Electro-spinning is a very modern process which can be used in various purposes. We did this experimental work at Swerea IVF in Sweden during M. Sc in Textile Technology programme at University of Bor?s. We should esp...Electro-spinning is a very modern process which can be used in various purposes. We did this experimental work at Swerea IVF in Sweden during M. Sc in Textile Technology programme at University of Bor?s. We should especially thank our supervisor—Anna Thorvaldsson and course teacher—Ioannis S. Chronakis. In this report, we have tried to explain the basic manufacturing techniques of the electrospun nanofiber by the electro-spinning, how one can characterize it by SEM (Scanning Electron Microscopy) and its various applications in the practical field, e.g wound healing, Tissue Engineering Scaffold. The experimental work helped us a lot to gather sufficient knowledge about the electro-spinning process which we wanted to share with all.展开更多
目的检测重组人骨形成蛋白2(recombinant human bone morphogenetic protein2,rhBMP-2)与纳米羟基磷灰石/胶原/聚乳酸(nano-hydroxyapatite/collagen/polylactic acid,nHAC/PLA)复合后形成的活性nHAC/PLA(active nHAC/PLA,AnHAC/PLA)修...目的检测重组人骨形成蛋白2(recombinant human bone morphogenetic protein2,rhBMP-2)与纳米羟基磷灰石/胶原/聚乳酸(nano-hydroxyapatite/collagen/polylactic acid,nHAC/PLA)复合后形成的活性nHAC/PLA(active nHAC/PLA,AnHAC/PLA)修复颅骨极限缺损的效果。方法新西兰大白兔48只,体重2.0～2.5kg。制备直径为15mm的颅骨全层缺损模型,随机分成4组,每组12只。阳性对照组:缺损区植入自体髂骨;空白对照组:缺损区不植入任何材料;阴性对照组:缺损区植入nHAC/PLA;实验组:缺损区植入AnHAC/PLA,每块AnHAC/PLA上平均吸附rhBMP-21.431mg。术后8、16周通过比较颅骨缺损区X线阻射面积占总缺损面积百分比、HE染色和Masson's三色法染色观察颅骨极限缺损的修复情况。结果术后8、16周,各组颅骨缺损区X线阻射程度,阳性对照组分别为67.21%±2.06%、86.48%±1.73%,空白对照组分别为5.84%±1.92%、9.48%±2.72%,阴性对照组分别为19.13%±2.51%、35.67%±3.28%,实验组分别为58.84%±2.55%、85.61%±3.36%。其中除16周实验组与阳性对照组以及空白对照组8、16周比较差异无统计学意义(P>0.05)外,其余各组各时间点比较差异均有统计学意义(P<0.05)。组织学观察显示,阳性对照组骨缺损区16周骨小梁较8周增宽,被大量骨组织充填;空白对照组8、16周骨缺损区均被纤维组织充填,无新骨生成;阴性对照16周骨缺损区为剩余材料与纤维组织充填,周边新骨较8周形成增多;实验组8周材料植入区为新生骨替代,16周新生骨呈板层状,缺损区材料残留较少,且周围可见较多成骨细胞。结论nHAC/PLA是rhBMP-2的良好载体,两者复合后制备的AnHAC/PLA具有良好骨形成能力,有望应用于临床上修复较大型骨缺损。展开更多
文摘Electro-spinning is a very modern process which can be used in various purposes. We did this experimental work at Swerea IVF in Sweden during M. Sc in Textile Technology programme at University of Bor?s. We should especially thank our supervisor—Anna Thorvaldsson and course teacher—Ioannis S. Chronakis. In this report, we have tried to explain the basic manufacturing techniques of the electrospun nanofiber by the electro-spinning, how one can characterize it by SEM (Scanning Electron Microscopy) and its various applications in the practical field, e.g wound healing, Tissue Engineering Scaffold. The experimental work helped us a lot to gather sufficient knowledge about the electro-spinning process which we wanted to share with all.
文摘目的检测重组人骨形成蛋白2(recombinant human bone morphogenetic protein2,rhBMP-2)与纳米羟基磷灰石/胶原/聚乳酸(nano-hydroxyapatite/collagen/polylactic acid,nHAC/PLA)复合后形成的活性nHAC/PLA(active nHAC/PLA,AnHAC/PLA)修复颅骨极限缺损的效果。方法新西兰大白兔48只,体重2.0～2.5kg。制备直径为15mm的颅骨全层缺损模型,随机分成4组,每组12只。阳性对照组:缺损区植入自体髂骨;空白对照组:缺损区不植入任何材料;阴性对照组:缺损区植入nHAC/PLA;实验组:缺损区植入AnHAC/PLA,每块AnHAC/PLA上平均吸附rhBMP-21.431mg。术后8、16周通过比较颅骨缺损区X线阻射面积占总缺损面积百分比、HE染色和Masson's三色法染色观察颅骨极限缺损的修复情况。结果术后8、16周,各组颅骨缺损区X线阻射程度,阳性对照组分别为67.21%±2.06%、86.48%±1.73%,空白对照组分别为5.84%±1.92%、9.48%±2.72%,阴性对照组分别为19.13%±2.51%、35.67%±3.28%,实验组分别为58.84%±2.55%、85.61%±3.36%。其中除16周实验组与阳性对照组以及空白对照组8、16周比较差异无统计学意义(P>0.05)外,其余各组各时间点比较差异均有统计学意义(P<0.05)。组织学观察显示,阳性对照组骨缺损区16周骨小梁较8周增宽,被大量骨组织充填;空白对照组8、16周骨缺损区均被纤维组织充填,无新骨生成;阴性对照16周骨缺损区为剩余材料与纤维组织充填,周边新骨较8周形成增多;实验组8周材料植入区为新生骨替代,16周新生骨呈板层状,缺损区材料残留较少,且周围可见较多成骨细胞。结论nHAC/PLA是rhBMP-2的良好载体,两者复合后制备的AnHAC/PLA具有良好骨形成能力,有望应用于临床上修复较大型骨缺损。
