背景:微小RNA参与调控干细胞增殖、分化、衰老等过程,通过了解Let-7家族成员Let-7c对骨髓间充质干细胞向神经细胞分化的影响可以为干细胞移植治疗提供新思路。目的:探讨Let-7c慢病毒载体在体外诱导大鼠骨髓间充质干细胞向神经细胞分化...背景:微小RNA参与调控干细胞增殖、分化、衰老等过程,通过了解Let-7家族成员Let-7c对骨髓间充质干细胞向神经细胞分化的影响可以为干细胞移植治疗提供新思路。目的:探讨Let-7c慢病毒载体在体外诱导大鼠骨髓间充质干细胞向神经细胞分化中的作用。方法:构建Let-7c上调及下调慢病毒载体并体外转染大鼠骨髓间充质干细胞,筛选最适转染复数;实验分为未转染组、阴性转染组(转染空白慢病毒)、转染上调组(转染LV-rno-Let-7c-up)、转染下调组(转染LV-rno-Let-7c-5p-inhibition);采用法舒地尔诱导转染后的大鼠骨髓间充质干细胞分化为神经细胞,倒置荧光显微镜下观察骨髓间充质干细胞转染后荧光表达,免疫细胞化学染色检测神经元标记物NSE和MAP-2的表达,RT-PCR检测MAP-2 m RNA的表达,MTT法检测细胞存活率。结果与结论:倒置荧光显微镜下观察大鼠LV-rno-Let-7c-up和LV-rno-Let-7c-5p-inhibition慢病毒载体转染成功。法舒地尔可以诱导骨髓间充质干细胞分化为神经细胞,与阴性转染组相比,转染上调组的诱导效率升高(P<0.05),NSE和MAP-2表达率上升(P<0.05),转染下调组的诱导效率下降,NSE和MAP-2表达率下降(P<0.05)。转染LV-rno-Let-7c-up后骨髓间充质干细胞经法舒地尔诱导向神经细胞分化比率增加,Let-7c可能具有促进骨髓间充质干细胞向神经细胞分化的作用。展开更多
Retinoic acid can cause many types of cells,including mouse neuroblastoma Neuro-2 A cells,to differentiate into neurons.However,it is still unknown whether microRNAs(miRNAs)play a role in this neuronal differentiation...Retinoic acid can cause many types of cells,including mouse neuroblastoma Neuro-2 A cells,to differentiate into neurons.However,it is still unknown whether microRNAs(miRNAs)play a role in this neuronal differentiation.To address this issue,real-time polymerase chain reaction assays were used to detect the expression of several differentiation-related miRNAs during the differentiation of retinoic acid-treated Neuro-2 A cells.The results revealed that miR-124 and miR-9 were upregulated,while miR-125 b was downregulated in retinoic acid-treated Neuro-2 A cells.To identify the miRNA that may play a key role,miR-124 expression was regulated by transfection of miRNA mimics or inhibitors.Morphological analysis results showed that inhibition of miR-124 expression reversed the effects of retinoic acid on neurite outgrowth.Moreover,miR-124 overexpression alone caused Neuro-2 A cells to differentiate into neurons,and its inhibitor could block this effect.These results suggest that miR-124 plays an important role in retinoic acid-induced differentiation of Neuro-2 A cells.展开更多
Submarine micro-geomorphology is a geo-morphological type occurring in shallow and surface areas of seabed.The combined relationships and distribution of the micro-geomorphology indirectly reflect coupling relationshi...Submarine micro-geomorphology is a geo-morphological type occurring in shallow and surface areas of seabed.The combined relationships and distribution of the micro-geomorphology indirectly reflect coupling relationships among the sediment deposition,dynamic environment,and geomorphologic evolution.Spatial differentiation and dynamic changes in micro-geomorphology were studied based on acoustic data interpretation from a wide range(3200 km^(2))of the Huanghe(Yellow)River delta(HRD).The combination of the sub-bottom profiler and the side-scan sonar methods allowed for the identification of submarine shallow micro-geomorphologic types,as well as their scale and spatial distributions.There were seven typical micro-geomorphologic features in the shallow and surface areas of the HRD,including buried ancient channels,stratigraphic disturbances,scour troughs,sand waves,pits,erosional remnants,and sand spots.The coupling and superposition of the sediment,sediment characteristics,seabed scouring and silting,and hydrodynamic conditions of the Huanghe River had combined effects on the patterns of micro-geomorphologic types,characteristics,and ranges.From the perspective of acoustic profile interpretations,the scale,range,and spatial locations of the microgeomorphology in the HRD revealed seasonal variation characteristics,and the spatial distributions displayed significant regional differentiation characteristics.In addition,strong stratigraphic disturbances and areas with densely distributed buried ancient channels reflected the activity and instability of the submarine shallow strata.Through the interpretation of the sub-bottom profile detection data,the diversion processes of the flow paths in the lower reaches of the Huanghe River were obtained for a certain historical period in the coastal waters of the HRD.This study further clarified the relationships between the micro-geomorphologic features and spatial combinations,which is important for research on micro-geomorphologic features and their dynamic mechanisms.展开更多
