Acipenseriformes is an endangered primitive fish group, which occupies a special place in the history of ideas concerning fish evolution, even in vertebrate evolution. However, the classification and evolution of the ...Acipenseriformes is an endangered primitive fish group, which occupies a special place in the history of ideas concerning fish evolution, even in vertebrate evolution. However, the classification and evolution of the fishes have been debated. The mitochondrial DMA (mtDNA) ND4L and partial A7D4 genes were first sequenced in twelve species of the order Acipenseriformes, including endemic Chinese species. The following points were drawn from DNA sequences analysis: (i) the two species of Huso can be ascribed to Acipenser; (ii) A. dabryanus is the mostly closely related to A. sinensis, and most likely the landlocked form of A. sinensis; (iii) genus Acipenser in trans-Pacific region might have a common origin; (iv) mtDNA ND4L and ND4 genes are the ideal genetic markers for phylogenetic analysis of the order Acipenseriformes.展开更多
The relationships within Drosophila melanogaster species group are controversial from morphology, chromosomes and DNA sequences. This study utilises a molecular approach aimed at uncovering the phylogenetic relationsh...The relationships within Drosophila melanogaster species group are controversial from morphology, chromosomes and DNA sequences. This study utilises a molecular approach aimed at uncovering the phylogenetic relationships among 33 taxa representing 8 subgroups of D. melanogaster species groups. Mitocondrial ND4L-ND4 was sequenced in the all 8 subgroups covering a wide geographic area. MP and Bayesian analysis produced an identical tree topology with relatively strong support in most nodes. It reveals that the melanogaster species group clustered in three main lineages:1)montium subgroup; 2) ananassae subgroup; 3) Oriental subgroups ( melanogaster, ficsphila, eugracilis, elegans, syzukii and takahashii). The montium subgroup branched off first, followed by the ananassae subgroup. In the third lineage, melanogaster is the most divergent subgroup followed by ficsphila, eugracilis, elegans in that order. The suzukii and takahashii sister subgroups are the last to branch off.展开更多
本实验对黑腹果蝇种组(melanogaster species group)中8个种亚组33个样品两个线粒体基因ND4和ND4L进行了测序,并分析了ND4基因的序列差异和碱基替换特点,发现近缘物种中存在很明显的转换倾向,而在远缘物种中由于重复替换导致转换数处于...本实验对黑腹果蝇种组(melanogaster species group)中8个种亚组33个样品两个线粒体基因ND4和ND4L进行了测序,并分析了ND4基因的序列差异和碱基替换特点,发现近缘物种中存在很明显的转换倾向,而在远缘物种中由于重复替换导致转换数处于饱和状态,我们的实验数据证实了线粒体基因较核基因有较快的进化速度。最后根据D.melanogaster与D.yakuba的遗传距离推算了8个种亚组的分化时间,ananassae种亚组最先分化,然后依次是montium,melanogaster,fic-sphila,eugracilis,elegans,suzukii和takahashii最后分化。展开更多
文摘Acipenseriformes is an endangered primitive fish group, which occupies a special place in the history of ideas concerning fish evolution, even in vertebrate evolution. However, the classification and evolution of the fishes have been debated. The mitochondrial DMA (mtDNA) ND4L and partial A7D4 genes were first sequenced in twelve species of the order Acipenseriformes, including endemic Chinese species. The following points were drawn from DNA sequences analysis: (i) the two species of Huso can be ascribed to Acipenser; (ii) A. dabryanus is the mostly closely related to A. sinensis, and most likely the landlocked form of A. sinensis; (iii) genus Acipenser in trans-Pacific region might have a common origin; (iv) mtDNA ND4L and ND4 genes are the ideal genetic markers for phylogenetic analysis of the order Acipenseriformes.
文摘The relationships within Drosophila melanogaster species group are controversial from morphology, chromosomes and DNA sequences. This study utilises a molecular approach aimed at uncovering the phylogenetic relationships among 33 taxa representing 8 subgroups of D. melanogaster species groups. Mitocondrial ND4L-ND4 was sequenced in the all 8 subgroups covering a wide geographic area. MP and Bayesian analysis produced an identical tree topology with relatively strong support in most nodes. It reveals that the melanogaster species group clustered in three main lineages:1)montium subgroup; 2) ananassae subgroup; 3) Oriental subgroups ( melanogaster, ficsphila, eugracilis, elegans, syzukii and takahashii). The montium subgroup branched off first, followed by the ananassae subgroup. In the third lineage, melanogaster is the most divergent subgroup followed by ficsphila, eugracilis, elegans in that order. The suzukii and takahashii sister subgroups are the last to branch off.
文摘本实验对黑腹果蝇种组(melanogaster species group)中8个种亚组33个样品两个线粒体基因ND4和ND4L进行了测序,并分析了ND4基因的序列差异和碱基替换特点,发现近缘物种中存在很明显的转换倾向,而在远缘物种中由于重复替换导致转换数处于饱和状态,我们的实验数据证实了线粒体基因较核基因有较快的进化速度。最后根据D.melanogaster与D.yakuba的遗传距离推算了8个种亚组的分化时间,ananassae种亚组最先分化,然后依次是montium,melanogaster,fic-sphila,eugracilis,elegans,suzukii和takahashii最后分化。