Liver transplantation (LT) is the best treatment for end-stage hepatic failure, with an excellent survival rates over the last decade. Biliary complications after LT pose a major challenge especially with the increasi...Liver transplantation (LT) is the best treatment for end-stage hepatic failure, with an excellent survival rates over the last decade. Biliary complications after LT pose a major challenge especially with the increasing number of procured organs after circulatory death. Ischaemic cholangiopathy (IC) is a set of disorders characterized by multiple diffuse strictures affecting the graft biliary system in the absence of hepatic artery thrombosis or stenosis. It commonly presents with cholestasis and cholangitis resulting in higher readmission rates, longer length of stay, repeated therapeutic interventions, and eventually re-transplantation with consequent effects on the patient’s quality of life and increased health care costs. The pathogenesis of IC is unclear and exhibits a higher prevalence with prolonged ischaemia time, donation after circulatory death (DCD), rejection, and cytomegalovirus infection. The majority of IC occurs within 12 mo after LT. Prolonged warm ischaemic times predispose to a profound injury with a subsequently higher prevalence of IC. Biliary complications and IC rates are between 16% and 29% in DCD grafts compared to between 3% and 17% in donation after brain death (DBD) grafts. The majority of ischaemic biliary lesions occur within 30 d in DCD compared to 90 d in DBD grafts following transplantation. However, there are many other risk factors for IC that should be considered. The benefits of DCD in expanding the donor pool are hindered by the higher incidence of IC with increased rates of re-transplantation. Careful donor selection and procurement might help to optimize the utilization of DCD grafts.展开更多
Seasonal outbreaks of airsacculitis in China＇s poultry cause great economic losses annually. This study tried to unveil the potential role of Avian metopneumovirus （AM PV）, Ornithobacterium rhinotrocheale （aRT） a...Seasonal outbreaks of airsacculitis in China＇s poultry cause great economic losses annually. This study tried to unveil the potential role of Avian metopneumovirus （AM PV）, Ornithobacterium rhinotrocheale （aRT） and Chlarnydia psittaci （CPS） in avian airsacculitis. A serological investigation of 673 breeder chickens and a case-controlled study of 430 birds were undertaken. Results showed that infection with AMPV, aRT, and CPS was highly associated with the disease. The correlation between AMPV and CPS were positively robust in both layers and broilers. Finally, we determined the co-infection with AMPV, aRT, and CPS was prevalent in the sampled poultry farms suffering from respiratory diseases and the outbreak of airsacculitis was closely related to simultaneous exposure to all three agents.展开更多
The study was to determine the appropriate dose and administration route of Ornitin Triple vaccine in cross-breed coloured broilers in Vietnam by evaluating the antibody titer against Ornithobacterium rhinotracheale(O...The study was to determine the appropriate dose and administration route of Ornitin Triple vaccine in cross-breed coloured broilers in Vietnam by evaluating the antibody titer against Ornithobacterium rhinotracheale(ORT)and local reactions at injection sites on chickens after vaccination.The study was divided into 2 trials.Both trials were designed with 3 vaccine dose groups:0.0ml(control group),0.25ml and 0.5ml and 2 different administration routes:subcutaneous at neck(SC)and intramuscular at breast(IM)injection.The result showed that,no statistically significant difference was found between antibody titer of two administration routes as well as 2 vaccine doses until 13-week-old.Local reactions at the injection sites of IM route was less severe than SC at neck and in higher dose would produce a more severe swelling reaction.Daily weight gain was found to have a slight decrease in the vaccinated groups within 2 weeks after vaccination,however,no statistically significant difference was found in later stage(P>0.05).In conclusion,Ornitin Triple can be used to vaccinate by IM with the dose of 0.25ml for coloured broilers at early age(3-week-old),or 0.5ml for older birds and should be careful for some reactions at the injection sites.展开更多
文摘Liver transplantation (LT) is the best treatment for end-stage hepatic failure, with an excellent survival rates over the last decade. Biliary complications after LT pose a major challenge especially with the increasing number of procured organs after circulatory death. Ischaemic cholangiopathy (IC) is a set of disorders characterized by multiple diffuse strictures affecting the graft biliary system in the absence of hepatic artery thrombosis or stenosis. It commonly presents with cholestasis and cholangitis resulting in higher readmission rates, longer length of stay, repeated therapeutic interventions, and eventually re-transplantation with consequent effects on the patient’s quality of life and increased health care costs. The pathogenesis of IC is unclear and exhibits a higher prevalence with prolonged ischaemia time, donation after circulatory death (DCD), rejection, and cytomegalovirus infection. The majority of IC occurs within 12 mo after LT. Prolonged warm ischaemic times predispose to a profound injury with a subsequently higher prevalence of IC. Biliary complications and IC rates are between 16% and 29% in DCD grafts compared to between 3% and 17% in donation after brain death (DBD) grafts. The majority of ischaemic biliary lesions occur within 30 d in DCD compared to 90 d in DBD grafts following transplantation. However, there are many other risk factors for IC that should be considered. The benefits of DCD in expanding the donor pool are hindered by the higher incidence of IC with increased rates of re-transplantation. Careful donor selection and procurement might help to optimize the utilization of DCD grafts.
基金supported in part by the Ministry of Science and Technology(MoST),China under Grant No.2016YFE0127100 and 2017YFD0500705-5(HE Cheng)supported by the State Key Laboratory of Veterinary Etiological Biology,Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences+1 种基金Supported by Beijing Natural Science Foundation[No.6172019]partially funded by Taishan Scholar Project No.ts201511084
文摘Seasonal outbreaks of airsacculitis in China＇s poultry cause great economic losses annually. This study tried to unveil the potential role of Avian metopneumovirus （AM PV）, Ornithobacterium rhinotrocheale （aRT） and Chlarnydia psittaci （CPS） in avian airsacculitis. A serological investigation of 673 breeder chickens and a case-controlled study of 430 birds were undertaken. Results showed that infection with AMPV, aRT, and CPS was highly associated with the disease. The correlation between AMPV and CPS were positively robust in both layers and broilers. Finally, we determined the co-infection with AMPV, aRT, and CPS was prevalent in the sampled poultry farms suffering from respiratory diseases and the outbreak of airsacculitis was closely related to simultaneous exposure to all three agents.
文摘The study was to determine the appropriate dose and administration route of Ornitin Triple vaccine in cross-breed coloured broilers in Vietnam by evaluating the antibody titer against Ornithobacterium rhinotracheale(ORT)and local reactions at injection sites on chickens after vaccination.The study was divided into 2 trials.Both trials were designed with 3 vaccine dose groups:0.0ml(control group),0.25ml and 0.5ml and 2 different administration routes:subcutaneous at neck(SC)and intramuscular at breast(IM)injection.The result showed that,no statistically significant difference was found between antibody titer of two administration routes as well as 2 vaccine doses until 13-week-old.Local reactions at the injection sites of IM route was less severe than SC at neck and in higher dose would produce a more severe swelling reaction.Daily weight gain was found to have a slight decrease in the vaccinated groups within 2 weeks after vaccination,however,no statistically significant difference was found in later stage(P>0.05).In conclusion,Ornitin Triple can be used to vaccinate by IM with the dose of 0.25ml for coloured broilers at early age(3-week-old),or 0.5ml for older birds and should be careful for some reactions at the injection sites.
