目的通过研究噪声暴露致小鼠听力损伤后,耳蜗背侧核的兴奋性梭形神经元和耳蜗腹侧核的抑制性D型星形神经元接受听觉神经输入的电生理特性改变,探索噪声致听觉系统外周损伤后引起的中枢神经通路的变化。方法30只健康成年的转基因小鼠SOM-...目的通过研究噪声暴露致小鼠听力损伤后,耳蜗背侧核的兴奋性梭形神经元和耳蜗腹侧核的抑制性D型星形神经元接受听觉神经输入的电生理特性改变,探索噪声致听觉系统外周损伤后引起的中枢神经通路的变化。方法30只健康成年的转基因小鼠SOM-tdTomato采用随机数字表法随机分为对照组(15只,不给予任何噪声暴露)、噪声暴露组(15只,120 dB SPL白噪声每天暴露4 h,连续暴露7 d)。应用听性脑干反应(ABR)测试两组小鼠的听功能,应用膜片钳离体脑片技术分析比较两组小鼠听觉神经到耳蜗核的梭形神经元和D型星形神经元投射通路电生理特性的改变;应用免疫荧光染色法确定记录神经元的类型。结果与对照组比较,噪声暴露组小鼠短声和短纯音ABR反应阈值升高,波Ⅲ潜伏期延长(P<0.05)。膜片钳离体脑片记录结果表明:两类神经元都表现有来自听神经的兴奋性突触后反应,与对照组比较,噪声暴露组反应幅度降低、概率下降、延时延长,其中对D型星形神经元的支配效率降低更多。结论听觉神经可以直接支配耳蜗核的梭形神经元和D型星形神经元;噪声损伤听力后,这种支配效率和强度均明显降低。展开更多
Positive type photosensitive polyimide is used as the modification layer in the thin film transistors production process. The photosensitive polyimide is not only used as the second insulating layer, it can also be us...Positive type photosensitive polyimide is used as the modification layer in the thin film transistors production process. The photosensitive polyimide is not only used as the second insulating layer, it can also be used instead of a mask because of the photosensitivity. A suitable curing condition can help photosensitive polyimide form the high performance polyimide with orderly texture inside, and the performance of imidization depends on the precise control of temperature, time, and heat control during the curing process. Therefore, experiments of different stepped up heating tests are made, and the ability of protecting silicon dioxide is analyzed.展开更多
Extremely low-frequency magnetic field is widely used as a noninvasive stimulation method in clinical practice and basic research. Electrical field induced from magnetic pulse can decrease or increase neuronal electri...Extremely low-frequency magnetic field is widely used as a noninvasive stimulation method in clinical practice and basic research. Electrical field induced from magnetic pulse can decrease or increase neuronal electrical activity. However, the cellular mechanism underlying the effects of magnetic field is not clear from experimental data. Recent studies have demonstrated that "non-neuronal" cells, especially astrocytes, may be the potential effector for transcranial magnetic stimulation(TMS). In the present study, we implemented a neural–astrocyte microcircuit computational model based on hippocampal architecture to investigate the biological effects of different magnetic field frequencies on cells. The purpose of the present study is to elucidate the main influencing factors of MS to allow a better understanding of its mechanisms.Our model reproduced the basic characteristics of the neuron and astrocyte response to different magnetic stimulation. The results predict that interneurons with lower firing thresholds were more active in magnetic fields by contrast to pyramidal neurons. And the synaptic coupling strength between the connected neurons may be one of the critical factor to affect the effect of magnetic field on cells. In addition, the simulations show that astrocytes can decrease or increase slow inward currents(SICs) to finely tune neuronal excitation, which suggests their key role in excitatory–inhibitory balance. The interaction between neurons and astrocytes may represent a novel target for effective therapeutic strategies involving magnetic stimulation.展开更多
根据我国海洋综合气象监测装备建设网点不继扩大和加密的现状,依靠现有的卫星通信网络,利用其覆盖范围广、通信稳定、实时在线和维持经费等优势,设计一种基于卫星通信网络的海洋气象数据采集系统。介绍该系统的数据采集结构和功能,智能...根据我国海洋综合气象监测装备建设网点不继扩大和加密的现状,依靠现有的卫星通信网络,利用其覆盖范围广、通信稳定、实时在线和维持经费等优势,设计一种基于卫星通信网络的海洋气象数据采集系统。介绍该系统的数据采集结构和功能,智能处理终端的硬件组成,硬件电路芯片选型与使用;介绍系统软件的控制和编制方法,简述在可视化编程语言Microsoft Visual Studio 6.0集成包环境下开发海洋气象数据采集系统软件的步骤;讨论基于我国北斗卫星通信网络的海洋气象数据采集系统的实际应用情况。展开更多
