A novel dammarane-type triterpene oligoglycoside, named ginsenoside-Rg6 3, was isolated from the stem-leaves of Panax ginseng C. A. Mey., together with two known ones, 20(S)-ginsenoside-Rg2 1 and 20(R)-ginsenoside-Rg2...A novel dammarane-type triterpene oligoglycoside, named ginsenoside-Rg6 3, was isolated from the stem-leaves of Panax ginseng C. A. Mey., together with two known ones, 20(S)-ginsenoside-Rg2 1 and 20(R)-ginsenoside-Rg2 2. On the basis of chemical and physicochemical evidence , the structure of ginsenoside-Rg6 have been elucidated as 6-O-(-L-rhamnosyl-(1?2)-(-D-glucopyranosyl-dammarane-(E)-20(22), 24-diene-3(, 6(, 12(-triol.展开更多
全球气候变暖和大气CO_(2)浓度(Ca)急剧上升已成为不争的事实,并对森林生态系统有着深远的影响,全面理解森林生态系统对全球变化的响应至关重要。中亚干旱区特有树种雪岭云杉(Picea schrenkiana Fisch. et Mey)树轮稳定碳同位素(δ^(13...全球气候变暖和大气CO_(2)浓度(Ca)急剧上升已成为不争的事实,并对森林生态系统有着深远的影响,全面理解森林生态系统对全球变化的响应至关重要。中亚干旱区特有树种雪岭云杉(Picea schrenkiana Fisch. et Mey)树轮稳定碳同位素(δ^(13)C)和水分利用效率(iWUE)对气候变暖和大气CO2浓度急剧上升的响应缺乏研究。本研究利用天山西部伊犁河流域的雪岭云杉树轮样本,使用树木年代学方法和树轮稳定碳同位素技术,建立树轮宽度年表、稳定碳同位素(δ^(13)C)以及内禀水分利用效率(iWUE)序列,分析了树轮δ13C和iWUE的长期变化特征,探讨了树轮δ13C和iWUE对气候的响应规律以及iWUE与树轮宽度的关系。结果表明,主要的气候因子并没有强烈地限制树木径向生长;夏季平均气温对树轮δ^(13)C分馏有重要影响,iWUE的长期变化受到全球Ca增加和升温趋势影响,但直接影响iWUE年际变化的主要因素是饱和水汽压亏缺(VPD)。全球升温和Ca增加导致了iWUE的持续升高,但并没有导致雪岭云杉树木径向生长的明显增加。展开更多
A water-soluble pectin SB_~1-1 was isolated and purified from the root of Panax ginseng C. A. Mey. The HPLC analysis indicates that SB_~1-1 is homogenous. Its molecular weight was estimated via gel filtration to be 10...A water-soluble pectin SB_~1-1 was isolated and purified from the root of Panax ginseng C. A. Mey. The HPLC analysis indicates that SB_~1-1 is homogenous. Its molecular weight was estimated via gel filtration to be 10000. The GC analysis indicated that it contains the monosaccharides of GalA, Gal, Ara and Rha. Their molar ratio is 2.10∶1.00∶0.12∶0.13. Partial hydrolysis with acid, pectinase treatment, periodate oxidation, Smith degradation, methylation analyses, GC/MS analyses and NMR analyses were used for the structure analyses of SB_~1-1 . The results reveal that SB_~1-1 has a lower branched structure. The main chain is composed of GalA and Gal; the inner part is α-1,4-linked-GalA; the border is 1,4-linked-Gal. Some of the 1,4-linked-GalA and 1,4-linked-Gal residues are substituted at O6. On an average, there is one branch for every ten hexose residues. The side chain is composed of 1,6-linked-Gal and 1,3,6-linked-Gal. The nonreduced end is composed of Rha, Ara and Gal. The main glycosidic link of SB_~1-1 has an α configuration.展开更多
A new coumarin was obtained from the stems and roots of Wikstroemia indica (L.) C. A. Mey. Its structure was elucidated as methyl 3-(2-hydroxy-4-(7-hydroxy-6-methoxy-2-oxo-2H-chromen-3-yloxy) phenyl) propanoate ...A new coumarin was obtained from the stems and roots of Wikstroemia indica (L.) C. A. Mey. Its structure was elucidated as methyl 3-(2-hydroxy-4-(7-hydroxy-6-methoxy-2-oxo-2H-chromen-3-yloxy) phenyl) propanoate by spectroscopic methods.展开更多
[ Objective ] The aim was to select appropriate sterilization methods and explants for tissue culture of Haloxylon ammodendron ( C. A. Mey. ). [Method] H. ammodendron (C. A. Mey. ) seeds were used as experimental ...[ Objective ] The aim was to select appropriate sterilization methods and explants for tissue culture of Haloxylon ammodendron ( C. A. Mey. ). [Method] H. ammodendron (C. A. Mey. ) seeds were used as experimental materials to investigate the sterilization results of different sterilization treatments and the effect of different seed sizes on the survival rate of aseptic seedlings. [ Result] Sterilization of H. ammodendron ( C.A. Mey. ) seeds achieved the best result by using 75% alcohol for 15 min and 0.1% mercuric chloride for 8 min. Seeds with diameter φ 〉 2.0 mm were used as explants and achieved relatively high surviv- al rate of aseptic seedlings, which reached above 65%. [ Conclusion] This study established a surface sterilization method for tissue culture of H. ammodendron ( C. A. Mey. ) Bunge seeds.展开更多
