In this paper we obtain some lower bounds for minus and signed domination numbers. We also prove and generalize a conjecture on the minus domination number for bipartite graph of order n, which was proposed by Jean Du...In this paper we obtain some lower bounds for minus and signed domination numbers. We also prove and generalize a conjecture on the minus domination number for bipartite graph of order n, which was proposed by Jean Dunbar et al [1].展开更多
基于经过非均一性检验及订正前后1979-2003年中国东部地区130个气象台站的日平均、最高和最低温度常规观测资料,利用研究区域内气象台站常规观测资料和NCEP/NCAR(The NationalCenters for Atmospheric Prediction and the National Cent...基于经过非均一性检验及订正前后1979-2003年中国东部地区130个气象台站的日平均、最高和最低温度常规观测资料,利用研究区域内气象台站常规观测资料和NCEP/NCAR(The NationalCenters for Atmospheric Prediction and the National Center for Atmosphetic Research)再分析资料温度变化趋势的差值,本文定量考察了研究区域内城市化及土地利用类型改变对区域温度气候趋势的贡献.研究结果显示,经过订正后的台站观测数据具有一定的可信度,比订正前更具合理性.城市化及土地利用类型的改变造成了研究区域内城镇气象站点日平均、最高和最低温度的上升,其中日最低温度的上升程度大于日最高温度,从而导致了日较差温度的下降.进一步研究表明,从1980年代后期开始,城镇气象站点的日平均和最低温度明显上升.从不同季节来看,冬季是区域温度变化趋势最为明显的季节,但却是城市化及土地利用类型改变对区域温度气候趋势贡献最小的季节,对研究区域温度气候趋势贡献最大的季节为夏季.展开更多
The study of minus paired domination of a graph G=(V,E) is initiated. Let SV be any paired dominating set of G , a minus paired dominating function is a function of the form f∶V→{-1,0,1} such that ...The study of minus paired domination of a graph G=(V,E) is initiated. Let SV be any paired dominating set of G , a minus paired dominating function is a function of the form f∶V→{-1,0,1} such that f(v)= 1 for v∈S, f(v)≤0 for v∈V-S , and f(N)≥1 for all v∈V . The weight of a minus paired dominating function f is w(f)=∑f(v) , over all vertices v∈V . The minus paired domination number of a graph G is γ - p( G )=min{ w(f)|f is a minus paired dominating function of G }. On the basis of the minus paired domination number of a graph G defined, some of its properties are discussed.展开更多
For an arbitrary subset P of the reals, a function f ： V →P is defined to be a P-dominating function of a graph G = （V, E） if the sum of its function values over any closed neighbourhood is at least 1. That is, fo...For an arbitrary subset P of the reals, a function f ： V →P is defined to be a P-dominating function of a graph G = （V, E） if the sum of its function values over any closed neighbourhood is at least 1. That is, for every v ∈ V, f（N[v]） ≥ 1. The definition of total P-dominating function is obtained by simply changing ‘closed＇ neighborhood N[v] in the definition of P-dominating function to ‘open＇ neighborhood N（v）. The （total） P-domination number of a graph G is defined to be the infimum of weight w（f） = ∑v ∈ V f（v） taken over all （total） P-dominating function f. Similarly, the P-edge and P-star dominating functions can be defined. In this paper we survey some recent progress on the topic of dominating functions in graph theory. Especially, we are interested in P-, P-edge and P-star dominating functions of graphs with integer values.展开更多
Although the dynamic instability of microtubules(MTs)is fundamental to many cellular functions,quiescent MTs with unattached free distal ends are commonly present and play important roles in various events to power ce...Although the dynamic instability of microtubules(MTs)is fundamental to many cellular functions,quiescent MTs with unattached free distal ends are commonly present and play important roles in various events to power cellular dynamics.However,how these free MT tips are stabilized remains poorly understood.Here,we report that centrosome and spindle pole protein 1(CSPP1)caps and stabilizes both plus and minus ends of static MTs.Real-time imaging of laser-ablated MTs in live cells showed deposition of CSPP1 at the newly generated MT ends,whose dynamic instability was concomitantly suppressed.Consistently,MT ends in CSPP1-overexpressing cells were hyper-stabilized,while those in CSPP1-depleted cells were much more dynamic.This CSPP1-elicited stabilization of MTs was demonstrated to be achieved by suppressing intrinsic MT catastrophe and restricting polymerization.Importantly,CSPP1-bound MTs were resistant to mitotic centromere-associated kinesin-mediated depolymerization.These findings delineate a previously uncharacterized CSPP1 activity that integrates MT end capping to orchestrate quiescent MTs.展开更多
