provide a foundation for the final identification of functional genes involved in the sheep ovary.展开更多
Objective:To determine the effects of a glyphosate-based herbicide on the oestrous cycle of rats.Methods:Fifteen adult Wistar rats were randomly divided into three groups.Group A served as the control group,while grou...Objective:To determine the effects of a glyphosate-based herbicide on the oestrous cycle of rats.Methods:Fifteen adult Wistar rats were randomly divided into three groups.Group A served as the control group,while groups B and C received 250 and 500 mg/kg of glyphosate-based herbicide orally for five oestrous cycles,respectively.Stages of oestrous cycle,oestrous cycle index,length of cycle,oestrous cycle ratio,serum estradiol and progesterone levels were determined.Results:The proestrus and oestrus stages of the glyphosate-based herbicide groups increased significantly(P<0.05),although proestrus in group C was not significantly different from the control group.There was a significant decrease in the metestrus and diestrus of the glyphosate-based herbicide groups(P<0.05).The oestrous cycle index of the glyphosate-based herbicide treated groups was altered;this was characterized by an increase in the oestrous index and a decrease in the metestrus and diestrus indexes.The proestrus index of group B increased,while that of group C decreased.The length of the cycle of the glyphosate-based herbicide groups significantly decreased from the 1st and 3rd week till the end of the study in groups B and C,respectively(P<0.05).There was a significant increase in the oestrous cycle ratio of the glyphosate-based herbicide groups compared to the control group(P<0.05).Though the estradiol and progesterone levels of the glyphosate-based herbicide groups increased and decreased,respectively,they were not significantly different from the control group.Conclusions:Glyphosate-based herbicide at the doses of 250 and 500 mg/kg can alter the pattern of the oestrous cycle in rats.展开更多
Background: DNA methylation plays a vital role in reproduction.Entire genome DNA methylation changes during the oestrous phase(ES) and dioestrous phase(DS) in the ovaries of Guanzhong dairy goats were investigated usi...Background: DNA methylation plays a vital role in reproduction.Entire genome DNA methylation changes during the oestrous phase(ES) and dioestrous phase(DS) in the ovaries of Guanzhong dairy goats were investigated using bisulphite sequencing to understand the molecular biological mechanisms of these goats' oestrous cycle.Results: We discovered distinct genome-wide DNA methylation patterns in ES and DS ovaries.A total of 26,910 differentially methylated regions were upregulated and 21,453 differentially methylated regions were downregulated in the ES samples compared with the DS samples(P-values ≤0.05 and fold change of methylation ratios ≥2).Differentially methylated region analysis showed hypomethylation in the gene body regions and hypermethylation in the joining region between upstream regions and gene bodies.The methylation ratios of the STAR,FGF2,FGF12,BMP5 and SMAD6 genes in the ES samples were lower than those of the DS samples(P-values≤0.05 and fold change of methylation ratios ≥2).Conversely,the methylation ratios of the EGFR,TGFBR2,IGF2 BP1 and MMD2 genes increased in the ES samples compared with the DS samples.In addition,223 differentially methylated genes were found in the GnRH signalling pathway(KO04912),ovarian steroidogenesis pathway(KO04913),oestrogen signalling pathway(KO04915),oxytocin signalling pathway(KO04921),insulin secretion pathway(KO04911) and MAPK signalling pathway(KO04010).Conclusions: This study is the first large-scale comparison of the high-resolution DNA methylation landscapes of oestrous and dioestrous ovaries from dairy goats.Previous studies and our investigations have shown that the NR5 A2,STAR,FGF2 and BMP5 genes might have potential application value in regulating caprine oestrus.展开更多
Objective: To evaluate the effects of Eurycoma longifolia(E. longifolia) standardized extract on the oestrous cycle, levels of reproductive hormones and histology of the ovaries of Sprague-Dawley rats.Methods: Female ...Objective: To evaluate the effects of Eurycoma longifolia(E. longifolia) standardized extract on the oestrous cycle, levels of reproductive hormones and histology of the ovaries of Sprague-Dawley rats.Methods: Female rats were orally treated with E. longifolia standardized extract at the dose levels of 2.5, 5.0, 10.0, 25.0, 50.0 and 100.0 mg/kg of body weight over 5 days.Vaginal smears were monitored daily within the duration and after withdrawal of the treatment before being sacrificed. The body weights of the females were recorded