Flower blight on anthurium(Anthurium andraeanum)was observed during August 2018 on an anthurium cultivation farm in the Songkhla Province of southern Thailand.The fungal isolate was identified as Neopestalotiopsis cla...Flower blight on anthurium(Anthurium andraeanum)was observed during August 2018 on an anthurium cultivation farm in the Songkhla Province of southern Thailand.The fungal isolate was identified as Neopestalotiopsis clavispora based on the morphology and DNA sequence of the internal transcribed spacer(ITS),translation elongation factor 1-α(tef1-α),andβ-tubulin(tub)genes.The phylogenetic tree,based on the combined sequences of ITS,tef1-α,and tub,confirmed this pathogen as N.clavispora.Pathogenicity of the species was confirmed according to Koch’s postulate:N.clavispora could infect anthurium.To the best of our knowledge,this is the first report of N.clavispora as a pathogen of anthurium.展开更多
[Objectives]The study was to confirm the etiological agent of leaf spot on banana in Yunnan Province of China.[Methods]Fungus isolates were isolated from the diseased tissues,and cultured to observe the morphological ...[Objectives]The study was to confirm the etiological agent of leaf spot on banana in Yunnan Province of China.[Methods]Fungus isolates were isolated from the diseased tissues,and cultured to observe the morphological characteristics of colony and spore.Furthermore,phylogenetic analyses and pathogenicity test were also conducted to confirm the pathogen.[Results]The fungus isolated from the diseased tissues was identified as Neopestalotiopsis clavispora.[Conclusions]N.clavispora is a new pathogen causing leaf spot of banana.This research provides the first description of N.clavispora as a causal agent on banana in China,and adds new insights related to the host range of N.clavispora.展开更多
Grapevine(Vitis vinifera L.)is an economically important fruit crop in the world,and China ranks first in the production of grapes with approximately 15%of the world’s total yield.However,diseases that cause the deat...Grapevine(Vitis vinifera L.)is an economically important fruit crop in the world,and China ranks first in the production of grapes with approximately 15%of the world’s total yield.However,diseases that cause the death of grapevine shoots pose a severe threat to the production of grapes.In this study,the fungus Neopestalotiopsis eucalypti was identified as a causal pathogen of grapevine shoot rot based on the morphology of conidia and a phylogenetic analysis.The phylogenetic analysis was performed with three isolates based on the combined sequence of internal transcribed spacer(ITS)region of ribosomal DNA,part of the translation elongation factor 1-alpha(Tef)and theβ-tubulin(Tub2)genes.The three isolates were all identified as N.eucalypti.Pathogenicity tests of the three fungal isolates were conducted on grapevines shoots in vitro and in vivo.The results showed that all three fungal isolates caused severe rot lesions on the inoculated grapevine shoots,and N.eucalypti was re-isolated from the inoculated grapevine shoots.Therefore,N.eucalypti was confirmed as a causal agent of the grapevine shoot rot.This is the first report of N.eucalypti causing grapevine shoot disease in China.展开更多
基金supported by Prince of Songkla Universitythe Center of Excellence in Agricultural and Natural Resources Biotechnology(Grant No.CoE-ANRB)phase 3。
文摘Flower blight on anthurium(Anthurium andraeanum)was observed during August 2018 on an anthurium cultivation farm in the Songkhla Province of southern Thailand.The fungal isolate was identified as Neopestalotiopsis clavispora based on the morphology and DNA sequence of the internal transcribed spacer(ITS),translation elongation factor 1-α(tef1-α),andβ-tubulin(tub)genes.The phylogenetic tree,based on the combined sequences of ITS,tef1-α,and tub,confirmed this pathogen as N.clavispora.Pathogenicity of the species was confirmed according to Koch’s postulate:N.clavispora could infect anthurium.To the best of our knowledge,this is the first report of N.clavispora as a pathogen of anthurium.
基金Supported by High level Talents Project of Hainan Provincial Natural Science Foundation of China(2019RC278)The Earmarked Fund for CARS(CARS-31).
文摘[Objectives]The study was to confirm the etiological agent of leaf spot on banana in Yunnan Province of China.[Methods]Fungus isolates were isolated from the diseased tissues,and cultured to observe the morphological characteristics of colony and spore.Furthermore,phylogenetic analyses and pathogenicity test were also conducted to confirm the pathogen.[Results]The fungus isolated from the diseased tissues was identified as Neopestalotiopsis clavispora.[Conclusions]N.clavispora is a new pathogen causing leaf spot of banana.This research provides the first description of N.clavispora as a causal agent on banana in China,and adds new insights related to the host range of N.clavispora.
基金the financial support from the earmarked fund for China Agriculture Research System(CARS-27)
文摘Grapevine(Vitis vinifera L.)is an economically important fruit crop in the world,and China ranks first in the production of grapes with approximately 15%of the world’s total yield.However,diseases that cause the death of grapevine shoots pose a severe threat to the production of grapes.In this study,the fungus Neopestalotiopsis eucalypti was identified as a causal pathogen of grapevine shoot rot based on the morphology of conidia and a phylogenetic analysis.The phylogenetic analysis was performed with three isolates based on the combined sequence of internal transcribed spacer(ITS)region of ribosomal DNA,part of the translation elongation factor 1-alpha(Tef)and theβ-tubulin(Tub2)genes.The three isolates were all identified as N.eucalypti.Pathogenicity tests of the three fungal isolates were conducted on grapevines shoots in vitro and in vivo.The results showed that all three fungal isolates caused severe rot lesions on the inoculated grapevine shoots,and N.eucalypti was re-isolated from the inoculated grapevine shoots.Therefore,N.eucalypti was confirmed as a causal agent of the grapevine shoot rot.This is the first report of N.eucalypti causing grapevine shoot disease in China.
