Odorant-binding proteins （OBPs） are soluble proteins mediating chemorecep- tion in insects. In previous research, we investigated the molecular mechanisms adopted by aphids to detect the alarm pheromone （E）-fl-far...Odorant-binding proteins （OBPs） are soluble proteins mediating chemorecep- tion in insects. In previous research, we investigated the molecular mechanisms adopted by aphids to detect the alarm pheromone （E）-fl-farnesene and we found that the recogni- tion of this and structurally related molecules is mediated by OBP3 and OBP7. Here, we show the differential expression patterns of 5 selected OBPs （OBP 1, OBP3, OBP6, OBPT, OBPS） obtained performing quantitative RT-PCR and immunolocalization experiments in different body parts of adults and in the 5 developmental instars, including winged and unwinged morphs, of the pea aphid Acyrthosiphon pisum. The results provide an overall picture that allows us to speculate on the relationship between the differential expression of OBPs and their putative function. The expression of OBP3, OBP6, and OBP7 in the antennal sensilla suggests a ehemosensory fimction for these proteins, whereas the con- stant expression level of OBP8 in all instars could suggest a conserved role. Moreover, OBP1 and OBP3 are also expressed in nonsensory organs. A light and scanning electron microscopy study of sensilla on different body parts of aphid, in particular antennae, legs, mouthparts, and coruicles-cauda, completes this research providing a guide to facilitate the mapping of OBP expression profiles.展开更多
Trichogramma dendrolimi Matsumura （Hymenoptera： Trichogrammatidae） is one of the preponderant egg parasitoids of Dendrolimus spp., which are important defoliators of coniferous forests. This parasitoid wasp has bee...Trichogramma dendrolimi Matsumura （Hymenoptera： Trichogrammatidae） is one of the preponderant egg parasitoids of Dendrolimus spp., which are important defoliators of coniferous forests. This parasitoid wasp has been widely released to control pine caterpillar and other lepidopteran pests, but its control efficiency needs to be improved. Sensory systems are crucial for T dendrolimi to locate hosts, and immunity is probably involved after egg deposition in the host cavity; however, few reports have focused on the molecular mechanism of olfactory detection and survival of T. dendrolimi. It is necessary to identify these genes before further functional research is conducted. In this study, we assembled and analyzed the transcriptome of T. dendrolimi using next-generation sequencing technology. The sequencing and assembly resulted in 38 565 contigs with N50 of 3422 bp. Sequence comparison indicate that T dendrolimi sequences are very similar to those of another parasitoid Nasonia vitripennis. Then the olfactory, vision, and immune- related gene families were identified, and phylogenetic analyses were performed with these genes from T. dendrolimi and other model insect species. Furthermore, phylogenetic tree with odorant binding proteins of T. dendrolimi and their host Dendrolimus was constructed to determine whether convergent evolution exists. These genes can be valid targets for further gene function research. The present study may help us to understand host location and survival mechanisms of T dendrolimi and to use them more efficiently for pest control in the future.展开更多
We experimentally investigated the nonlinear optical response in few-layer oxidized black phosphorus(OBP) by the femtosecond Z-scan measurement technique, and found that OBP not only possesses strong ultrafast saturab...We experimentally investigated the nonlinear optical response in few-layer oxidized black phosphorus(OBP) by the femtosecond Z-scan measurement technique, and found that OBP not only possesses strong ultrafast saturable absorption but also a nonlinear self-defocusing effect that is absent in black phosphorus(BP). The saturable absorption property originates mainly from the direct band structure, which is still maintained in OBP. The emergence of self-defocusing might originate from the combined consequences of the oxygen-induced defects in BP. Our experimental findings might constitute the first experimental evidence on how to dynamically tune its nonlinear property, offering an inroad in tailoring its optical properties through chemical modification(oxidation, introducing defects, etc.). The versatile ultrafast nonlinear optical properties(saturable absorption and self-defocusing) imply a significant potential of the layered OBP in the development of unprecedented optoelectronic devices, such as mode lockers, optical switches, laser beam shapers, and wavelength converters.展开更多