before and after the 5 days treatment. At the end of the experiments, blood samples were collected for determination of testosterone, oestradiol and progesterone levels. Ovaries were removed, weighed and examined for histomorphological changes.Results: The administration of E. longifolia standardized extract did not significantly alter the oestrous cycle of the rats during the 5 days treatment and after withdrawal of the treatments. This was supported by normal testosterone, oestradiol and progesterone levels as well as normal morphology of the ovaries.Conclusions: The data obtained showed that E. longifolia standardized extract did not exhibit any toxic effect on reproductive activities of female rats suggesting potential use in the management of infertility.展开更多
选取苏钟种母猪16头,在第2个发情期后,按发情前期、发情期、发情后期和间情期随机分成4组。用RT-PCR检测苏钟猪发情周期不同时期preproorexin和orexin 1受体(OX1R) m RNA在下丘脑-垂体-卵巢轴中的表达。结果显示:发情周期不同时期prepro...选取苏钟种母猪16头,在第2个发情期后,按发情前期、发情期、发情后期和间情期随机分成4组。用RT-PCR检测苏钟猪发情周期不同时期preproorexin和orexin 1受体(OX1R) m RNA在下丘脑-垂体-卵巢轴中的表达。结果显示:发情周期不同时期preproorexin m RNA在下丘脑、垂体和卵巢中变化趋势一致,preproorexin m RNA在猪的发情前期表达最高,随后其表达量开始下降,在发情后期其表达最少,在间情期时又开始上升。下丘脑中OX1R m RNA在发情前期开始上升,在发情后期达到最高,随后在间情期又开始下降。垂体和卵巢中OX1 R m RNA的变化与下丘脑中OX1R m RNA的变化趋势一致。上述结果表明:orexin可能参与调控动物生殖过程。展开更多
基金supported by the grants from the Ministry of Science and Technology of China(No. 2007DFB30420)the China Ministry of Agriculture (No.2009ZX08008-005B)
文摘Off-season reproduction is a favorable economic trait for sheep industry. Hu sheep, an indigenous Chinese sheep breed, demonstrates a higher productivity of lambs and displays year-around oestrous behavior under proper nutrition and environment. The genetic basis behind these traits, however, is not well understood. In order to identify genes associated with the off-season reproduction, we constructed a suppression subtractive hybridization （SSH） cDNA library using pooled ovary mRNAs of 6 oestrous Hu females as a tester and the pooled ovary mRNAs of 6 non-oestrous Chinese Merino females as a driver. A total of 382 resulting positive clones were obtained after the SSH. We identified 114 differentially up-regulated genes in oestrous Hu sheep by using subsequent screening and DNA sequencing, of which 8 were previously known, 93 were reported for the first time in sheep, and 13 were novel with no significant homology to any sequence in the DNA databases. Functions of the genes identified are related to cell division, signal transduction, structure, metabolism, or cell defense. To validate the results of SSH, 6 genes （Ntrk2, Ppap2b, Htral, Nidl, Serpine2 and Foxola） were selected for conformational analysis using quantitative real-time PCR （qRT-PCR）, and two of them （Htral and Foxola） were verified by Northern blot. All of the 6 genes were differentially up-regulated in the ovary of oestrous Hu. It is obvious that off-season reproduction is a complex trait involving multiple genes in multiple organs. This study helps to provide a foundation for the final identification of functional genes involved in the sheep ovary.
文摘Objective:To determine the effects of a glyphosate-based herbicide on the oestrous cycle of rats.Methods:Fifteen adult Wistar rats were randomly divided into three groups.Group A served as the control group,while groups B and C received 250 and 500 mg/kg of glyphosate-based herbicide orally for five oestrous cycles,respectively.Stages of oestrous cycle,oestrous cycle index,length of cycle,oestrous cycle ratio,serum estradiol and progesterone levels were determined.Results:The proestrus and oestrus stages of the glyphosate-based herbicide groups increased significantly(P<0.05),although proestrus in group C was not significantly different from the control group.There was a significant decrease in the metestrus and diestrus of the glyphosate-based herbicide groups(P<0.05).The oestrous cycle index of the glyphosate-based herbicide treated groups was altered;this was characterized by an increase in the oestrous index and a decrease in the metestrus and diestrus indexes.The proestrus index of group B increased,while that of group C decreased.The length of the cycle of the glyphosate-based herbicide groups significantly decreased from the 1st and 3rd week till the end of the study in groups B and C,respectively(P<0.05).There was a significant increase in the oestrous cycle ratio of the glyphosate-based herbicide groups compared to the control group(P<0.05).Though the estradiol and progesterone levels of the glyphosate-based herbicide groups increased and decreased,respectively,they were not significantly different from the control group.Conclusions:Glyphosate-based herbicide at the doses of 250 and 500 mg/kg can alter the pattern of the oestrous cycle in rats.