The surface wind speed(SWS)is affected by both large-scale circulation and land use and cover change(LUCC).In China,most studies have considered the effect of large-scale circulation rather than LUCC on SWS.In this st...The surface wind speed(SWS)is affected by both large-scale circulation and land use and cover change(LUCC).In China,most studies have considered the effect of large-scale circulation rather than LUCC on SWS.In this study,we evaluated the effects of LUCC on the SWS decrease during 1979-2015 over China using the observation minus reanalysis(OMR)method.There were two key findings:(1)Observed wind speed declined significantly at a rate of 0.0112 m/(s·a),whereas ERA-Interim,which can only capture the inter-annual variation of observed data,indicated a gentle downward trend.The effects of LUCC on SWS were distinct and caused a decrease of 0.0124 m/(s·a)in SWS;(2)Due to variations in the characteristics of land use types across different regions,the influence of LUCC on SWS also varied.The observed wind speed showed a rapid decline over cultivated land in Northwest China,as well as a decrease in China’s northeastern and eastern plain regions due to the urbanization.However,in the Tibetan Plateau,the impact of LUCC on wind speed was only slight and can thus be ignored.展开更多
The CTD (conductivity, temperature and depth) data collected by six China-Korea joint cruises during 1996-1998 and the climatological data suggest that the seasonal variability of average salinity in the Yellow Sea (S...The CTD (conductivity, temperature and depth) data collected by six China-Korea joint cruises during 1996-1998 and the climatological data suggest that the seasonal variability of average salinity in the Yellow Sea (Sa) presents a general sinusoid pattern. To study the mechanism of the variability, annual cycles of Sa were simulated and a theoretical analysis based on the governing equations was reported.Three main factors are responsible for the variability: the Yellow Sea Warm Current (YSWC), the Changji-ang (Yangtze) River diluted water (YRDW) and the evaporation minus precipitation (E-P). From December to the next May, the variability of Sa is mainly controlled by the salt transportation of the YSWC. But in early July, the YSWC is overtaken and replaced by the YRDW which then becomes the most important controller in summer. From late September to November, the E-P gradually took the lead. The mass exchange north of the 37癗 line is not significant.展开更多
AIM： We designed two synthetic-core-specific peptides core 1 （C1） and core 2 （C2）, and an E1-specific peptide （El）. We produced specific polyclonal antibodies against these peptides and used the antibodies for ...AIM： We designed two synthetic-core-specific peptides core 1 （C1） and core 2 （C2）, and an E1-specific peptide （El）. We produced specific polyclonal antibodies against these peptides and used the antibodies for detection of HCV antigens on surface and within infected peripheral blood leukocytes. METHODS： Peripheral blood from a healthy individual who tested negative for HCV RNA was incubated with HCV type 4 infected serum for i h and 24 h at 37 ℃. Cells were stained by direct and indirect immunofluorescence and measured by flow cytometry. RESULTS： After 