With the occurrence of aging process,decreased neuron dopamine,disrupted brown adipose tissue(BAT)remodeling and decreased butyrate level all reflect a weak host healthy in certain degree.Nevertheless,the signs of mid...With the occurrence of aging process,decreased neuron dopamine,disrupted brown adipose tissue(BAT)remodeling and decreased butyrate level all reflect a weak host healthy in certain degree.Nevertheless,the signs of mid-adult gut microbiota,and its association with host healthy are not well understood.In current study,we deemed to illustrate the associations of age,neuron dopamine,BAT remodeling,butyrate and gut microbiota with the aid of traditional herbal formula Kang Shuai Lao Pian(KSLP),which is known for its anti-aging effect.Here,ELISA was performed to detect the production of brain dopamine,the mass of inguinal white adipose tissue versus interscapular brown adipose tissue(iWAT/iBAT)was calculated and considered as a sign of BAT remodeling,16 S rRNA gene sequencing was used to the detection of gut microbiota profiling and gas chromatography was used to measure the butyrate level in mice feces.Our results indicated mid-adult mice already present distinctive gut microbiota profiling compared with young mice,concomitant with which are the lower brain dopamine level and disrupted brown adipose remodeling.KSLP treatment improved the host healthy and regulated gut microbiota with enriched Firmicutes at the expense of Bacteroidetes,particularly increased the relative abundance of bacteria functionally related to dopamine and butyrate productions,which suggest KSLP treatment constructs a healthier gut environment.In conclusion,modulation of gut microbiota and butyrate may connectively regulate dopamine production and BAT remodeling through gut-brain axis and gut-metabolism axis.展开更多
研究根据"时间意识"的理论及已有实证结论,通过访谈法和问卷法,探讨"时间流逝感"(feeling of the passage of time,FPT)这一概念的内部结构。结果发现,探索性因子分析提取出时间流逝感的三个子维度,即"情绪情...研究根据"时间意识"的理论及已有实证结论,通过访谈法和问卷法,探讨"时间流逝感"(feeling of the passage of time,FPT)这一概念的内部结构。结果发现,探索性因子分析提取出时间流逝感的三个子维度,即"情绪情感"、"易感性"和"行为反应倾向"。前两个维度验证了前人"情绪—注意"的理论模型,而"行为反应倾向"则是本研究的新发现之一,这在某种程度上丰富了时间流逝感的概念内涵。验证性因子分析显示该量表的三维结构在不同人群中(中学生、大学生、中年人)存在较为稳定的一致性。该量表总分及其三个维度与焦虑、抑郁量表呈现出显著相关,故FPT量表具有一定的效标关联效度。该量表的分半信度、组合信度和内部一致性信度符合计量心理学的基本要求。最后,文章基于FFT信效度分析中存在的问题及优化策略予以了讨论。结论:本研究对时间流逝感的三维结构进行了初步探讨,具有一定的信效度,可作为测量不同人群有关时间流逝态度与情感的一般工具。展开更多
基金supported by the Science and Technology Department of Zhejiang Province,China(Nos.2018C02048 and 2018F10076)the Agricultural and Social Development Department of Hangzhou City,China(2018)。
文摘With the occurrence of aging process,decreased neuron dopamine,disrupted brown adipose tissue(BAT)remodeling and decreased butyrate level all reflect a weak host healthy in certain degree.Nevertheless,the signs of mid-adult gut microbiota,and its association with host healthy are not well understood.In current study,we deemed to illustrate the associations of age,neuron dopamine,BAT remodeling,butyrate and gut microbiota with the aid of traditional herbal formula Kang Shuai Lao Pian(KSLP),which is known for its anti-aging effect.Here,ELISA was performed to detect the production of brain dopamine,the mass of inguinal white adipose tissue versus interscapular brown adipose tissue(iWAT/iBAT)was calculated and considered as a sign of BAT remodeling,16 S rRNA gene sequencing was used to the detection of gut microbiota profiling and gas chromatography was used to measure the butyrate level in mice feces.Our results indicated mid-adult mice already present distinctive gut microbiota profiling compared with young mice,concomitant with which are the lower brain dopamine level and disrupted brown adipose remodeling.KSLP treatment improved the host healthy and regulated gut microbiota with enriched Firmicutes at the expense of Bacteroidetes,particularly increased the relative abundance of bacteria functionally related to dopamine and butyrate productions,which suggest KSLP treatment constructs a healthier gut environment.In conclusion,modulation of gut microbiota and butyrate may connectively regulate dopamine production and BAT remodeling through gut-brain axis and gut-metabolism axis.
文摘研究根据"时间意识"的理论及已有实证结论,通过访谈法和问卷法,探讨"时间流逝感"(feeling of the passage of time,FPT)这一概念的内部结构。结果发现,探索性因子分析提取出时间流逝感的三个子维度,即"情绪情感"、"易感性"和"行为反应倾向"。前两个维度验证了前人"情绪—注意"的理论模型,而"行为反应倾向"则是本研究的新发现之一,这在某种程度上丰富了时间流逝感的概念内涵。验证性因子分析显示该量表的三维结构在不同人群中(中学生、大学生、中年人)存在较为稳定的一致性。该量表总分及其三个维度与焦虑、抑郁量表呈现出显著相关,故FPT量表具有一定的效标关联效度。该量表的分半信度、组合信度和内部一致性信度符合计量心理学的基本要求。最后,文章基于FFT信效度分析中存在的问题及优化策略予以了讨论。结论:本研究对时间流逝感的三维结构进行了初步探讨,具有一定的信效度,可作为测量不同人群有关时间流逝态度与情感的一般工具。
