消费升级背景下传统电子商务服务体验不佳的弊端日渐显露,线上线下融合发展成为共识,但目前尚未形成有效实践模式。基于服务主导逻辑视角提出O2O(online to offline)电子商务模式内涵及价值创造过程模型,认为O2O电子商务模式是由内部和...消费升级背景下传统电子商务服务体验不佳的弊端日渐显露,线上线下融合发展成为共识,但目前尚未形成有效实践模式。基于服务主导逻辑视角提出O2O(online to offline)电子商务模式内涵及价值创造过程模型,认为O2O电子商务模式是由内部和外部、微观与宏观等不同类型利益相关者构成的服务生态系统,包括价值主张、资源整合、服务交互和环境适应等基本价值创造过程,以及线上服务平台和线下服务网点两类服务交互界面,并讨论模型应用过程中应注意的关键环节,为相关企业的O2O电子商务转型实践提供参考。展开更多
Hydrogen peroxide(H2O2),as a signaling molecule,plays a vital role in a wide variety of signaling transduction processes,aging,and diseases.However,the excessive production of H2O2 causes various diseases.Herein,we de...Hydrogen peroxide(H2O2),as a signaling molecule,plays a vital role in a wide variety of signaling transduction processes,aging,and diseases.However,the excessive production of H2O2 causes various diseases.Herein,we develop a novel method for H2O2 detection in live cells via dark-field scattering spectroscopy with gold triangular nanoprisms(AuTNPs)as probes.The corners of AuTNPs would be gradually oxidatively etched by the strong coordination of Br•which is generated by enzymatic reactions in the presence of horseradish peroxidase(HRP),bromide ion and trace hydrogen peroxide.Benefitting from the morphological change,the single AuTNP based plasmonic nanoprobe shows notable blueshifts and scattering color changes which could be real-time monitored under the dark-field microscopy.The peak position in the scattering spectra of individual AuTNP blueshifts linearly with the increase of H2O2 concentration,and exhibits high sensitivity to H2O2 in a large range from 2.5 to 100µM with a low detection limit(LOD)of 0.74µM.Moreover,the experimental results were supported by the simulated results via the finite-difference time-domain(FDTD)method.The nanoprobes have been further used for intracellular H2O2 detection in live cells.Besides,the etching of AuTNP also provides an alternative method to design novel plasmonic logic chips and write-once plasmonic memories.展开更多
文摘消费升级背景下传统电子商务服务体验不佳的弊端日渐显露,线上线下融合发展成为共识,但目前尚未形成有效实践模式。基于服务主导逻辑视角提出O2O(online to offline)电子商务模式内涵及价值创造过程模型,认为O2O电子商务模式是由内部和外部、微观与宏观等不同类型利益相关者构成的服务生态系统,包括价值主张、资源整合、服务交互和环境适应等基本价值创造过程,以及线上服务平台和线下服务网点两类服务交互界面,并讨论模型应用过程中应注意的关键环节,为相关企业的O2O电子商务转型实践提供参考。
基金This work was financially supported by the National Key Research and Development Program of China(No.2017YFA0205302)the National Natural Science Foundation of China(Nos.61571239 and 21674048)+1 种基金Program for Changjiang Scholars and Innovative Research Team in University(No.IRT_15R37)the Key Research and Development Program of Jiangsu(No.BE2018732).
文摘Hydrogen peroxide(H2O2),as a signaling molecule,plays a vital role in a wide variety of signaling transduction processes,aging,and diseases.However,the excessive production of H2O2 causes various diseases.Herein,we develop a novel method for H2O2 detection in live cells via dark-field scattering spectroscopy with gold triangular nanoprisms(AuTNPs)as probes.The corners of AuTNPs would be gradually oxidatively etched by the strong coordination of Br•which is generated by enzymatic reactions in the presence of horseradish peroxidase(HRP),bromide ion and trace hydrogen peroxide.Benefitting from the morphological change,the single AuTNP based plasmonic nanoprobe shows notable blueshifts and scattering color changes which could be real-time monitored under the dark-field microscopy.The peak position in the scattering spectra of individual AuTNP blueshifts linearly with the increase of H2O2 concentration,and exhibits high sensitivity to H2O2 in a large range from 2.5 to 100µM with a low detection limit(LOD)of 0.74µM.Moreover,the experimental results were supported by the simulated results via the finite-difference time-domain(FDTD)method.The nanoprobes have been further used for intracellular H2O2 detection in live cells.Besides,the etching of AuTNP also provides an alternative method to design novel plasmonic logic chips and write-once plasmonic memories.