1 h of incubation, antibodies against C1, C2, and El detected HCV antigens on the surface of 27%, 26% and 73% of monocytes respectively, while 10%, 5% and 9% of lymphocytes were positive with anti-C1, anti-C2 and anti-E1 respectively. Only 1-3% of granulocytes showed positive staining with anti-C1, anti-C2 and anti E1 antibodies. After 24 h of incubation, we found no surface staining with anti-C1, anti-C2 or anti-E1. Direct immunostaining using anti-C2 could not detect intracellular HCV antigens, after 1 h of incubation with the virus, while after 24 h of incubation, 28% of infected cells showed positive staining. Only plus strand RNA was detectable intracellularly as early as 1 h after incubation, and remained detectable throughout 48 h post-infection. Interestingly, minus RNA strand could not be detected after 1 h, but became strongly detectable intracellularly after 24 h post-infection. CONCLUSION： Monocytes and lymphocytes are the preferred target cells for HCV infection in peripheral blood leukocytes. Our specific anti-core and anti-E1 antibodies are valuable reagents for demonstration of HCV cell cycle. Also, HCV is capable of infecting and replicating in peripheral blood mononuclear cells as confirmed by detection of minus strand HCV RNA as well as intracellular staining of core HCV antigen.展开更多
An upper bound is established on the parameter Γ -(G) for a cubic graph G and two infinite families of 3-connected graphs G k, G * k are constructed to show that the bound is sharp and, moreover, the difference Γ -(...An upper bound is established on the parameter Γ -(G) for a cubic graph G and two infinite families of 3-connected graphs G k, G * k are constructed to show that the bound is sharp and, moreover, the difference Γ -(G * k)-γ s(G * k) can be arbitrarily large, where Г -(G * k) and γ s(G * k) are the upper minus domination and signed domination numbers of G * k, respectively. Thus two open problems are solved.展开更多
Attached cultivation is a promising method for microalgal biomass production. Filamentous oleaginous microalga Tribonema minus(hereafter T. minus) has shown a remarkable potential for biofuel production in terms of it...Attached cultivation is a promising method for microalgal biomass production. Filamentous oleaginous microalga Tribonema minus(hereafter T. minus) has shown a remarkable potential for biofuel production in terms of its high lipid content. However, the strain has only been cultivated in suspended cultivation systems including open pond and closed photobioreactors. Here, we attempted to study the attached cultivation of T. minus, which might be helpful for its scale-up cultivation and industrial applications. As the results, the optimal conditions for T. minus growth in the attached biofilm are 200 μmol photons m^-2 s^-1 of light intensity and 5% of CO2, and the maximum biomass density of 223 g m^-2 has been achieved under the light intensity. The non-woven fabric as substratum was found as the best substratum in thin layer attached bioreactor, on which the average biomass productivity of T. minus is about(9.73 ± 2.19) g m^-2)d^-1. Furthermore, two attached bioreactor systems, rotary drum and rotation disc, were designed following the light dilution strategy and introduced into T. minus cultivation. The highest footprint areal biomass productivity of these two systems is 33 and 47.1 g m^-2 d^-1, respectively, much higher than that in suspended cultivation system. The results shows that T. minus can be cultured with attached cultivation method to improve its biomass productivity.展开更多
A novel compound, plumbocatechin A 1, was isolated from the whole plant of Ceratostigma minus. The structural elucidation of 1 was mainly achieved by 2D-NMR techniques.
