目的研究加味威草胶囊对尿酸性肾病大鼠肾组织核转录因-κBp50(NF-κBp50)、环氧化酶-2(COX-2)蛋白及mRNA的影响。方法将36只35日龄的SPF级SD随机分为空白组、模型组、加味威草胶囊低剂量组、加味威草胶囊中剂量组、加味威草胶囊高剂量...目的研究加味威草胶囊对尿酸性肾病大鼠肾组织核转录因-κBp50(NF-κBp50)、环氧化酶-2(COX-2)蛋白及mRNA的影响。方法将36只35日龄的SPF级SD随机分为空白组、模型组、加味威草胶囊低剂量组、加味威草胶囊中剂量组、加味威草胶囊高剂量组、依托考昔组,每组6只。除空白组外,其余组均以腺嘌呤+酵母粉混合饲料喂养的方式复制尿酸性肾病大鼠模型。造模成功后,空白组每日给予蒸馏水10 m L/kg灌胃;依托考昔组每日给予依托考昔3.3 mg/kg灌胃,加味威草胶囊低、中、高剂量组分别每日给予加味威草胶囊0.3,0.6,1.2 g/kg灌胃,均连续4周。检测各组大鼠血清尿酸(UA)、尿素(Urea)、肌酐(Cr)水平和肾组织中NF-κBp50、COX-2蛋白及mRNA表达水平。结果模型组及各给药组血清UA、Urea、Cr水平和肾组织中NF-κBp50、COX-2蛋白及mRNA表达水平均明显高于空白组(P均<0.05),加味威草胶囊各剂量组血清UA、Urea、Cr水平和肾组织中NF-κBp50、COX-2蛋白及mRNA表达水平均明显低于模型组(P均<0.05),加味威草胶囊中、高剂量组血清UA、Urea、Cr水平及肾组织中NF-κBp50、COX-2蛋白水平均明显低于依托考昔组(P均<0.05),加味威草胶囊中、高剂量组血清UA、Urea、Cr水平及肾组织中NF-κBp50蛋白表达水平均明显低于加味威草胶囊低剂量组(P均<0.05),加味威草胶囊高剂量组COX-2蛋白和NF-κBp50、COX-2mRNA表达水平均明显低于加味威草胶囊低剂量组(P均<0.05)。结论尿酸性肾病大鼠肾脏中存在NF-κBp50、COX-2的激活,加味威草胶囊对二者有一定的抑制作用,推测抑制NF-κBp50、COX-2的激活是加味威草胶囊抑制肾脏炎症反应、保护肾脏的重要机制之一。展开更多
目的:研究NF-κBp50、JAB1和CRM1在弥漫性大B细胞淋巴瘤(diffuse large B-cell lymphoma,DLBCL)中的表达及其意义。方法:采用免疫组化方法检测94例DLBCL中NF-κBp50、JAB1和CRM1的表达。结果:NF-κBp50、JAB1和CRM1在94例DLBCL的阳性表...目的:研究NF-κBp50、JAB1和CRM1在弥漫性大B细胞淋巴瘤(diffuse large B-cell lymphoma,DLBCL)中的表达及其意义。方法:采用免疫组化方法检测94例DLBCL中NF-κBp50、JAB1和CRM1的表达。结果:NF-κBp50、JAB1和CRM1在94例DLBCL的阳性表达率分别为70.2%(66/94)、77.7%(73/94)和69.1%(65/94)。NF-κBp50在DL-BCL中非生发中心型(non-GCB)中表达明显高于生发中心型(GCB),差异有统计学意义(P<0.01),但JAB1、CRM1与DLBCL分型无关(P>0.05)。NF-κBp50、JAB1和CRM1与DLBCL患者性别、年龄、发病部位均无关(P>0.05)。DL-BCL中NF-κBp50与JAB1、NF-κBp50与CRM1的表达均呈正相关(rs=0.209,P<0.05;rs=0.270,P<0.01)。结论:NF-κp50在DLBCL不同亚型之间表达的差异,可能有助于DLBCL亚型的诊断和预后的判断。JAB1和CRM1对NF-κBp50表达具有正向调节作用。展开更多
Objective:To explore the relationship between expressions of NF-κBp50,p53 and Bcl-2 in tissue of cervical can-cer and human papillomavirus(HPV)infection.Methods:The expressions of NF-κBp50,p53 and Bcl-2 were detecte...Objective:To explore the relationship between expressions of NF-κBp50,p53 and Bcl-2 in tissue of cervical can-cer and human papillomavirus(HPV)infection.Methods:The expressions of NF-κBp50,p53 and Bcl-2 were detected using immuohistochemical staining in 46 specimens of cervical cancer and 26 specimens of normal cervical tissue.The infection of HPV DNA were determined by PCR.Results:The expressions of NF-κBp50,p53 and Bcl-2 in tissue of cervical cancer were significantly higher than that in normal cervical tissue(P<0.01),and the expressions of NF-κBp50 and p53 or Bcl-2 were closely related(P<0.05).The expression of NF-κBp50 in HPV DNA positive group was significantly higher than that in HPV negative group(P<0.05),but there were no significantly differences in the expressions of p53 and Bcl-2 between HPV DNA positive group and HPV negative group(P>0.05).Conclusion:The expressions of NF-κBp50,p53 and Bcl-2 were significantly correlated with cervical carcinogenesis.NF-κBp50 may be activated by HPV infection.展开更多