根据我国实时监测气象设备布设网点不断扩大和加密的现状,提出利用现有的GPRS网络覆盖范围广、实时在线、通信质量高和维持费用低等优势,设计一种基于GPRS网络的远程气象数据采集系统。介绍该系统的结构模式和功能,系统中远程测控终端...根据我国实时监测气象设备布设网点不断扩大和加密的现状,提出利用现有的GPRS网络覆盖范围广、实时在线、通信质量高和维持费用低等优势,设计一种基于GPRS网络的远程气象数据采集系统。介绍该系统的结构模式和功能,系统中远程测控终端的硬件结构组成,相关的电路芯片使用。阐述编制相应控制软件的方法,在Microsoft Visual Studio 6.0可视化编程语言集成包环境下开发管理中心软件的方法,以及基于GPRS网络的远程气象数据采集系统的实际应用情况。展开更多
文摘目的通过研究噪声暴露致小鼠听力损伤后,耳蜗背侧核的兴奋性梭形神经元和耳蜗腹侧核的抑制性D型星形神经元接受听觉神经输入的电生理特性改变,探索噪声致听觉系统外周损伤后引起的中枢神经通路的变化。方法30只健康成年的转基因小鼠SOM-tdTomato采用随机数字表法随机分为对照组(15只,不给予任何噪声暴露)、噪声暴露组(15只,120 dB SPL白噪声每天暴露4 h,连续暴露7 d)。应用听性脑干反应(ABR)测试两组小鼠的听功能,应用膜片钳离体脑片技术分析比较两组小鼠听觉神经到耳蜗核的梭形神经元和D型星形神经元投射通路电生理特性的改变;应用免疫荧光染色法确定记录神经元的类型。结果与对照组比较,噪声暴露组小鼠短声和短纯音ABR反应阈值升高,波Ⅲ潜伏期延长(P<0.05)。膜片钳离体脑片记录结果表明:两类神经元都表现有来自听神经的兴奋性突触后反应,与对照组比较,噪声暴露组反应幅度降低、概率下降、延时延长,其中对D型星形神经元的支配效率降低更多。结论听觉神经可以直接支配耳蜗核的梭形神经元和D型星形神经元;噪声损伤听力后,这种支配效率和强度均明显降低。
文摘Positive type photosensitive polyimide is used as the modification layer in the thin film transistors production process. The photosensitive polyimide is not only used as the second insulating layer, it can also be used instead of a mask because of the photosensitivity. A suitable curing condition can help photosensitive polyimide form the high performance polyimide with orderly texture inside, and the performance of imidization depends on the precise control of temperature, time, and heat control during the curing process. Therefore, experiments of different stepped up heating tests are made, and the ability of protecting silicon dioxide is analyzed.
基金supported by the National Natural Science Foundation of China (Grant No. 61673158)the Youth Talent Support Program of Hebei Province,China(Grant No. BJ2019044)。
文摘Extremely low-frequency magnetic field is widely used as a noninvasive stimulation method in clinical practice and basic research. Electrical field induced from magnetic pulse can decrease or increase neuronal electrical activity. However, the cellular mechanism underlying the effects of magnetic field is not clear from experimental data. Recent studies have demonstrated that "non-neuronal" cells, especially astrocytes, may be the potential effector for transcranial magnetic stimulation(TMS). In the present study, we implemented a neural–astrocyte microcircuit computational model based on hippocampal architecture to investigate the biological effects of different magnetic field frequencies on cells. The purpose of the present study is to elucidate the main influencing factors of MS to allow a better understanding of its mechanisms.Our model reproduced the basic characteristics of the neuron and astrocyte response to different magnetic stimulation. The results predict that interneurons with lower firing thresholds were more active in magnetic fields by contrast to pyramidal neurons. And the synaptic coupling strength between the connected neurons may be one of the critical factor to affect the effect of magnetic field on cells. In addition, the simulations show that astrocytes can decrease or increase slow inward currents(SICs) to finely tune neuronal excitation, which suggests their key role in excitatory–inhibitory balance. The interaction between neurons and astrocytes may represent a novel target for effective therapeutic strategies involving magnetic stimulation.
文摘根据我国海洋综合气象监测装备建设网点不继扩大和加密的现状,依靠现有的卫星通信网络,利用其覆盖范围广、通信稳定、实时在线和维持经费等优势,设计一种基于卫星通信网络的海洋气象数据采集系统。介绍该系统的数据采集结构和功能,智能处理终端的硬件组成,硬件电路芯片选型与使用;介绍系统软件的控制和编制方法,简述在可视化编程语言Microsoft Visual Studio 6.0集成包环境下开发海洋气象数据采集系统软件的步骤;讨论基于我国北斗卫星通信网络的海洋气象数据采集系统的实际应用情况。
文摘根据我国实时监测气象设备布设网点不断扩大和加密的现状,提出利用现有的GPRS网络覆盖范围广、实时在线、通信质量高和维持费用低等优势,设计一种基于GPRS网络的远程气象数据采集系统。介绍该系统的结构模式和功能,系统中远程测控终端的硬件结构组成,相关的电路芯片使用。阐述编制相应控制软件的方法,在Microsoft Visual Studio 6.0可视化编程语言集成包环境下开发管理中心软件的方法,以及基于GPRS网络的远程气象数据采集系统的实际应用情况。