AIM： To find out whether there is a significant difference in the prevalence of the precore stop codon mutation between HBeAg positive and anti-HBe positive children. METHODS： We investigated a large pediatric popul...AIM： To find out whether there is a significant difference in the prevalence of the precore stop codon mutation between HBeAg positive and anti-HBe positive children. METHODS： We investigated a large pediatric population of 155 European children （mean age 10.9 years） with chronic hepatitis B by PCR and direct sequencing. Ninety were HBeAg positive and 65 had seroconversion to anti-HBe. Additionally genotyping was performed. RESULTS： Seventy-four （48%） of the sequenced HBV strains were attributed to genotype D and 81 （52%） to genotype A. In the group of 90 HBeAg positive patients, 2 （2.2%） 1896-G-to-A transitions leading to precore stop codon mutation were found, and in the group of 65 anti-HBe positive children, 5 （7.7%） were identified harbouring HBeAg-minus mutants. The difference was not statistically significant （P= 0.13）. CONCLUSIONS： HBeAg minus variants as predominant viral HB strains play a minor role in the course of chronic hepatitis B in European children.展开更多
Busbar Automatic Transfer Switch (BATS) is very important for power distribution reliability. However, BATS can’t consider whether it cause overloading of devices after it acts. In this paper, we introduce the design...Busbar Automatic Transfer Switch (BATS) is very important for power distribution reliability. However, BATS can’t consider whether it cause overloading of devices after it acts. In this paper, we introduce the design principle of Automatic BATS control from whole architecture including its function, strategy and a rule of on-off. On the other hand, the running experience and effect is also introduced. Site operation shows that the proposed method is feasible and it can ensure power grid reliability.展开更多
Objective: To explore the protective effect of Polygonum minus ethanolic extract on cisplatininduced neurotoxicity.Methods: In vitro test, total phenolic content assay and DPPH assay were performed to determine the an...Objective: To explore the protective effect of Polygonum minus ethanolic extract on cisplatininduced neurotoxicity.Methods: In vitro test, total phenolic content assay and DPPH assay were performed to determine the antioxidant activity of Polygonum minus. For in vivo test, 30 male SpragueDawley rats were randomly divided into 5 groups: the control group, cisplatin 10 mg/kg, Polygonum minus 100 mg/kg, Polygonum minus 200 mg/kg and Polygonum minus 400 mg/kg. The control group and the cisplatin group were given distilled water whereas Polygonum minus groups received the respective dose of Polygonum minus extract orally for 14 d. On day 15, a single intraperitoneal administration of normal saline was given to the control group; while 10 mg/kg of cisplatin was given to the cisplatin group and Polygonum minus groups. Body weight, signs of illness, daily activity and mortality were observed at least once daily throughout the experimental period. On day 18, the anterior part of the brain was collected and processed for histological and ultrastructural analyses(right hemisphere). The remaining part(left hemisphere) of the brain was assayed to determine malondialdehyde and catalase levels for oxidative stress analyses.Results: Polygonum minus ethanolic extract possessed high phenolic content(977.6 mg GAE/g) and 95.9% DPPH radical scavenging activities. No mortality was observed in all groups. Rats in the cisplatin group were weak and less active compared to Polygonum minus treated rats. In the cisplatin group, disorganised cellular layers of the cerebral cortex were observed whereas rats treated with low and mid doses of Polygonum minus extract had normal cerebral cortex as in the control group. Mild ultrastructural changes were observed in rats treated with low and mid doses of Polygonum minus extract. Meanwhile, low and mid doses of Polygonum minus extract significantly reduced malondialdehyde level whereas low and mid doses of Polygonum minus extracts groups significantly increased catalase activity compared to the cisp展开更多
Let G=(V,E)be a graph,for an element x∈V∪E,the open total neighborhood of x is denoted by N_(t)(x)={y|y is adjacent to x or y is incident with x,y∈V∪E},and Nt[x]=Nt(x)∪{x}is the closed one.A function f:V(G)∪E(G...Let G=(V,E)be a graph,for an element x∈V∪E,the open total neighborhood of x is denoted by N_(t)(x)={y|y is adjacent to x or y is incident with x,y∈V∪E},and Nt[x]=Nt(x)∪{x}is the closed one.A function f:V(G)∪E(G)→{−1,0,1}is said to be a mixed minus domination function(TMDF)of G if∑_(y∈Nt[x])f(y)≥1 holds for all x∈V(G)∪E(G).The mixed minus domination numberγ′_(tm)(G)of G is defined as γ′_(tm)(G)=min{∑x∈V∪E f(x)|f is a TMDF of G.In this paper,we obtain some lower bounds of the mixed minus domination number of G and give the exact values ofγ′_(tm)(G)when G is a cycle or a path.展开更多
I. INTRODUCTION A ring R is called a regular ring if a∈aRa, for each a∈R. A solution to axa=a is called an inner inverse of a ,denoted by a^-. A solution to xax=x is called an outer inverse of a. A solution to both ...I. INTRODUCTION A ring R is called a regular ring if a∈aRa, for each a∈R. A solution to axa=a is called an inner inverse of a ,denoted by a^-. A solution to xax=x is called an outer inverse of a. A solution to both axa=a and xax=x is called a reflexive inverse of a, and denoted by a^+. Inner and reflexive inverses of an element are not unique in general. The sets of all in-展开更多
基金Supported by the National Science Foundation of Jiangxi province(9911020).
