AIM To study the expression of P21 and CEA, and AgNOR counts in various lesions of colonic mucosa and the mechanism of carcinogenesis. METHODS Thirty eight male Wistar rats were injected with DMH once a week, the ...AIM To study the expression of P21 and CEA, and AgNOR counts in various lesions of colonic mucosa and the mechanism of carcinogenesis. METHODS Thirty eight male Wistar rats were injected with DMH once a week, the experiment lasted 25 weeks. The expression of P21 and CEA was detected by the immunohistochemical methods and AgNOR was counted with silver staining in paraffin sections of various colonic lesions. RESULTS The incidence of colonic carcinoma in DMH treated rats was 71 05%(27/38), and lymphnode metastasis occurred in 6 rats. Immunohistochemical study showed that the expression of P21 was mainly in dysplasia and carcinomas, while CEA expressed in carcinoma and metastasis. The AgNOR counts was also increased in dysplasia and carcinomas. There were significant differences in P21 and CEA expression between benign and malignant lesions ( P <0 05). Difference in AgNOR counts was also significant between normal and dysplasia, dysplasia and malignant lesions ( P <0 05). CONCLUSION Dysplasia is a premalignant change of colonic carcinoma. The detection of P21 with immunohistochemistry and AgNOR counting might be important for screening colonic carcinoma and premalignant lesions clinically.展开更多
The number of AgNORs in the nucleolar organizer region was studied quantitativelyby one-step silver colloid technique in 6 samples obtained from normal bladder tissue,9 ofchronic cystitis and 56 of transitional cell c...The number of AgNORs in the nucleolar organizer region was studied quantitativelyby one-step silver colloid technique in 6 samples obtained from normal bladder tissue,9 ofchronic cystitis and 56 of transitional cell carcinoma of bladder.The results revealed that meannumber of AgNORs per nucleus was not statistically different between normal bladder tissueand tissue from chronic cystitis,while there was a significant difference between normal or in-flammatory tissue and malignant tisssue on the one hand and between various grades of bladdertumor on the other.The results also indicated that mean number of AgNORs per nucleus wasclosely related to the prognosis of bladder tumor.So it is suggested that AgNOR counting bythe silver stain technique might play a role in the differenciation of benign and malignant lesionsand in grading and prognosis in bladder tumors.展开更多
文摘AIM To study the expression of P21 and CEA, and AgNOR counts in various lesions of colonic mucosa and the mechanism of carcinogenesis. METHODS Thirty eight male Wistar rats were injected with DMH once a week, the experiment lasted 25 weeks. The expression of P21 and CEA was detected by the immunohistochemical methods and AgNOR was counted with silver staining in paraffin sections of various colonic lesions. RESULTS The incidence of colonic carcinoma in DMH treated rats was 71 05%(27/38), and lymphnode metastasis occurred in 6 rats. Immunohistochemical study showed that the expression of P21 was mainly in dysplasia and carcinomas, while CEA expressed in carcinoma and metastasis. The AgNOR counts was also increased in dysplasia and carcinomas. There were significant differences in P21 and CEA expression between benign and malignant lesions ( P <0 05). Difference in AgNOR counts was also significant between normal and dysplasia, dysplasia and malignant lesions ( P <0 05). CONCLUSION Dysplasia is a premalignant change of colonic carcinoma. The detection of P21 with immunohistochemistry and AgNOR counting might be important for screening colonic carcinoma and premalignant lesions clinically.
文摘The number of AgNORs in the nucleolar organizer region was studied quantitativelyby one-step silver colloid technique in 6 samples obtained from normal bladder tissue,9 ofchronic cystitis and 56 of transitional cell carcinoma of bladder.The results revealed that meannumber of AgNORs per nucleus was not statistically different between normal bladder tissueand tissue from chronic cystitis,while there was a significant difference between normal or in-flammatory tissue and malignant tisssue on the one hand and between various grades of bladdertumor on the other.The results also indicated that mean number of AgNORs per nucleus wasclosely related to the prognosis of bladder tumor.So it is suggested that AgNOR counting bythe silver stain technique might play a role in the differenciation of benign and malignant lesionsand in grading and prognosis in bladder tumors.
