Objective:To isolate and identify Nocardiaspp. from soil in different regions of Isfahan province in the center of Iran.Methods:This study was conducted in 32 districts(16 cities and 16 villages)in Isfahan province du...Objective:To isolate and identify Nocardiaspp. from soil in different regions of Isfahan province in the center of Iran.Methods:This study was conducted in 32 districts(16 cities and 16 villages)in Isfahan province during two years. A total of 800 soil samples from these regions were studied by using kanamycin. The isolated Nocardiaspecies were examined by gram and acid-fast staining and were identified biochemically and morphologically. The frequency and distribution of Nocardia spp. were determined in relation to different factors such as soil pH and temperate climate.Results:From 153(19.1%)Nocardiaisolates identified,Nocardia asteroids(N. asteroids)complex(45.5%) andNocardia brasiliensis(N. brasiliensis)(24.7%) were the most frequently isolated species, followed by Nocardia otitidiscaviarum (2.2%),Nocardiopsis dassonvillei,Actinom adura actinomadura(each 1.7%) and Nocardia transvalensis(1.1%) and also unknown spp.(23.0%). In this study, most species(54.4%) of Nocardia, especially N. asteroides complex were isolated fromsoils with pH: 7.01-8, whereas in pH: 8.01-9 more N. brasiliensis was isolated. The most Nocardia spp. was detected from regions with semi-nomadic and temperate climate(41.1%).Conclusions:N. asteroids complex is more prevalent in Isfahan province and soil can be a potential source of nocardiosis infections. It is to be considering that climate and soil pH are involved in the frequency and diversity of aerobic Actinomy cetes.展开更多
基金financially supported by Teheran University of Medical Sciences(grant No.TUMS/HF-2446)
文摘Objective:To isolate and identify Nocardiaspp. from soil in different regions of Isfahan province in the center of Iran.Methods:This study was conducted in 32 districts(16 cities and 16 villages)in Isfahan province during two years. A total of 800 soil samples from these regions were studied by using kanamycin. The isolated Nocardiaspecies were examined by gram and acid-fast staining and were identified biochemically and morphologically. The frequency and distribution of Nocardia spp. were determined in relation to different factors such as soil pH and temperate climate.Results:From 153(19.1%)Nocardiaisolates identified,Nocardia asteroids(N. asteroids)complex(45.5%) andNocardia brasiliensis(N. brasiliensis)(24.7%) were the most frequently isolated species, followed by Nocardia otitidiscaviarum (2.2%),Nocardiopsis dassonvillei,Actinom adura actinomadura(each 1.7%) and Nocardia transvalensis(1.1%) and also unknown spp.(23.0%). In this study, most species(54.4%) of Nocardia, especially N. asteroides complex were isolated fromsoils with pH: 7.01-8, whereas in pH: 8.01-9 more N. brasiliensis was isolated. The most Nocardia spp. was detected from regions with semi-nomadic and temperate climate(41.1%).Conclusions:N. asteroids complex is more prevalent in Isfahan province and soil can be a potential source of nocardiosis infections. It is to be considering that climate and soil pH are involved in the frequency and diversity of aerobic Actinomy cetes.