文摘目的研究加味威草胶囊对尿酸性肾病大鼠肾组织核转录因-κBp50(NF-κBp50)、环氧化酶-2(COX-2)蛋白及mRNA的影响。方法将36只35日龄的SPF级SD随机分为空白组、模型组、加味威草胶囊低剂量组、加味威草胶囊中剂量组、加味威草胶囊高剂量组、依托考昔组,每组6只。除空白组外,其余组均以腺嘌呤+酵母粉混合饲料喂养的方式复制尿酸性肾病大鼠模型。造模成功后,空白组每日给予蒸馏水10 m L/kg灌胃;依托考昔组每日给予依托考昔3.3 mg/kg灌胃,加味威草胶囊低、中、高剂量组分别每日给予加味威草胶囊0.3,0.6,1.2 g/kg灌胃,均连续4周。检测各组大鼠血清尿酸(UA)、尿素(Urea)、肌酐(Cr)水平和肾组织中NF-κBp50、COX-2蛋白及mRNA表达水平。结果模型组及各给药组血清UA、Urea、Cr水平和肾组织中NF-κBp50、COX-2蛋白及mRNA表达水平均明显高于空白组(P均<0.05),加味威草胶囊各剂量组血清UA、Urea、Cr水平和肾组织中NF-κBp50、COX-2蛋白及mRNA表达水平均明显低于模型组(P均<0.05),加味威草胶囊中、高剂量组血清UA、Urea、Cr水平及肾组织中NF-κBp50、COX-2蛋白水平均明显低于依托考昔组(P均<0.05),加味威草胶囊中、高剂量组血清UA、Urea、Cr水平及肾组织中NF-κBp50蛋白表达水平均明显低于加味威草胶囊低剂量组(P均<0.05),加味威草胶囊高剂量组COX-2蛋白和NF-κBp50、COX-2mRNA表达水平均明显低于加味威草胶囊低剂量组(P均<0.05)。结论尿酸性肾病大鼠肾脏中存在NF-κBp50、COX-2的激活,加味威草胶囊对二者有一定的抑制作用,推测抑制NF-κBp50、COX-2的激活是加味威草胶囊抑制肾脏炎症反应、保护肾脏的重要机制之一。
文摘目的:研究NF-κBp50、JAB1和CRM1在弥漫性大B细胞淋巴瘤(diffuse large B-cell lymphoma,DLBCL)中的表达及其意义。方法:采用免疫组化方法检测94例DLBCL中NF-κBp50、JAB1和CRM1的表达。结果:NF-κBp50、JAB1和CRM1在94例DLBCL的阳性表达率分别为70.2%(66/94)、77.7%(73/94)和69.1%(65/94)。NF-κBp50在DL-BCL中非生发中心型(non-GCB)中表达明显高于生发中心型(GCB),差异有统计学意义(P<0.01),但JAB1、CRM1与DLBCL分型无关(P>0.05)。NF-κBp50、JAB1和CRM1与DLBCL患者性别、年龄、发病部位均无关(P>0.05)。DL-BCL中NF-κBp50与JAB1、NF-κBp50与CRM1的表达均呈正相关(rs=0.209,P<0.05;rs=0.270,P<0.01)。结论:NF-κp50在DLBCL不同亚型之间表达的差异,可能有助于DLBCL亚型的诊断和预后的判断。JAB1和CRM1对NF-κBp50表达具有正向调节作用。
基金Supported by a grant from the scientific research foundation of Wenzhou Science and Technology Bureau(No.S2001A30).
文摘Objective:To explore the relationship between expressions of NF-κBp50,p53 and Bcl-2 in tissue of cervical can-cer and human papillomavirus(HPV)infection.Methods:The expressions of NF-κBp50,p53 and Bcl-2 were detected using immuohistochemical staining in 46 specimens of cervical cancer and 26 specimens of normal cervical tissue.The infection of HPV DNA were determined by PCR.Results:The expressions of NF-κBp50,p53 and Bcl-2 in tissue of cervical cancer were significantly higher than that in normal cervical tissue(P<0.01),and the expressions of NF-κBp50 and p53 or Bcl-2 were closely related(P<0.05).The expression of NF-κBp50 in HPV DNA positive group was significantly higher than that in HPV negative group(P<0.05),but there were no significantly differences in the expressions of p53 and Bcl-2 between HPV DNA positive group and HPV negative group(P>0.05).Conclusion:The expressions of NF-κBp50,p53 and Bcl-2 were significantly correlated with cervical carcinogenesis.NF-κBp50 may be activated by HPV infection.
