Mycobacterium tuberculosis （ M. tuberculosis） is a pathogen associated with the deaths of millions of people worldwide annually. Although tuberculosis is a treatable disease, the emergence and increasing prevalence...Mycobacterium tuberculosis （ M. tuberculosis） is a pathogen associated with the deaths of millions of people worldwide annually. Although tuberculosis is a treatable disease, the emergence and increasing prevalence of multi-drug resistant bacterial pathogens emphasizes the need for new and innovative anti-microbial strategies. Effective therapeutic regimens, which have existed, are limited by the emergence of drug resistance and the inability of antibiotics to kill dormant or intracellular organisms.展开更多
目的采用Meta分析的方法评价噬菌体生物扩增法检测临床标本中结核分枝杆菌的准确性。方法通过检索PubMed、EMbase、web of science、Cochrane等数据库和其他方式广泛收集文献。根据QUADAS质量评价标准评价纳入文献的质量,应用Meta-Disc...目的采用Meta分析的方法评价噬菌体生物扩增法检测临床标本中结核分枝杆菌的准确性。方法通过检索PubMed、EMbase、web of science、Cochrane等数据库和其他方式广泛收集文献。根据QUADAS质量评价标准评价纳入文献的质量,应用Meta-Disc软件对噬菌体生物扩增法检测临床标本中结核分枝杆菌的敏感性、特异性、诊断比数比进行合并分析,并进行异质性检验,对纳入研究绘制SROC曲线。结果最终纳入19篇相关文献,共计20个研究。综合分析,合并特异性为96%(96-97%);合并敏感度为69%(67-72%);合并诊断比数比为52.30(25.18-108.65);SROC曲线下面积为0.93。结论对纳入的研究分析证实,噬菌体检测法检测临床标本中的结核分枝杆菌的敏感性偏低,但具有高度特异性,表明该方法在快速诊断结核病的准确性方面具有优势。展开更多
Environmental mycobacteria are capable of forming biofilms in low-nutrient environments, and these biofilms may act as reservoirs for opportunistic infections. The purpose of this study was to determine if bacteriopha...Environmental mycobacteria are capable of forming biofilms in low-nutrient environments, and these biofilms may act as reservoirs for opportunistic infections. The purpose of this study was to determine if bacteriophages could disrupt existing biofilms of acid-fast staining Mycobacterium smegmatis. Using the MBEC 96-well plastic peg assay system, M. smegmatis biofilms were created and then tested for their stability in the presence of mycobacteriophages isolated from a Minnesota sphagnum peat bog. All phages tested were lytic and were observed to have weak, intermediate, and strong abilities to disrupt M. smegmatis biofilms. The formation of biofilms was severely impaired in the presence of mycobacteriophages. Phage treatment was also shown to augment?M. smegmatis biofilm disruption by mechanical forces of sonication or water flow. Our study shows that, as with biofilms of Gram-positive and Gram-negative bacteria, mycobacterial biofilms are also susceptible to destruction by bacteriophages.展开更多
Background: The article outlines the ways of preparing and sowing various samples for research with a view to isolating mycobacteriophages (MBphages), as well as developing methods for their isolation. Methods: Experi...Background: The article outlines the ways of preparing and sowing various samples for research with a view to isolating mycobacteriophages (MBphages), as well as developing methods for their isolation. Methods: Experimental studies on the obtaining of MB phages active against tubercle mycobacteria were conducted using samples collected from environmental objects and biological material from the different areas of the country. As an indicator of test-cultures, we have used the following M. kansasii, M. avium, M. scrofulaseum, M. phlei, M. terrae, М. intracellulare, M. smegmatis. For the cultivation of Mycobacteria and MB phages were used the following nutrient media of the company HiMedia: Dubos Broth Base, Dubos Oleic Agar Base, Kirschner Medium Base-Modified, Middlebrook 7H10 Agar Base, Peizer TB Medium Base, Lowenstein Jensen medium base и Sauton’s Fluid Medium. Results: During the research, the following results were obtained: An effective method for obtaining MB phages from environmental objects was select-ed. Four types of Mbphage were obtained, lysing atypical mycobacteria from environmental objects delivered from various areas of the Republic of Ka-zakhstan. Conclusion: Obtained data in the development of the method of obtaining MBphage, allows us to consider it necessary to further study their biological features, as well as the possibility of using them to develop an an-ti-tuberculosis effective agent.展开更多
文摘Mycobacterium tuberculosis （ M. tuberculosis） is a pathogen associated with the deaths of millions of people worldwide annually. Although tuberculosis is a treatable disease, the emergence and increasing prevalence of multi-drug resistant bacterial pathogens emphasizes the need for new and innovative anti-microbial strategies. Effective therapeutic regimens, which have existed, are limited by the emergence of drug resistance and the inability of antibiotics to kill dormant or intracellular organisms.