文摘In this paper we obtain some lower bounds for minus and signed domination numbers. We also prove and generalize a conjecture on the minus domination number for bipartite graph of order n, which was proposed by Jean Dunbar et al [1].
文摘基于经过非均一性检验及订正前后1979-2003年中国东部地区130个气象台站的日平均、最高和最低温度常规观测资料,利用研究区域内气象台站常规观测资料和NCEP/NCAR(The NationalCenters for Atmospheric Prediction and the National Center for Atmosphetic Research)再分析资料温度变化趋势的差值,本文定量考察了研究区域内城市化及土地利用类型改变对区域温度气候趋势的贡献.研究结果显示,经过订正后的台站观测数据具有一定的可信度,比订正前更具合理性.城市化及土地利用类型的改变造成了研究区域内城镇气象站点日平均、最高和最低温度的上升,其中日最低温度的上升程度大于日最高温度,从而导致了日较差温度的下降.进一步研究表明,从1980年代后期开始,城镇气象站点的日平均和最低温度明显上升.从不同季节来看,冬季是区域温度变化趋势最为明显的季节,但却是城市化及土地利用类型改变对区域温度气候趋势贡献最小的季节,对研究区域温度气候趋势贡献最大的季节为夏季.
文摘The study of minus paired domination of a graph G=(V,E) is initiated. Let SV be any paired dominating set of G , a minus paired dominating function is a function of the form f∶V→{-1,0,1} such that f(v)= 1 for v∈S, f(v)≤0 for v∈V-S , and f(N)≥1 for all v∈V . The weight of a minus paired dominating function f is w(f)=∑f(v) , over all vertices v∈V . The minus paired domination number of a graph G is γ - p( G )=min{ w(f)|f is a minus paired dominating function of G }. On the basis of the minus paired domination number of a graph G defined, some of its properties are discussed.
基金Project supported by the National Natural Science Foundation of China （Grant No.10571117）, the Shuguang Plan of Shang- hai Education Devel0pment Foundation （Grant No.06SG42）, and the Natural Science Development Foundation of Shanghai Municipal Commission of Education （Grant No.05AZ04）
文摘For an arbitrary subset P of the reals, a function f ： V →P is defined to be a P-dominating function of a graph G = （V, E） if the sum of its function values over any closed neighbourhood is at least 1. That is, for every v ∈ V, f（N[v]） ≥ 1. The definition of total P-dominating function is obtained by simply changing ‘closed＇ neighborhood N[v] in the definition of P-dominating function to ‘open＇ neighborhood N（v）. The （total） P-domination number of a graph G is defined to be the infimum of weight w（f） = ∑v ∈ V f（v） taken over all （total） P-dominating function f. Similarly, the P-edge and P-star dominating functions can be defined. In this paper we survey some recent progress on the topic of dominating functions in graph theory. Especially, we are interested in P-, P-edge and P-star dominating functions of graphs with integer values.
