Due to the innate extracellular matrix mimicking features,fibrous materials exhibited great application potential in biomedicine.In developing excellent fibrous biomaterial,it is essential to reveal the corresponding ...Due to the innate extracellular matrix mimicking features,fibrous materials exhibited great application potential in biomedicine.In developing excellent fibrous biomaterial,it is essential to reveal the corresponding inherent fiber features’effects on cell behaviors.Due to the inevitable‘interference’cell adhesions to the background or between adjacent fibers,it is difficult to precisely reveal the inherent fiber diameter effect on cell behaviors by using a traditional fiber mat.A single-layer and parallel-arranged polycaprolactone fiber pattern platform with an excellent non-fouling background is designed and constructed herein.In this unique material platform,the‘interference’cell adhesions through interspace between fibers to the environment could be effectively ruled out by the non-fouling background.The‘interference’cell adhesions between adjacent fibers could also be excluded from the sparsely arranged(SA)fiber patterns.The influence of fiber diameter on stem cell behaviors is precisely and comprehensively investigated based on eliminating the undesired‘interference’cell adhesions in a controllable way.On the SA fiber patterns,small diameter fiber(SA-D1,D1 means 1μm in diameter)may seriously restrict cell proliferation and osteogenesis when compared to the middle(SA-D8)and large(SA-D56)ones and SA-D8 shows the optimal osteogenesis enhancement effect.At the same time,the cells present similar proliferation ability and even the highest osteogenic ability on the densely arranged(DA)fiber patterns with small diameter fiber(DA-D1)when compared to the middle(DA-D8)and large(DA-D56)ones.The‘interference’cell adhesion between adjacent fibers under dense fiber arrangement may be the main reason for inducing these different cell behavior trends along with fiber diameters.Related results and comparisons have illustrated the effects of fiber diameter on stem cell behaviors more precisely and objectively,thus providing valuable reference and guidance for developing effective fibrous biomaterials.展开更多
Bacteriorhodopsin is a well-known photoresponsive protein in the cell membrane of Halobacterium salinarum and exists in the patch called the purple membrane(PM).This letter reports,for the first time,its new function ...Bacteriorhodopsin is a well-known photoresponsive protein in the cell membrane of Halobacterium salinarum and exists in the patch called the purple membrane(PM).This letter reports,for the first time,its new function as a natural non-fouling substance for resisting cell adhesion.Mammalian cells such as murine preosteocytes MC3T3-E1 were seeded on the PM film.A significant resistance to cell adhesion on the film was found.展开更多
Zwitterionic materials are now widely used to fabricate various functionalized surfaces for biomedical applications due to their excellent non-fouling properties.However,a newly-discovered zwitterionic material,cholin...Zwitterionic materials are now widely used to fabricate various functionalized surfaces for biomedical applications due to their excellent non-fouling properties.However,a newly-discovered zwitterionic material,choline phosphate(CP),was reported to be cell-adhesive,which makes it different from traditional non-fouling zwitterionic materials such as sulfobetaine,carboxybetaine and phosphorylcholine(PC).To further investigate the properties of CP,a comparative study was conducted and the widely-reported zwitterionic PC was employed as a control which has the same chemical component but opposite orientation of charged groups with CP.For this purpose,CP and PC-functionalized surfaces were prepared by surface-initiated atom transfer radical polymerization(Si-ATRP),and their non-fouling properties were probed by protein adsorption and eukaryotic cell adhesion measurement.Results showed that CP-functionalized surfaces exhibited almost equivalent amounts of adsorbed proteins to that of PC,but they were more beneficial to cells initial adhesion and further spreading in serum-free medium,indicating that CP had a promising prospect of application in tissue engineering.展开更多
基金sponsored by the Natural Science Foundation of Shanghai(20ZR1402400)the Fundamental Research Funds for the Central Universities(2232020D-04)+2 种基金the National Natural Science Foundation of China(52273125,52173031 and 51903045)the Science and Technology Commission of Shanghai Municipality(20XD1400100,22520711900 and 21JC1400100)the Fundamental Research Funds for the Central Universities(2232019A3-06 and 2232019D3-02).
文摘Due to the innate extracellular matrix mimicking features,fibrous materials exhibited great application potential in biomedicine.In developing excellent fibrous biomaterial,it is essential to reveal the corresponding inherent fiber features’effects on cell behaviors.Due to the inevitable‘interference’cell adhesions to the background or between adjacent fibers,it is difficult to precisely reveal the inherent fiber diameter effect on cell behaviors by using a traditional fiber mat.A single-layer and parallel-arranged polycaprolactone fiber pattern platform with an excellent non-fouling background is designed and constructed herein.In this unique material platform,the‘interference’cell adhesions through interspace between fibers to the environment could be effectively ruled out by the non-fouling background.The‘interference’cell adhesions between adjacent fibers could also be excluded from the sparsely arranged(SA)fiber patterns.The influence of fiber diameter on stem cell behaviors is precisely and comprehensively investigated based on eliminating the undesired‘interference’cell adhesions in a controllable way.On the SA fiber patterns,small diameter fiber(SA-D1,D1 means 1μm in diameter)may seriously restrict cell proliferation and osteogenesis when compared to the middle(SA-D8)and large(SA-D56)ones and SA-D8 shows the optimal osteogenesis enhancement effect.At the same time,the cells present similar proliferation ability and even the highest osteogenic ability on the densely arranged(DA)fiber patterns with small diameter fiber(DA-D1)when compared to the middle(DA-D8)and large(DA-D56)ones.The‘interference’cell adhesion between adjacent fibers under dense fiber arrangement may be the main reason for inducing these different cell behavior trends along with fiber diameters.Related results and comparisons have illustrated the effects of fiber diameter on stem cell behaviors more precisely and objectively,thus providing valuable reference and guidance for developing effective fibrous biomaterials.
基金supported by the National Basic Research Program of China (2009CB930000, 2011CB606203)the National Natural Science Foundation of China (21034002)
文摘Bacteriorhodopsin is a well-known photoresponsive protein in the cell membrane of Halobacterium salinarum and exists in the patch called the purple membrane(PM).This letter reports,for the first time,its new function as a natural non-fouling substance for resisting cell adhesion.Mammalian cells such as murine preosteocytes MC3T3-E1 were seeded on the PM film.A significant resistance to cell adhesion on the film was found.
基金support by the National Natural Science Foundation of China(51503126,21534008,51573110)Scientific Research Foundation for Young Teachers of Sichuan University(2015SCU11005)Miaozi Project in Science and Technology Innovation Program of Sichuan Province(2016087).
文摘Zwitterionic materials are now widely used to fabricate various functionalized surfaces for biomedical applications due to their excellent non-fouling properties.However,a newly-discovered zwitterionic material,choline phosphate(CP),was reported to be cell-adhesive,which makes it different from traditional non-fouling zwitterionic materials such as sulfobetaine,carboxybetaine and phosphorylcholine(PC).To further investigate the properties of CP,a comparative study was conducted and the widely-reported zwitterionic PC was employed as a control which has the same chemical component but opposite orientation of charged groups with CP.For this purpose,CP and PC-functionalized surfaces were prepared by surface-initiated atom transfer radical polymerization(Si-ATRP),and their non-fouling properties were probed by protein adsorption and eukaryotic cell adhesion measurement.Results showed that CP-functionalized surfaces exhibited almost equivalent amounts of adsorbed proteins to that of PC,but they were more beneficial to cells initial adhesion and further spreading in serum-free medium,indicating that CP had a promising prospect of application in tissue engineering.