Odorant-binding proteins (OBPs) play key roles in the perception of semiochemicals in insects. Several OBPs in insect olfactory systems have been functionally characterized, and they provide excellent targets for pest...Odorant-binding proteins (OBPs) play key roles in the perception of semiochemicals in insects. Several OBPs in insect olfactory systems have been functionally characterized, and they provide excellent targets for pest control. The functions of some OBPs that are highly expressed in the nonsensory organs of insects remain unclear. Here, the physiological function of an OBP (OBP27) that was highly expressed in the nonsensory organs of Spodoptera frugiperda was studied. OBP27 was nested within the Plus-C cluster according to phylogenetic analysis. The transcription of OBP27 steadily increased throughout the development of S. frugiperda, and transcripts of this gene were abundant in the fat body and male reproductive organs. An OBP27 knockout strain with an early frameshift mutation was obtained using the clustered regularly interspaced palindromic repeats (CRISPR) / CRISPR-associated protein 9 (Cas9) system. The development time of OBP27^(−/−) larvae was significantly longer than that of other larvae. Both male and female OBP27^(−/−) pupae weighed significantly less than wild-type (WT) pupae. In crosses of OBP27^(−/−) males or females, the mating rate was lower and the mating duration was longer for OBP27^(−/−) male–WT female pairs than for WT–WT pairs. By contrast, the mating rate, hatching rate, and number of eggs of OBP27^(−/−) female–WT male pairs and WT–WT pairs were similar. These findings indicate that OBP27 plays an important role in the larval development and mating process in male adults. Generally, our findings provide new insights into the physiological roles of nonsensory OBPs.展开更多
Odorant binding proteins(OBPs)are a group of soluble proteins functioning as odorant carriers in insect antennae,mouth parts and other chemosensory organs.However,multiple insect OBPs have been detected in other tissu...Odorant binding proteins(OBPs)are a group of soluble proteins functioning as odorant carriers in insect antennae,mouth parts and other chemosensory organs.However,multiple insect OBPs have been detected in other tissues and various functions have been proposed.Therefore,a detailed expression profile including stages,tissues and sexes where OBPs are expressed will assist in building the links to their potential functions,enhancing the functional studies of insect OBPs.Here,we identified 39 putative OBP genes from its genome and transcriptome sequences of diamondback moth(DBM),Plutella xylostella.The expression patterns of identified PxylOBPs were further investigated from eggs,larvae,pupae,virgin adults,mated adults,larval midgut,larval heads,adult antennae,adult heads and adult tarsi.Moreover,P.xylostella larvae and adults with and without host plants for 5 h were utilized to study the interactions between OBP expression and host plants.The results showed that most PxylOBPs were highly expressed in male and female adult antennae.The expression levels of certain PxyOBPs could be regulated by mating activities and feeding host plants.This study advances our knowledge of P.xylostella OBPs,which may help develop new strategies for more environmentally sustainable management of P.xylostella.展开更多
Cosmic radiation has several effects on the On-Board Processing(OBP)platform in satellite communications systems,and Single Event Upsets(SEUs)are one of its most important effects.In order to protect the Finite Impuls...Cosmic radiation has several effects on the On-Board Processing(OBP)platform in satellite communications systems,and Single Event Upsets(SEUs)are one of its most important effects.In order to protect the Finite Impulse Response(FIR)filters against SEU,this paper proposes a novel Residue Number(RN)-based method.The proposed method applies the transpose form of the FIR filter to avoid the fault missing caused by SEU on shift registers.It also adjusts the input intelligently to avoid the fault missing caused by SEU on the filter coefficients.After all the fault missing events are avoided,the modulus can be minimised to achieve the minimum overhead.Theoretical analysis and simulation results show that the noise introduced by the input adjustment is negligible.Fault injection shows that the fault missing rate of the proposed method is zero.Finally,FPGA implementation shows that the overhead of the proposed method is approximately 75% of Triple Modular Redundancy,and is only 1%-2% higher than that of the traditional RN-based design.展开更多
文摘Odorant-binding proteins （OBPs） are soluble proteins mediating chemorecep- tion in insects. In previous research, we investigated the molecular mechanisms adopted by aphids to detect the alarm pheromone （E）-fl-farnesene and we found that the recogni- tion of this and structurally related molecules is mediated by OBP3 and OBP7. Here, we show the differential expression patterns of 5 selected OBPs （OBP 1, OBP3, OBP6, OBPT, OBPS） obtained performing quantitative RT-PCR and immunolocalization experiments in different body parts of adults and in the 5 developmental instars, including winged and unwinged morphs, of the pea aphid Acyrthosiphon pisum. The results provide an overall picture that allows us to speculate on the relationship between the differential expression of OBPs and their putative function. The expression of OBP3, OBP6, and OBP7 in the antennal sensilla suggests a ehemosensory fimction for these proteins, whereas the con- stant expression level of OBP8 in all instars could suggest a conserved role. Moreover, OBP1 and OBP3 are also expressed in nonsensory organs. A light and scanning electron microscopy study of sensilla on different body parts of aphid, in particular antennae, legs, mouthparts, and coruicles-cauda, completes this research providing a guide to facilitate the mapping of OBP expression profiles.
