Exploring the transition from inter-ictal to ictal epileptiform discharges(IDs) and how GABAAreceptormediated action affects the onset of IDs will enrich our understanding of epileptogenesis and epilepsy treatment.We ...Exploring the transition from inter-ictal to ictal epileptiform discharges(IDs) and how GABAAreceptormediated action affects the onset of IDs will enrich our understanding of epileptogenesis and epilepsy treatment.We used Mg2+-free artificial cerebrospinal fluid(ACSF) to induce epileptiform discharges in juvenile mouse hippocampal slices and used a micro-electrode array to record the discharges. After the slices were exposed to Mg2+-free ACSF for 10 min–20 min, synchronous recurrent seizurelike events were recorded across the slices, and each event evolved from inter-ictal epileptiform discharges(IIDs) to pre-ictal epileptiform discharges(PIDs), and then to IDs.During the transition from IIDs to PIDs, the duration of discharges increased and the inter-discharge interval decreased. After adding 3 lmol/L of the GABAAreceptor agonist muscimol, PIDs and IDs disappeared, and IIDs remained. Further, the application of 10 lmol/L muscimol abolished all the epileptiform discharges. When the GABAAreceptor antagonist bicuculline was applied at 10 lmol/L, IIDs and PIDs disappeared, and IDs remained at decreased intervals. These results indicated that there are dynamic changes in the hippocampal network preceding the onset of IDs, and GABAAreceptor activity suppresses the transition from IIDs to IDs in juvenile mouse hippocampus.展开更多
Cu films were electrodeposited under a magnetic field of 5 T on the Pt disc electrodes with diameters of 3 mm ~ 25 μm.Such magnetoelectrodeposited(MED) Cu films were employed as an electrode,and cyclic voltammograms ...Cu films were electrodeposited under a magnetic field of 5 T on the Pt disc electrodes with diameters of 3 mm ~ 25 μm.Such magnetoelectrodeposited(MED) Cu films were employed as an electrode,and cyclic voltammograms were measured for the electrochemical reactions of an amino acid of alanine.Chiral behavior was observed as oxidation current difference between the enantiomers.Clear magnetoelectrochemical chirality was observed on the micro-disk electrode and on the kinetic-controlled electrode process of alanine.The chiral response was durable for more than 10 cycles of the consecutive voltammetric measurements of the enantiomers.展开更多
Recent work in the field ofneurophysiology has demonstrated that, by observing the firing characteristic of action potentials （AP） and the exchange pattern of signals between neurons, it is possible to reveal the na...Recent work in the field ofneurophysiology has demonstrated that, by observing the firing characteristic of action potentials （AP） and the exchange pattern of signals between neurons, it is possible to reveal the nature of ＂memory＂ and ＂thinking＂ and help humans to understand how the brain works. To address these needs, we developed a prototype fully integrated circuit （IC） with micro-electrode array （MEA） for neural recording. In this scheme, the microelectrode array is composed by 64 detection electrodes and 2 reference electrodes. The proposed IC consists of 8 recording channels with an area of 5 x 5 mm2. Each channel can operate independently to process the neural signal by amplifying, filtering, etc. The chip is fabricated in 0.5-#m CMOS technology. The simulated and measured results show the system provides an effective device for recording feeble signal such as neural signals.展开更多
基金supported by the Key Basic Research Project of Science and Technology Commission of Shanghai (13DJ1400303)the Shanghai Jiao Tong University Fund for Interdisciplinary Research for Medical Applications (YG2012ZD08)the Seed Fund of Ren Ji Hospital (RJ ZZ13-005)
文摘Exploring the transition from inter-ictal to ictal epileptiform discharges(IDs) and how GABAAreceptormediated action affects the onset of IDs will enrich our understanding of epileptogenesis and epilepsy treatment.We used Mg2+-free artificial cerebrospinal fluid(ACSF) to induce epileptiform discharges in juvenile mouse hippocampal slices and used a micro-electrode array to record the discharges. After the slices were exposed to Mg2+-free ACSF for 10 min–20 min, synchronous recurrent seizurelike events were recorded across the slices, and each event evolved from inter-ictal epileptiform discharges(IIDs) to pre-ictal epileptiform discharges(PIDs), and then to IDs.During the transition from IIDs to PIDs, the duration of discharges increased and the inter-discharge interval decreased. After adding 3 lmol/L of the GABAAreceptor agonist muscimol, PIDs and IDs disappeared, and IIDs remained. Further, the application of 10 lmol/L muscimol abolished all the epileptiform discharges. When the GABAAreceptor antagonist bicuculline was applied at 10 lmol/L, IIDs and PIDs disappeared, and IDs remained at decreased intervals. These results indicated that there are dynamic changes in the hippocampal network preceding the onset of IDs, and GABAAreceptor activity suppresses the transition from IIDs to IDs in juvenile mouse hippocampus.
文摘Cu films were electrodeposited under a magnetic field of 5 T on the Pt disc electrodes with diameters of 3 mm ~ 25 μm.Such magnetoelectrodeposited(MED) Cu films were employed as an electrode,and cyclic voltammograms were measured for the electrochemical reactions of an amino acid of alanine.Chiral behavior was observed as oxidation current difference between the enantiomers.Clear magnetoelectrochemical chirality was observed on the micro-disk electrode and on the kinetic-controlled electrode process of alanine.The chiral response was durable for more than 10 cycles of the consecutive voltammetric measurements of the enantiomers.
基金supported by the National Natural Science Foundation of China(No.61076118)
文摘Recent work in the field ofneurophysiology has demonstrated that, by observing the firing characteristic of action potentials （AP） and the exchange pattern of signals between neurons, it is possible to reveal the nature of ＂memory＂ and ＂thinking＂ and help humans to understand how the brain works. To address these needs, we developed a prototype fully integrated circuit （IC） with micro-electrode array （MEA） for neural recording. In this scheme, the microelectrode array is composed by 64 detection electrodes and 2 reference electrodes. The proposed IC consists of 8 recording channels with an area of 5 x 5 mm2. Each channel can operate independently to process the neural signal by amplifying, filtering, etc. The chip is fabricated in 0.5-#m CMOS technology. The simulated and measured results show the system provides an effective device for recording feeble signal such as neural signals.