文摘背景:微小RNA参与调控干细胞增殖、分化、衰老等过程,通过了解Let-7家族成员Let-7c对骨髓间充质干细胞向神经细胞分化的影响可以为干细胞移植治疗提供新思路。目的:探讨Let-7c慢病毒载体在体外诱导大鼠骨髓间充质干细胞向神经细胞分化中的作用。方法:构建Let-7c上调及下调慢病毒载体并体外转染大鼠骨髓间充质干细胞,筛选最适转染复数;实验分为未转染组、阴性转染组(转染空白慢病毒)、转染上调组(转染LV-rno-Let-7c-up)、转染下调组(转染LV-rno-Let-7c-5p-inhibition);采用法舒地尔诱导转染后的大鼠骨髓间充质干细胞分化为神经细胞,倒置荧光显微镜下观察骨髓间充质干细胞转染后荧光表达,免疫细胞化学染色检测神经元标记物NSE和MAP-2的表达,RT-PCR检测MAP-2 m RNA的表达,MTT法检测细胞存活率。结果与结论:倒置荧光显微镜下观察大鼠LV-rno-Let-7c-up和LV-rno-Let-7c-5p-inhibition慢病毒载体转染成功。法舒地尔可以诱导骨髓间充质干细胞分化为神经细胞,与阴性转染组相比,转染上调组的诱导效率升高(P<0.05),NSE和MAP-2表达率上升(P<0.05),转染下调组的诱导效率下降,NSE和MAP-2表达率下降(P<0.05)。转染LV-rno-Let-7c-up后骨髓间充质干细胞经法舒地尔诱导向神经细胞分化比率增加,Let-7c可能具有促进骨髓间充质干细胞向神经细胞分化的作用。
基金supported by the Natural Science Foundation of Shanghai of China,No.16ZR1410500(to SZD)
文摘Retinoic acid can cause many types of cells,including mouse neuroblastoma Neuro-2 A cells,to differentiate into neurons.However,it is still unknown whether microRNAs(miRNAs)play a role in this neuronal differentiation.To address this issue,real-time polymerase chain reaction assays were used to detect the expression of several differentiation-related miRNAs during the differentiation of retinoic acid-treated Neuro-2 A cells.The results revealed that miR-124 and miR-9 were upregulated,while miR-125 b was downregulated in retinoic acid-treated Neuro-2 A cells.To identify the miRNA that may play a key role,miR-124 expression was regulated by transfection of miRNA mimics or inhibitors.Morphological analysis results showed that inhibition of miR-124 expression reversed the effects of retinoic acid on neurite outgrowth.Moreover,miR-124 overexpression alone caused Neuro-2 A cells to differentiate into neurons,and its inhibitor could block this effect.These results suggest that miR-124 plays an important role in retinoic acid-induced differentiation of Neuro-2 A cells.
基金Supported by the General Program of Natural Science Foundation of Shandong Province(No.ZR2020MD063)the Youth Program of Natural Science Foundation of Shandong Province(No.ZR2013DQ025)the National Natural Science Foundation of China and Shandong Province Joint Funds(No.U1706214)。
文摘Submarine micro-geomorphology is a geo-morphological type occurring in shallow and surface areas of seabed.The combined relationships and distribution of the micro-geomorphology indirectly reflect coupling relationships among the sediment deposition,dynamic environment,and geomorphologic evolution.Spatial differentiation and dynamic changes in micro-geomorphology were studied based on acoustic data interpretation from a wide range(3200 km^(2))of the Huanghe(Yellow)River delta(HRD).The combination of the sub-bottom profiler and the side-scan sonar methods allowed for the identification of submarine shallow micro-geomorphologic types,as well as their scale and spatial distributions.There were seven typical micro-geomorphologic features in the shallow and surface areas of the HRD,including buried ancient channels,stratigraphic disturbances,scour troughs,sand waves,pits,erosional remnants,and sand spots.The coupling and superposition of the sediment,sediment characteristics,seabed scouring and silting,and hydrodynamic conditions of the Huanghe River had combined effects on the patterns of micro-geomorphologic types,characteristics,and ranges.From the perspective of acoustic profile interpretations,the scale,range,and spatial locations of the microgeomorphology in the HRD revealed seasonal variation characteristics,and the spatial distributions displayed significant regional differentiation characteristics.In addition,strong stratigraphic disturbances and areas with densely distributed buried ancient channels reflected the activity and instability of the submarine shallow strata.Through the interpretation of the sub-bottom profile detection data,the diversion processes of the flow paths in the lower reaches of the Huanghe River were obtained for a certain historical period in the coastal waters of the HRD.This study further clarified the relationships between the micro-geomorphologic features and spatial combinations,which is important for research on micro-geomorphologic features and their dynamic mechanisms.