基金supported by grants from the Ministry of Science and Technology of China and the National Natural Science Foundation of China(2022YFA1303100,32090040,92254302,2022YFA0806800,91854203,31621002,2017YFA0503600,21922706,and 92153302 to Xing Liu,2022YFA1302700 to Z.W.,32100612 to Xu Liu)the Ministry of Education of China(IRT_17R102,20113402130010,and YD2070006001 to Xing Liu)+3 种基金the Plans for Major Provincial Science&Technology Projects of Anhui Province(202303a0702003 to Xing Liu)the Fundamental Research Funds for the Central Universities(KB9100000007 and KB9100000013 to Xing Liu)University of Science and Technology of China Start-up Fund(KY9990000167 to Z.W.)Zhejiang Provincial Natural Science Foundation(LY23C070002 to W.W.)。
文摘Although the dynamic instability of microtubules(MTs)is fundamental to many cellular functions,quiescent MTs with unattached free distal ends are commonly present and play important roles in various events to power cellular dynamics.However,how these free MT tips are stabilized remains poorly understood.Here,we report that centrosome and spindle pole protein 1(CSPP1)caps and stabilizes both plus and minus ends of static MTs.Real-time imaging of laser-ablated MTs in live cells showed deposition of CSPP1 at the newly generated MT ends,whose dynamic instability was concomitantly suppressed.Consistently,MT ends in CSPP1-overexpressing cells were hyper-stabilized,while those in CSPP1-depleted cells were much more dynamic.This CSPP1-elicited stabilization of MTs was demonstrated to be achieved by suppressing intrinsic MT catastrophe and restricting polymerization.Importantly,CSPP1-bound MTs were resistant to mitotic centromere-associated kinesin-mediated depolymerization.These findings delineate a previously uncharacterized CSPP1 activity that integrates MT end capping to orchestrate quiescent MTs.
基金supported by the Strategic Priority Research Program of the Chinese Academy of Sciences(XDA19030204)the CAS"Light of West China"Program(2015-XBQNB-17)
文摘The surface wind speed(SWS)is affected by both large-scale circulation and land use and cover change(LUCC).In China,most studies have considered the effect of large-scale circulation rather than LUCC on SWS.In this study,we evaluated the effects of LUCC on the SWS decrease during 1979-2015 over China using the observation minus reanalysis(OMR)method.There were two key findings:(1)Observed wind speed declined significantly at a rate of 0.0112 m/(s·a),whereas ERA-Interim,which can only capture the inter-annual variation of observed data,indicated a gentle downward trend.The effects of LUCC on SWS were distinct and caused a decrease of 0.0124 m/(s·a)in SWS;(2)Due to variations in the characteristics of land use types across different regions,the influence of LUCC on SWS also varied.The observed wind speed showed a rapid decline over cultivated land in Northwest China,as well as a decrease in China’s northeastern and eastern plain regions due to the urbanization.However,in the Tibetan Plateau,the impact of LUCC on wind speed was only slight and can thus be ignored.
文摘The CTD (conductivity, temperature and depth) data collected by six China-Korea joint cruises during 1996-1998 and the climatological data suggest that the seasonal variability of average salinity in the Yellow Sea (Sa) presents a general sinusoid pattern. To study the mechanism of the variability, annual cycles of Sa were simulated and a theoretical analysis based on the governing equations was reported.Three main factors are responsible for the variability: the Yellow Sea Warm Current (YSWC), the Changji-ang (Yangtze) River diluted water (YRDW) and the evaporation minus precipitation (E-P). From December to the next May, the variability of Sa is mainly controlled by the salt transportation of the YSWC. But in early July, the YSWC is overtaken and replaced by the YRDW which then becomes the most important controller in summer. From late September to November, the E-P gradually took the lead. The mass exchange north of the 37癗 line is not significant.
