[Objective] It is to clone broad-spectrum anti-disease gene NPR1 and to construct its protein expression vector.[Method] First to extract total RNA of Arabidopsis thaliana and design relevant primers,and then the meth...[Objective] It is to clone broad-spectrum anti-disease gene NPR1 and to construct its protein expression vector.[Method] First to extract total RNA of Arabidopsis thaliana and design relevant primers,and then the method of reverse transcription PCR was adopted to clone.With the method of enzyme digestion and ligation,this gene will be directed into protein expression vector.[Result] After relevant testing,NPR1 was inserted into vector pMXB10 to obtain pMXB10-NPR1 protein expression vector.[Conclusion] Protein expression vector including NPR1 was successfully constructed.展开更多
NPR(Nonexpressor of pathogenesis-related genes)家族蛋白为植物水杨酸(SA)受体,在细胞核内作为共转录因子参与植物多种生理过程。NPR蛋白功能多样性的发挥依赖于SA诱导下空间结构的变化,NPR空间结构变化为其功能发挥奠定结构基础。...NPR(Nonexpressor of pathogenesis-related genes)家族蛋白为植物水杨酸(SA)受体,在细胞核内作为共转录因子参与植物多种生理过程。NPR蛋白功能多样性的发挥依赖于SA诱导下空间结构的变化,NPR空间结构变化为其功能发挥奠定结构基础。通过综述NPR家族蛋白在植物系统获得性抗性(SAR)、形态发育、冷胁迫、昼夜节律中发挥生物学功能以及NPR1、NPR1-TGA3(TGACG sequence-specific binding protein)复合物、NPR4空间结构的最新研究进展,为解决植物在胁迫条件下的培养问题提供新思路。展开更多
基金Supported by project of Beijing Municipal Education Commission(KM200910020014)Project of Sand Control Department,Beijing Municipal Landscape Greening Bureau(2008)~~
文摘[Objective] It is to clone broad-spectrum anti-disease gene NPR1 and to construct its protein expression vector.[Method] First to extract total RNA of Arabidopsis thaliana and design relevant primers,and then the method of reverse transcription PCR was adopted to clone.With the method of enzyme digestion and ligation,this gene will be directed into protein expression vector.[Result] After relevant testing,NPR1 was inserted into vector pMXB10 to obtain pMXB10-NPR1 protein expression vector.[Conclusion] Protein expression vector including NPR1 was successfully constructed.
