目的研究新佐剂-Montanide ISA 50V2在马免疫中的应用,制定合理的免疫程序,提高马免疫血清效价。方法用Montanide ISA 50V2乳化A型和F型肉毒类毒素制备成油乳抗原,免疫家兔和马,并与福氏不完全佐剂进行对比。结果在马免疫程序中,Montani...目的研究新佐剂-Montanide ISA 50V2在马免疫中的应用,制定合理的免疫程序,提高马免疫血清效价。方法用Montanide ISA 50V2乳化A型和F型肉毒类毒素制备成油乳抗原,免疫家兔和马,并与福氏不完全佐剂进行对比。结果在马免疫程序中,Montanide ISA 50V2与福氏不完全佐剂交替使用效果更好,免疫效价较高。结论在进一步试验确证后,将Montanide ISA 50V2作为佐剂应用于马免疫中,以获得更高的免疫血清的效价。展开更多
以钥孔戚血蓝蛋白(Keyhole limpet hemocyanin,KLH)为抗原、Montanide ISA 763 A为佐剂开展金鱼免疫试验,检测血清抗体水平,评估金鱼体液免疫应答情况,以期为其免疫策略的制定提供依据。试验在水温15~18℃时进行,采用(20±3)g体重...以钥孔戚血蓝蛋白(Keyhole limpet hemocyanin,KLH)为抗原、Montanide ISA 763 A为佐剂开展金鱼免疫试验,检测血清抗体水平,评估金鱼体液免疫应答情况,以期为其免疫策略的制定提供依据。试验在水温15~18℃时进行,采用(20±3)g体重的健康鱼150尾,随机均分为三组,第一组使用(KLH+Montanide ISA 763 A)免疫,第二组仅使用KLH免疫,第三组注射等量的0.01 mol/L磷酸盐缓冲液(Phosphate buffered solution,PBS)为对照,历经三次免疫并对4次采样的血清样本进行ELISA检测。结果显示,第一组和第二组均检测到较高的抗KLH抗体水平,在第二次、第三次免疫后,添加ISA佐剂的第一组抗体仍维持较高水平,而在仅用KLH免疫的第二组抗体水平下降,并降低到对照组水平,表明使用(KLH+Montanide ISA 763 A)免疫金鱼可以获得更加持久和稳定的免疫效果,而仅注射KLH的免疫效果较为短暂。研究还说明金鱼在低温情况下,可以产生良好的体液免疫应答。展开更多
Hydro-Pericardium Syndrome (HPS) is viral problem of commercial poultry caused by aviadeno virus type-4. In Pakistan the problems have been controlled by administering inactivated infected liver homogenate vaccine (IL...Hydro-Pericardium Syndrome (HPS) is viral problem of commercial poultry caused by aviadeno virus type-4. In Pakistan the problems have been controlled by administering inactivated infected liver homogenate vaccine (ILHV). The use of liver based HPS vaccines remained potential threat for having hypersensitivity reactions in poultry. The current study was carried out to compare the serological potency of HPS ILHV to vero cell line adopted vaccine in term of anti HPS-ELISA antibody titers. 14 HPS virus vaccines were prepared based on different concentration of antigen, type of adjuvants and source of virus substrate. Total of 160 birds were divided into 16 groups each containing 10 birds. At day of 14th age each bird of every group was injected with 0.3 ml dose of respective vaccine. It was observed that HPS infected liver based vaccine having 1 × 105.6, 1 × 105.6 and 1 × 103.6 bird lethal dose 50 induced 1092.10, 875.25 and 702.2 anti-HPS ELISA antibody titer respectively. The 20, 25 and 30 doses/gm HPS infected liver vaccine induced 110.4, 1071.9 and 1037.8 anti-HPS ELISA antibody titer respectively. Montanide based tissue culture HPS vaccine showed significantly higher 1148.45 anti-HPS ELISA antibody titer to aluminium hydroxide based vaccine (137.2) (P 5.6 TCID50 is serological potent against field infection. The vaccines based on such formulation could be prepared in future for effective immuno-prophylaxis against HPS virus.展开更多
Objective This study aimed to investigate the effects of Montanide ISA-720 and Naloxone(NLX)in Hepatitis B surface antigen(HBsAg)vaccine formulation on cytokine and long-lasting antibody responses.Methods First,the HB...Objective This study aimed to investigate the effects of Montanide ISA-720 and Naloxone(NLX)in Hepatitis B surface antigen(HBsAg)vaccine formulation on cytokine and long-lasting antibody responses.Methods First,the HBsAg was formulated in Montanide ISA-720 adjuvant and Naloxone at 5 and 10mg/kg.The experimental mice were immunized three times at a 2-week interval,and then IL-4,IL-2,TNF-α,and IFN-γcytokines;long-lasting IgG antibody responses 220 days after the last shot;and IgG1/IgG2a isotypes were assessed by ELISA.Results The HBsAg-Alum group exhibited the highest IL-4 cytokine response among the experimental groups,whereas NLX in HBsAg-MON720 vaccine formulation did not affect cytokine responses.In addition,NLX in Alum-based vaccine suppressed IL-4 cytokine response and increased the IL-2/IL-4 cytokine ratio.Moreover,HBsAg-MON720 was more potent than HBsAg-Alum in the induction of antibody responses,and NLX in Alum-and MON720-based vaccines induced long-lasting antibody responses.Conclusion NLX in Alum-based vaccine decreased IL-4 cytokine response,increased IL-2/IL-4 cytokine ratio,and improved long-lasting humoral immune responses in both vaccine formulations.Therefore,the adjuvant activity of NLX in the vaccine formulation depends on the type of adjuvant and the nature of the antigen in the vaccine formulation.展开更多