文摘目的采用Meta分析的方法评价噬菌体生物扩增法检测临床标本中结核分枝杆菌的准确性。方法通过检索PubMed、EMbase、web of science、Cochrane等数据库和其他方式广泛收集文献。根据QUADAS质量评价标准评价纳入文献的质量,应用Meta-Disc软件对噬菌体生物扩增法检测临床标本中结核分枝杆菌的敏感性、特异性、诊断比数比进行合并分析,并进行异质性检验,对纳入研究绘制SROC曲线。结果最终纳入19篇相关文献,共计20个研究。综合分析,合并特异性为96%(96-97%);合并敏感度为69%(67-72%);合并诊断比数比为52.30(25.18-108.65);SROC曲线下面积为0.93。结论对纳入的研究分析证实,噬菌体检测法检测临床标本中的结核分枝杆菌的敏感性偏低,但具有高度特异性,表明该方法在快速诊断结核病的准确性方面具有优势。
文摘Environmental mycobacteria are capable of forming biofilms in low-nutrient environments, and these biofilms may act as reservoirs for opportunistic infections. The purpose of this study was to determine if bacteriophages could disrupt existing biofilms of acid-fast staining Mycobacterium smegmatis. Using the MBEC 96-well plastic peg assay system, M. smegmatis biofilms were created and then tested for their stability in the presence of mycobacteriophages isolated from a Minnesota sphagnum peat bog. All phages tested were lytic and were observed to have weak, intermediate, and strong abilities to disrupt M. smegmatis biofilms. The formation of biofilms was severely impaired in the presence of mycobacteriophages. Phage treatment was also shown to augment?M. smegmatis biofilm disruption by mechanical forces of sonication or water flow. Our study shows that, as with biofilms of Gram-positive and Gram-negative bacteria, mycobacterial biofilms are also susceptible to destruction by bacteriophages.
文摘Background: The article outlines the ways of preparing and sowing various samples for research with a view to isolating mycobacteriophages (MBphages), as well as developing methods for their isolation. Methods: Experimental studies on the obtaining of MB phages active against tubercle mycobacteria were conducted using samples collected from environmental objects and biological material from the different areas of the country. As an indicator of test-cultures, we have used the following M. kansasii, M. avium, M. scrofulaseum, M. phlei, M. terrae, М. intracellulare, M. smegmatis. For the cultivation of Mycobacteria and MB phages were used the following nutrient media of the company HiMedia: Dubos Broth Base, Dubos Oleic Agar Base, Kirschner Medium Base-Modified, Middlebrook 7H10 Agar Base, Peizer TB Medium Base, Lowenstein Jensen medium base и Sauton’s Fluid Medium. Results: During the research, the following results were obtained: An effective method for obtaining MB phages from environmental objects was select-ed. Four types of Mbphage were obtained, lysing atypical mycobacteria from environmental objects delivered from various areas of the Republic of Ka-zakhstan. Conclusion: Obtained data in the development of the method of obtaining MBphage, allows us to consider it necessary to further study their biological features, as well as the possibility of using them to develop an an-ti-tuberculosis effective agent.