文摘AIM： We designed two synthetic-core-specific peptides core 1 （C1） and core 2 （C2）, and an E1-specific peptide （El）. We produced specific polyclonal antibodies against these peptides and used the antibodies for detection of HCV antigens on surface and within infected peripheral blood leukocytes. METHODS： Peripheral blood from a healthy individual who tested negative for HCV RNA was incubated with HCV type 4 infected serum for i h and 24 h at 37 ℃. Cells were stained by direct and indirect immunofluorescence and measured by flow cytometry. RESULTS： After 1 h of incubation, antibodies against C1, C2, and El detected HCV antigens on the surface of 27%, 26% and 73% of monocytes respectively, while 10%, 5% and 9% of lymphocytes were positive with anti-C1, anti-C2 and anti-E1 respectively. Only 1-3% of granulocytes showed positive staining with anti-C1, anti-C2 and anti E1 antibodies. After 24 h of incubation, we found no surface staining with anti-C1, anti-C2 or anti-E1. Direct immunostaining using anti-C2 could not detect intracellular HCV antigens, after 1 h of incubation with the virus, while after 24 h of incubation, 28% of infected cells showed positive staining. Only plus strand RNA was detectable intracellularly as early as 1 h after incubation, and remained detectable throughout 48 h post-infection. Interestingly, minus RNA strand could not be detected after 1 h, but became strongly detectable intracellularly after 24 h post-infection. CONCLUSION： Monocytes and lymphocytes are the preferred target cells for HCV infection in peripheral blood leukocytes. Our specific anti-core and anti-E1 antibodies are valuable reagents for demonstration of HCV cell cycle. Also, HCV is capable of infecting and replicating in peripheral blood mononuclear cells as confirmed by detection of minus strand HCV RNA as well as intracellular staining of core HCV antigen.
文摘An upper bound is established on the parameter Γ -(G) for a cubic graph G and two infinite families of 3-connected graphs G k, G * k are constructed to show that the bound is sharp and, moreover, the difference Γ -(G * k)-γ s(G * k) can be arbitrarily large, where Г -(G * k) and γ s(G * k) are the upper minus domination and signed domination numbers of G * k, respectively. Thus two open problems are solved.
基金supported by the Strategic Priority Research Program of Chinese Academy of Sciences (Transformational Technologies for Clean Energy and Demonstration)(No.XDA21010211)the Shandong Provincial Natural Science Foundation (No.ZR2017QC007)the Youth Innovation Promotion Association, CAS。
文摘Attached cultivation is a promising method for microalgal biomass production. Filamentous oleaginous microalga Tribonema minus(hereafter T. minus) has shown a remarkable potential for biofuel production in terms of its high lipid content. However, the strain has only been cultivated in suspended cultivation systems including open pond and closed photobioreactors. Here, we attempted to study the attached cultivation of T. minus, which might be helpful for its scale-up cultivation and industrial applications. As the results, the optimal conditions for T. minus growth in the attached biofilm are 200 μmol photons m^-2 s^-1 of light intensity and 5% of CO2, and the maximum biomass density of 223 g m^-2 has been achieved under the light intensity. The non-woven fabric as substratum was found as the best substratum in thin layer attached bioreactor, on which the average biomass productivity of T. minus is about(9.73 ± 2.19) g m^-2)d^-1. Furthermore, two attached bioreactor systems, rotary drum and rotation disc, were designed following the light dilution strategy and introduced into T. minus cultivation. The highest footprint areal biomass productivity of these two systems is 33 and 47.1 g m^-2 d^-1, respectively, much higher than that in suspended cultivation system. The results shows that T. minus can be cultured with attached cultivation method to improve its biomass productivity.
文摘A novel compound, plumbocatechin A 1, was isolated from the whole plant of Ceratostigma minus. The structural elucidation of 1 was mainly achieved by 2D-NMR techniques.
文摘AIM： To find out whether there is a significant difference in the prevalence of the precore stop codon mutation between HBeAg positive and anti-HBe positive children. METHODS： We investigated a large pediatric population of 155 European children （mean age 10.9 years） with chronic hepatitis B by PCR and direct sequencing. Ninety were HBeAg positive and 65 had seroconversion to anti-HBe. Additionally genotyping was performed. RESULTS： Seventy-four （48%） of the sequenced HBV strains were attributed to genotype D and 81 （52%） to genotype A. In the group of 90 HBeAg positive patients, 2 （2.2%） 1896-G-to-A transitions leading to precore stop codon mutation were found, and in the group of 65 anti-HBe positive children, 5 （7.7%） were identified harbouring HBeAg-minus mutants. The difference was not statistically significant （P= 0.13）. CONCLUSIONS： HBeAg minus variants as predominant viral HB strains play a minor role in the course of chronic hepatitis B in European children.