文摘目的研究新佐剂-Montanide ISA 50V2在马免疫中的应用,制定合理的免疫程序,提高马免疫血清效价。方法用Montanide ISA 50V2乳化A型和F型肉毒类毒素制备成油乳抗原,免疫家兔和马,并与福氏不完全佐剂进行对比。结果在马免疫程序中,Montanide ISA 50V2与福氏不完全佐剂交替使用效果更好,免疫效价较高。结论在进一步试验确证后,将Montanide ISA 50V2作为佐剂应用于马免疫中,以获得更高的免疫血清的效价。
文摘以钥孔戚血蓝蛋白(Keyhole limpet hemocyanin,KLH)为抗原、Montanide ISA 763 A为佐剂开展金鱼免疫试验,检测血清抗体水平,评估金鱼体液免疫应答情况,以期为其免疫策略的制定提供依据。试验在水温15~18℃时进行,采用(20±3)g体重的健康鱼150尾,随机均分为三组,第一组使用(KLH+Montanide ISA 763 A)免疫,第二组仅使用KLH免疫,第三组注射等量的0.01 mol/L磷酸盐缓冲液(Phosphate buffered solution,PBS)为对照,历经三次免疫并对4次采样的血清样本进行ELISA检测。结果显示,第一组和第二组均检测到较高的抗KLH抗体水平,在第二次、第三次免疫后,添加ISA佐剂的第一组抗体仍维持较高水平,而在仅用KLH免疫的第二组抗体水平下降,并降低到对照组水平,表明使用(KLH+Montanide ISA 763 A)免疫金鱼可以获得更加持久和稳定的免疫效果,而仅注射KLH的免疫效果较为短暂。研究还说明金鱼在低温情况下,可以产生良好的体液免疫应答。
文摘Hydro-Pericardium Syndrome (HPS) is viral problem of commercial poultry caused by aviadeno virus type-4. In Pakistan the problems have been controlled by administering inactivated infected liver homogenate vaccine (ILHV). The use of liver based HPS vaccines remained potential threat for having hypersensitivity reactions in poultry. The current study was carried out to compare the serological potency of HPS ILHV to vero cell line adopted vaccine in term of anti HPS-ELISA antibody titers. 14 HPS virus vaccines were prepared based on different concentration of antigen, type of adjuvants and source of virus substrate. Total of 160 birds were divided into 16 groups each containing 10 birds. At day of 14th age each bird of every group was injected with 0.3 ml dose of respective vaccine. It was observed that HPS infected liver based vaccine having 1 × 105.6, 1 × 105.6 and 1 × 103.6 bird lethal dose 50 induced 1092.10, 875.25 and 702.2 anti-HPS ELISA antibody titer respectively. The 20, 25 and 30 doses/gm HPS infected liver vaccine induced 110.4, 1071.9 and 1037.8 anti-HPS ELISA antibody titer respectively. Montanide based tissue culture HPS vaccine showed significantly higher 1148.45 anti-HPS ELISA antibody titer to aluminium hydroxide based vaccine (137.2) (P 5.6 TCID50 is serological potent against field infection. The vaccines based on such formulation could be prepared in future for effective immuno-prophylaxis against HPS virus.
基金supported by a grant from the Deputy of Research and Technology,Health Ministry of Iran[Grant no.642]partially by a grant from the Pasteur Institute of Iran。
文摘Objective This study aimed to investigate the effects of Montanide ISA-720 and Naloxone(NLX)in Hepatitis B surface antigen(HBsAg)vaccine formulation on cytokine and long-lasting antibody responses.Methods First,the HBsAg was formulated in Montanide ISA-720 adjuvant and Naloxone at 5 and 10mg/kg.The experimental mice were immunized three times at a 2-week interval,and then IL-4,IL-2,TNF-α,and IFN-γcytokines;long-lasting IgG antibody responses 220 days after the last shot;and IgG1/IgG2a isotypes were assessed by ELISA.Results The HBsAg-Alum group exhibited the highest IL-4 cytokine response among the experimental groups,whereas NLX in HBsAg-MON720 vaccine formulation did not affect cytokine responses.In addition,NLX in Alum-based vaccine suppressed IL-4 cytokine response and increased the IL-2/IL-4 cytokine ratio.Moreover,HBsAg-MON720 was more potent than HBsAg-Alum in the induction of antibody responses,and NLX in Alum-and MON720-based vaccines induced long-lasting antibody responses.Conclusion NLX in Alum-based vaccine decreased IL-4 cytokine response,increased IL-2/IL-4 cytokine ratio,and improved long-lasting humoral immune responses in both vaccine formulations.Therefore,the adjuvant activity of NLX in the vaccine formulation depends on the type of adjuvant and the nature of the antigen in the vaccine formulation.