基金Acknowledgments We express many thanks that Institute Special Fund for Basic Research, Institute of Forest Ecology, Environment, and Protection, Chinese Academy of Forestry （CAFRIFEEP201406, CAFRIFEEP201102-5） and the National Natural Science Foundation of China （31200492） supported this work. SFZ designed the experiments, carried out the laboratory work, analyzed the data and drafted the manuscript. ZZ designed the experiments and modified the manuscript. HBW and XBK modified the manuscript, and GZ and JXY reared and applied insects.
文摘Trichogramma dendrolimi Matsumura （Hymenoptera： Trichogrammatidae） is one of the preponderant egg parasitoids of Dendrolimus spp., which are important defoliators of coniferous forests. This parasitoid wasp has been widely released to control pine caterpillar and other lepidopteran pests, but its control efficiency needs to be improved. Sensory systems are crucial for T dendrolimi to locate hosts, and immunity is probably involved after egg deposition in the host cavity; however, few reports have focused on the molecular mechanism of olfactory detection and survival of T. dendrolimi. It is necessary to identify these genes before further functional research is conducted. In this study, we assembled and analyzed the transcriptome of T. dendrolimi using next-generation sequencing technology. The sequencing and assembly resulted in 38 565 contigs with N50 of 3422 bp. Sequence comparison indicate that T dendrolimi sequences are very similar to those of another parasitoid Nasonia vitripennis. Then the olfactory, vision, and immune- related gene families were identified, and phylogenetic analyses were performed with these genes from T. dendrolimi and other model insect species. Furthermore, phylogenetic tree with odorant binding proteins of T. dendrolimi and their host Dendrolimus was constructed to determine whether convergent evolution exists. These genes can be valid targets for further gene function research. The present study may help us to understand host location and survival mechanisms of T dendrolimi and to use them more efficiently for pest control in the future.
基金National Natural Science Foundation of China(NSFC)(61435010,61505117)Science and Technology Innovation Commission of Shenzhen(KQTD2015032416270385)+1 种基金China Postdoctoral Science Foundation(2015M580731)Scienceand Technology Planning Project of Guangdong Province(2016B050501005)
文摘We experimentally investigated the nonlinear optical response in few-layer oxidized black phosphorus(OBP) by the femtosecond Z-scan measurement technique, and found that OBP not only possesses strong ultrafast saturable absorption but also a nonlinear self-defocusing effect that is absent in black phosphorus(BP). The saturable absorption property originates mainly from the direct band structure, which is still maintained in OBP. The emergence of self-defocusing might originate from the combined consequences of the oxygen-induced defects in BP. Our experimental findings might constitute the first experimental evidence on how to dynamically tune its nonlinear property, offering an inroad in tailoring its optical properties through chemical modification(oxidation, introducing defects, etc.). The versatile ultrafast nonlinear optical properties(saturable absorption and self-defocusing) imply a significant potential of the layered OBP in the development of unprecedented optoelectronic devices, such as mode lockers, optical switches, laser beam shapers, and wavelength converters.
基金funded by the National Key R&D Program of China(2021YFD1400700)National Natural Science Foundation of China(31830075).