文摘Busbar Automatic Transfer Switch (BATS) is very important for power distribution reliability. However, BATS can’t consider whether it cause overloading of devices after it acts. In this paper, we introduce the design principle of Automatic BATS control from whole architecture including its function, strategy and a rule of on-off. On the other hand, the running experience and effect is also introduced. Site operation shows that the proposed method is feasible and it can ensure power grid reliability.
文摘Objective: To explore the protective effect of Polygonum minus ethanolic extract on cisplatininduced neurotoxicity.Methods: In vitro test, total phenolic content assay and DPPH assay were performed to determine the antioxidant activity of Polygonum minus. For in vivo test, 30 male SpragueDawley rats were randomly divided into 5 groups: the control group, cisplatin 10 mg/kg, Polygonum minus 100 mg/kg, Polygonum minus 200 mg/kg and Polygonum minus 400 mg/kg. The control group and the cisplatin group were given distilled water whereas Polygonum minus groups received the respective dose of Polygonum minus extract orally for 14 d. On day 15, a single intraperitoneal administration of normal saline was given to the control group; while 10 mg/kg of cisplatin was given to the cisplatin group and Polygonum minus groups. Body weight, signs of illness, daily activity and mortality were observed at least once daily throughout the experimental period. On day 18, the anterior part of the brain was collected and processed for histological and ultrastructural analyses(right hemisphere). The remaining part(left hemisphere) of the brain was assayed to determine malondialdehyde and catalase levels for oxidative stress analyses.Results: Polygonum minus ethanolic extract possessed high phenolic content(977.6 mg GAE/g) and 95.9% DPPH radical scavenging activities. No mortality was observed in all groups. Rats in the cisplatin group were weak and less active compared to Polygonum minus treated rats. In the cisplatin group, disorganised cellular layers of the cerebral cortex were observed whereas rats treated with low and mid doses of Polygonum minus extract had normal cerebral cortex as in the control group. Mild ultrastructural changes were observed in rats treated with low and mid doses of Polygonum minus extract. Meanwhile, low and mid doses of Polygonum minus extract significantly reduced malondialdehyde level whereas low and mid doses of Polygonum minus extracts groups significantly increased catalase activity compared to the cisp
基金This work was supported by the National Natural Science Foundation of China(No.11061014,11361024,11261019)the Science Foundation of Jiangxi Province(No.KJLD12067)The authors are grateful to the referees for their careful reading with corrections and especially the referee who draws our attention to the proof in Theorem 2.2,which let us improve the proof of Theorem 2.2,and correct this lower bound.
文摘Let G=(V,E)be a graph,for an element x∈V∪E,the open total neighborhood of x is denoted by N_(t)(x)={y|y is adjacent to x or y is incident with x,y∈V∪E},and Nt[x]=Nt(x)∪{x}is the closed one.A function f:V(G)∪E(G)→{−1,0,1}is said to be a mixed minus domination function(TMDF)of G if∑_(y∈Nt[x])f(y)≥1 holds for all x∈V(G)∪E(G).The mixed minus domination numberγ′_(tm)(G)of G is defined as γ′_(tm)(G)=min{∑x∈V∪E f(x)|f is a TMDF of G.In this paper,we obtain some lower bounds of the mixed minus domination number of G and give the exact values ofγ′_(tm)(G)when G is a cycle or a path.
文摘I. INTRODUCTION A ring R is called a regular ring if a∈aRa, for each a∈R. A solution to axa=a is called an inner inverse of a ,denoted by a^-. A solution to xax=x is called an outer inverse of a. A solution to both axa=a and xax=x is called a reflexive inverse of a, and denoted by a^+. Inner and reflexive inverses of an element are not unique in general. The sets of all in-
