目的通过膀胱灌注N蛳甲基亚硝基脲(M N U)诱导近交系大鼠膀胱肿瘤。方法选取F344大鼠20只,膀胱灌注M N U,每2周1次,共5次;于第14周处死动物,取膀胱肿瘤组织测M H C蛳I及病理观察。结果M N U组肿瘤发生率为100%;膀胱肿瘤的M H C蛳I表达较...目的通过膀胱灌注N蛳甲基亚硝基脲(M N U)诱导近交系大鼠膀胱肿瘤。方法选取F344大鼠20只,膀胱灌注M N U,每2周1次,共5次;于第14周处死动物,取膀胱肿瘤组织测M H C蛳I及病理观察。结果M N U组肿瘤发生率为100%;膀胱肿瘤的M H C蛳I表达较高(58.60%￣93.80%)。结论M N U可成功诱导F344大鼠膀胱肿瘤的形成,是肿瘤免疫治疗的理想模型。展开更多
Photoreceptor cell degeneration leads to blindness, for which there is currently no effective treatment. Our previous studies have shown that Lycium barbarum(L. barbarum) polysaccharide(LBP) protects degenerated photo...Photoreceptor cell degeneration leads to blindness, for which there is currently no effective treatment. Our previous studies have shown that Lycium barbarum(L. barbarum) polysaccharide(LBP) protects degenerated photoreceptors in rd1, a transgenic mouse model of retinitis pigmentosa. L. barbarum glycopeptide(Lb GP) is an immunoreactive glycoprotein extracted from LBP. In this study, we investigated the potential protective effect of Lb GP on a chemically induced photoreceptor-degenerative mouse model. Wild-type mice received the following: oral administration of Lb GP as a protective pre-treatment on days 1–7;intraperitoneal administration of 40 mg/kg N-methylN-nitrosourea to induce photoreceptor injury on day 7;and continuation of orally administered Lb GP on days 8–14. Treatment with Lb GP increased photoreceptor survival and improved the structure of photoreceptors, retinal photoresponse, and visual behaviors of mice with photoreceptor degeneration. Lb GP was also found to partially inhibit the activation of microglia in N-methyl-N-nitrosourea-injured retinas and significantly decreased the expression of two pro-inflammatory cytokines. In conclusion, Lb GP effectively slowed the rate of photoreceptor degeneration in N-methyl-N-nitrosourea-injured mice, possibly through an anti-inflammatory mechanism, and has potential as a candidate drug for the clinical treatment of photoreceptor degeneration.展开更多
文摘目的通过膀胱灌注N蛳甲基亚硝基脲(M N U)诱导近交系大鼠膀胱肿瘤。方法选取F344大鼠20只,膀胱灌注M N U,每2周1次,共5次;于第14周处死动物,取膀胱肿瘤组织测M H C蛳I及病理观察。结果M N U组肿瘤发生率为100%;膀胱肿瘤的M H C蛳I表达较高(58.60%￣93.80%)。结论M N U可成功诱导F344大鼠膀胱肿瘤的形成,是肿瘤免疫治疗的理想模型。
基金supported by Guangzhou Key Projects of Brain Science and Brain-Like Intelligence Technology,No.20200730009 (to YX)the National Natural Science Foundation of China,No.82074169 (to XM)+2 种基金the Guangdong Basic and Applied Basic Research Foundation,No.2021A1515012473 (to XM)Project of Administration of Traditional Chinese Medicine of Guangdong Province,No.20202045 (to XM)Aier Eye Hospital Group,No.AF2019001 (to ST,KFS,YX,XM)。
文摘Photoreceptor cell degeneration leads to blindness, for which there is currently no effective treatment. Our previous studies have shown that Lycium barbarum(L. barbarum) polysaccharide(LBP) protects degenerated photoreceptors in rd1, a transgenic mouse model of retinitis pigmentosa. L. barbarum glycopeptide(Lb GP) is an immunoreactive glycoprotein extracted from LBP. In this study, we investigated the potential protective effect of Lb GP on a chemically induced photoreceptor-degenerative mouse model. Wild-type mice received the following: oral administration of Lb GP as a protective pre-treatment on days 1–7;intraperitoneal administration of 40 mg/kg N-methylN-nitrosourea to induce photoreceptor injury on day 7;and continuation of orally administered Lb GP on days 8–14. Treatment with Lb GP increased photoreceptor survival and improved the structure of photoreceptors, retinal photoresponse, and visual behaviors of mice with photoreceptor degeneration. Lb GP was also found to partially inhibit the activation of microglia in N-methyl-N-nitrosourea-injured retinas and significantly decreased the expression of two pro-inflammatory cytokines. In conclusion, Lb GP effectively slowed the rate of photoreceptor degeneration in N-methyl-N-nitrosourea-injured mice, possibly through an anti-inflammatory mechanism, and has potential as a candidate drug for the clinical treatment of photoreceptor degeneration.
