Objective： To investigate the effects of electroacupuncture （EA） at Neiguan （PC 6） and Baihui （GV 20） by observing the changes of CCAAT/enhancer-binding protein（C/EBP） homologous protein （CHOP） and caspase-...Objective： To investigate the effects of electroacupuncture （EA） at Neiguan （PC 6） and Baihui （GV 20） by observing the changes of CCAAT/enhancer-binding protein（C/EBP） homologous protein （CHOP） and caspase-12 gene expressions in rats after cerebral ischemia-reperfusion injury （IRI）, and explore whether the apoptosis pathway of endoplasmic reticulum stress （ERS） is involved in the protective mechanisms of EA. Methods： Sixty rats were randomly assigned to five groups （12 in each group）： a normal control group （group A）, a sham-operation group （group B）, an operation group （group C）, an Edaravone group （group D） and an EA group （group E）. The cerebral IRI rat model was induced by middle cerebral artery occlusion （MCAO） using intraluminal monofilament. 2,3,5-triphenyl tetrazolium chloride （TTC） staining was adopted in the measurement of cerebral infarction volume. Real-time polymerase chain reaction （RT-PCR） was used to determine the mRNA expressions of CHOP and caspase-12. Results： Compared with group A and group B, the volume of cerebral infarction and mRNA expressions of CHOP and caspase-12 in group C, group D and group E were increased, with statistical significances （P〈0.05 or P〈O.01）; compared with group C, the volume of cerebral infarction and mRNA expressions of CHOP and caspase-12 in group D and group E were decreased significantly （P〈0.05 or P〈0.01）; there were no significant differences between group D and group E in comparing the above items （P〉0.05）. Conclusion： EA at Neiguan （PC 6） and Baihui （GV 20） can effectively suppress the volume of cerebral infarction. Furthermore, the underlying mechanism of EA at Neiguan （PC 6） and Baihui （GV 20） is possibly related to the down-regulation of CHOP and caspase-12 mRNA expressions, so as to decrease cell apoptosis.展开更多
[目的]系统评价内关穴按压对缓解癌症病人化疗引起恶心、呕吐症状的临床疗效。[方法]计算机检索Medline、EMbase、Cochrane Library、CINAHL、Web of Science、中国生物医学数据库、万方数据库、中国知网和重庆维普中文科技期刊数据库,...[目的]系统评价内关穴按压对缓解癌症病人化疗引起恶心、呕吐症状的临床疗效。[方法]计算机检索Medline、EMbase、Cochrane Library、CINAHL、Web of Science、中国生物医学数据库、万方数据库、中国知网和重庆维普中文科技期刊数据库,检索时间为建库至2018年12月4日,收集内关穴按压缓解癌症病人化疗引起恶心、呕吐症状的随机对照试验研究,采用Revman5.3软件进行统计学分析。[结果]共纳入11篇文献,涉及癌症化疗病人606例;Meta分析结果显示,与对照组相比,试验组内关穴按压能有效改善癌症病人化疗引起的恶心症状的严重程度[MD=-1.75,95%CI(-2.95,-0.55),P=0.004]、呕吐症状严重程度[MD=-1.10,95%CI(-1.87,-0.33),P=0.005]、恶心症状发生频率[MD=-0.84,95%CI(-1.37,-0.31),P=0.002]、呕吐症状发生频率[MD=-0.64,95%CI(-1.24,-0.05),P=0.03]和干呕发生频率[MD=-0.31,95%CI(-0.53,-0.09),P=0.006];而在改善干呕症状的严重程度[MD=-0.40,95%CI(-0.90,0.10),P=0.12]和呕吐症状经历时间[MD=-0.52,95%CI(-1.21,0.17),P=0.14]方面效果不明显。[结论]当前证据表明,内关穴按压能改善癌症病人化疗引起恶心、呕吐症状严重程度,降低恶心、呕吐和干呕的发生频率,但在改善干呕严重程度和呕吐经历时间方面效果不明显。展开更多
基金supported by National Natural Science Foundation of China,No.81202771~~
文摘Objective： To investigate the effects of electroacupuncture （EA） at Neiguan （PC 6） and Baihui （GV 20） by observing the changes of CCAAT/enhancer-binding protein（C/EBP） homologous protein （CHOP） and caspase-12 gene expressions in rats after cerebral ischemia-reperfusion injury （IRI）, and explore whether the apoptosis pathway of endoplasmic reticulum stress （ERS） is involved in the protective mechanisms of EA. Methods： Sixty rats were randomly assigned to five groups （12 in each group）： a normal control group （group A）, a sham-operation group （group B）, an operation group （group C）, an Edaravone group （group D） and an EA group （group E）. The cerebral IRI rat model was induced by middle cerebral artery occlusion （MCAO） using intraluminal monofilament. 2,3,5-triphenyl tetrazolium chloride （TTC） staining was adopted in the measurement of cerebral infarction volume. Real-time polymerase chain reaction （RT-PCR） was used to determine the mRNA expressions of CHOP and caspase-12. Results： Compared with group A and group B, the volume of cerebral infarction and mRNA expressions of CHOP and caspase-12 in group C, group D and group E were increased, with statistical significances （P〈0.05 or P〈O.01）; compared with group C, the volume of cerebral infarction and mRNA expressions of CHOP and caspase-12 in group D and group E were decreased significantly （P〈0.05 or P〈0.01）; there were no significant differences between group D and group E in comparing the above items （P〉0.05）. Conclusion： EA at Neiguan （PC 6） and Baihui （GV 20） can effectively suppress the volume of cerebral infarction. Furthermore, the underlying mechanism of EA at Neiguan （PC 6） and Baihui （GV 20） is possibly related to the down-regulation of CHOP and caspase-12 mRNA expressions, so as to decrease cell apoptosis.
文摘[目的]系统评价内关穴按压对缓解癌症病人化疗引起恶心、呕吐症状的临床疗效。[方法]计算机检索Medline、EMbase、Cochrane Library、CINAHL、Web of Science、中国生物医学数据库、万方数据库、中国知网和重庆维普中文科技期刊数据库,检索时间为建库至2018年12月4日,收集内关穴按压缓解癌症病人化疗引起恶心、呕吐症状的随机对照试验研究,采用Revman5.3软件进行统计学分析。[结果]共纳入11篇文献,涉及癌症化疗病人606例;Meta分析结果显示,与对照组相比,试验组内关穴按压能有效改善癌症病人化疗引起的恶心症状的严重程度[MD=-1.75,95%CI(-2.95,-0.55),P=0.004]、呕吐症状严重程度[MD=-1.10,95%CI(-1.87,-0.33),P=0.005]、恶心症状发生频率[MD=-0.84,95%CI(-1.37,-0.31),P=0.002]、呕吐症状发生频率[MD=-0.64,95%CI(-1.24,-0.05),P=0.03]和干呕发生频率[MD=-0.31,95%CI(-0.53,-0.09),P=0.006];而在改善干呕症状的严重程度[MD=-0.40,95%CI(-0.90,0.10),P=0.12]和呕吐症状经历时间[MD=-0.52,95%CI(-1.21,0.17),P=0.14]方面效果不明显。[结论]当前证据表明,内关穴按压能改善癌症病人化疗引起恶心、呕吐症状严重程度,降低恶心、呕吐和干呕的发生频率,但在改善干呕严重程度和呕吐经历时间方面效果不明显。