基金supported by the National Natural Science Foundation of China(31601925)China postdoctoral science foundation(2016 T90954)
文摘Background: DNA methylation plays a vital role in reproduction.Entire genome DNA methylation changes during the oestrous phase(ES) and dioestrous phase(DS) in the ovaries of Guanzhong dairy goats were investigated using bisulphite sequencing to understand the molecular biological mechanisms of these goats' oestrous cycle.Results: We discovered distinct genome-wide DNA methylation patterns in ES and DS ovaries.A total of 26,910 differentially methylated regions were upregulated and 21,453 differentially methylated regions were downregulated in the ES samples compared with the DS samples(P-values ≤0.05 and fold change of methylation ratios ≥2).Differentially methylated region analysis showed hypomethylation in the gene body regions and hypermethylation in the joining region between upstream regions and gene bodies.The methylation ratios of the STAR,FGF2,FGF12,BMP5 and SMAD6 genes in the ES samples were lower than those of the DS samples(P-values≤0.05 and fold change of methylation ratios ≥2).Conversely,the methylation ratios of the EGFR,TGFBR2,IGF2 BP1 and MMD2 genes increased in the ES samples compared with the DS samples.In addition,223 differentially methylated genes were found in the GnRH signalling pathway(KO04912),ovarian steroidogenesis pathway(KO04913),oestrogen signalling pathway(KO04915),oxytocin signalling pathway(KO04921),insulin secretion pathway(KO04911) and MAPK signalling pathway(KO04010).Conclusions: This study is the first large-scale comparison of the high-resolution DNA methylation landscapes of oestrous and dioestrous ovaries from dairy goats.Previous studies and our investigations have shown that the NR5 A2,STAR,FGF2 and BMP5 genes might have potential application value in regulating caprine oestrus.
基金Supported by National Key Economic Area Research Grant Scheme,NRGS(Grant No.SP15-061-0183)provided by Ministry of Agriculture and Agro-based Industry,Malaysia
文摘Objective: To evaluate the effects of Eurycoma longifolia(E. longifolia) standardized extract on the oestrous cycle, levels of reproductive hormones and histology of the ovaries of Sprague-Dawley rats.Methods: Female rats were orally treated with E. longifolia standardized extract at the dose levels of 2.5, 5.0, 10.0, 25.0, 50.0 and 100.0 mg/kg of body weight over 5 days.Vaginal smears were monitored daily within the duration and after withdrawal of the treatment before being sacrificed. The body weights of the females were recorded before and after the 5 days treatment. At the end of the experiments, blood samples were collected for determination of testosterone, oestradiol and progesterone levels. Ovaries were removed, weighed and examined for histomorphological changes.Results: The administration of E. longifolia standardized extract did not significantly alter the oestrous cycle of the rats during the 5 days treatment and after withdrawal of the treatments. This was supported by normal testosterone, oestradiol and progesterone levels as well as normal morphology of the ovaries.Conclusions: The data obtained showed that E. longifolia standardized extract did not exhibit any toxic effect on reproductive activities of female rats suggesting potential use in the management of infertility.
文摘选取苏钟种母猪16头,在第2个发情期后,按发情前期、发情期、发情后期和间情期随机分成4组。用RT-PCR检测苏钟猪发情周期不同时期preproorexin和orexin 1受体(OX1R) m RNA在下丘脑-垂体-卵巢轴中的表达。结果显示:发情周期不同时期preproorexin m RNA在下丘脑、垂体和卵巢中变化趋势一致,preproorexin m RNA在猪的发情前期表达最高,随后其表达量开始下降,在发情后期其表达最少,在间情期时又开始上升。下丘脑中OX1R m RNA在发情前期开始上升,在发情后期达到最高,随后在间情期又开始下降。垂体和卵巢中OX1 R m RNA的变化与下丘脑中OX1R m RNA的变化趋势一致。上述结果表明:orexin可能参与调控动物生殖过程。