文摘Odorant-binding proteins (OBPs) play key roles in the perception of semiochemicals in insects. Several OBPs in insect olfactory systems have been functionally characterized, and they provide excellent targets for pest control. The functions of some OBPs that are highly expressed in the nonsensory organs of insects remain unclear. Here, the physiological function of an OBP (OBP27) that was highly expressed in the nonsensory organs of Spodoptera frugiperda was studied. OBP27 was nested within the Plus-C cluster according to phylogenetic analysis. The transcription of OBP27 steadily increased throughout the development of S. frugiperda, and transcripts of this gene were abundant in the fat body and male reproductive organs. An OBP27 knockout strain with an early frameshift mutation was obtained using the clustered regularly interspaced palindromic repeats (CRISPR) / CRISPR-associated protein 9 (Cas9) system. The development time of OBP27^(−/−) larvae was significantly longer than that of other larvae. Both male and female OBP27^(−/−) pupae weighed significantly less than wild-type (WT) pupae. In crosses of OBP27^(−/−) males or females, the mating rate was lower and the mating duration was longer for OBP27^(−/−) male–WT female pairs than for WT–WT pairs. By contrast, the mating rate, hatching rate, and number of eggs of OBP27^(−/−) female–WT male pairs and WT–WT pairs were similar. These findings indicate that OBP27 plays an important role in the larval development and mating process in male adults. Generally, our findings provide new insights into the physiological roles of nonsensory OBPs.
基金We are very grateful for the financial support from the National Natural Science Foundation of China(31320103922,31301666)Dr Wei Xu is the recipient of an Australian Research Council Discovery Early Career Researcher Award(DECRA)(DE160100382).
文摘Odorant binding proteins(OBPs)are a group of soluble proteins functioning as odorant carriers in insect antennae,mouth parts and other chemosensory organs.However,multiple insect OBPs have been detected in other tissues and various functions have been proposed.Therefore,a detailed expression profile including stages,tissues and sexes where OBPs are expressed will assist in building the links to their potential functions,enhancing the functional studies of insect OBPs.Here,we identified 39 putative OBP genes from its genome and transcriptome sequences of diamondback moth(DBM),Plutella xylostella.The expression patterns of identified PxylOBPs were further investigated from eggs,larvae,pupae,virgin adults,mated adults,larval midgut,larval heads,adult antennae,adult heads and adult tarsi.Moreover,P.xylostella larvae and adults with and without host plants for 5 h were utilized to study the interactions between OBP expression and host plants.The results showed that most PxylOBPs were highly expressed in male and female adult antennae.The expression levels of certain PxyOBPs could be regulated by mating activities and feeding host plants.This study advances our knowledge of P.xylostella OBPs,which may help develop new strategies for more environmentally sustainable management of P.xylostella.
基金supported by the National High Technical Research and Development Program of China (863 Program) "Research on the Key Technology for the Base Band Signal Processing for Onboard Payload"the Sino-Japan Joint Fund "Key Technique Research for GSS Integrated Mobile Satellite Communications"+2 种基金Tsinghua University Initiative Scientific Research Program "Key Technologies of SkyEarth Integration Wireless Communication Network" under Grant No. 2010 THZ03the National Key Basic Research Program of China(973 Program) under Grant No. 2012CB316000the Spanish Ministry of Science and Education under Grant No. AYA2009-13300-C03
文摘Cosmic radiation has several effects on the On-Board Processing(OBP)platform in satellite communications systems,and Single Event Upsets(SEUs)are one of its most important effects.In order to protect the Finite Impulse Response(FIR)filters against SEU,this paper proposes a novel Residue Number(RN)-based method.The proposed method applies the transpose form of the FIR filter to avoid the fault missing caused by SEU on shift registers.It also adjusts the input intelligently to avoid the fault missing caused by SEU on the filter coefficients.After all the fault missing events are avoided,the modulus can be minimised to achieve the minimum overhead.Theoretical analysis and simulation results show that the noise introduced by the input adjustment is negligible.Fault injection shows that the fault missing rate of the proposed method is zero.Finally,FPGA implementation shows that the overhead of the proposed method is approximately 75% of Triple Modular Redundancy,and is only 1%-2% higher than that of the traditional RN-based design.