The clonal diversity of Clintonia udensis Trautv.et Mey.was detected by ISSR markers among 16 populations,and its correlation with ecological factors was analyzed as well in this work.Results showed that individuals(c...The clonal diversity of Clintonia udensis Trautv.et Mey.was detected by ISSR markers among 16 populations,and its correlation with ecological factors was analyzed as well in this work.Results showed that individuals(clonal ramets)per genotype were 1.12 and 1.149 at population and species levels,respectively,and that the 16 populations were all multiclonal.The detected genotypes were localized,without exception,within populations but demonstrated relatively high clonal differentiation among populations.The clonal diversity of the studied populations was high,with the average Simpson's index of 0.975,while the genets showed a clonal architecture of"guerilla".The population genetic diversities revealed by genet were consistent with those by ramet,further confirming their genetic differentiation among populations.And its genotype diversity within populations probably resulted largely from the frequent seedling regeneration and self-compatibility.In addition,the correlation analysis further revealed that,among the ecological factors,Simpson's index of C.udensis had a significant positive correlation(P<0.05)with pH values in the soil but not others.展开更多
A high performance liquid chromatography(HPLC) with UV detection was established for simultaneous determination of saponins in the leaf of Panax ginseng C. A. Mey. Nine ginsenosides(Rbl, Rb2, Rb3, Rc, Rd, F1, F2, F...A high performance liquid chromatography(HPLC) with UV detection was established for simultaneous determination of saponins in the leaf of Panax ginseng C. A. Mey. Nine ginsenosides(Rbl, Rb2, Rb3, Rc, Rd, F1, F2, F3, F5) and notoginsenoside Fe(NFe) were studied. Among the saponins, the ginsenosides F1, F2, F3, F5 and NFe were determined by HPLC-UV method for the first time. The determination of the ginsenosides via the HPLC-UV method was performed on a reversed-phase C18 column with gradient elution in 40 min. The linearity, precision, accuracy, and detection limit for determining the saponins were studied and the samples from different areas in China were analyzed. The HPLC-ESI-MS was used to identify the saponins. The results indicate that the HPLC-UV provided a good accuracy, reproducibility and sensitivity for the determination of the ten saponins.展开更多
Panax japonicus and its approximation varieties,such as Rhizoma Panacis Majoris and Panax japonicus C. A. Mey. var.major (Burk.) C.Y. Wu et K.M. Feng belong to Panax,which are less commonly used traditional Chinese ...Panax japonicus and its approximation varieties,such as Rhizoma Panacis Majoris and Panax japonicus C. A. Mey. var.major (Burk.) C.Y. Wu et K.M. Feng belong to Panax,which are less commonly used traditional Chinese medicine. Because of similar traits and effectiveness,they were always used as one type of medicine for a long time. Aiming at this phenomenon,the chemical composition and contents of P. japonicus and its approximation varieties from different area were compared in order to provide a chemical basis for clarifying the classification of the genus.展开更多
The macrosporogenesis and microsporogenesis, the formation of female and male gametophyte, the process of double fertilization, the development of embryo andendosperm, and the formation of seeds of ginseng (Panax gins...The macrosporogenesis and microsporogenesis, the formation of female and male gametophyte, the process of double fertilization, the development of embryo andendosperm, and the formation of seeds of ginseng (Panax ginseng C. A. Mey.)展开更多
基金The Ninth 5-year Plan" Key Science and Technique R & D Programme Foundation of China (96-901-01-12A).
文摘A novel dammarane-type triterpene oligoglycoside, named ginsenoside-Rg6 3, was isolated from the stem-leaves of Panax ginseng C. A. Mey., together with two known ones, 20(S)-ginsenoside-Rg2 1 and 20(R)-ginsenoside-Rg2 2. On the basis of chemical and physicochemical evidence , the structure of ginsenoside-Rg6 have been elucidated as 6-O-(-L-rhamnosyl-(1?2)-(-D-glucopyranosyl-dammarane-(E)-20(22), 24-diene-3(, 6(, 12(-triol.
文摘全球气候变暖和大气CO_(2)浓度(Ca)急剧上升已成为不争的事实,并对森林生态系统有着深远的影响,全面理解森林生态系统对全球变化的响应至关重要。中亚干旱区特有树种雪岭云杉(Picea schrenkiana Fisch. et Mey)树轮稳定碳同位素(δ^(13)C)和水分利用效率(iWUE)对气候变暖和大气CO2浓度急剧上升的响应缺乏研究。本研究利用天山西部伊犁河流域的雪岭云杉树轮样本,使用树木年代学方法和树轮稳定碳同位素技术,建立树轮宽度年表、稳定碳同位素(δ^(13)C)以及内禀水分利用效率(iWUE)序列,分析了树轮δ13C和iWUE的长期变化特征,探讨了树轮δ13C和iWUE对气候的响应规律以及iWUE与树轮宽度的关系。结果表明,主要的气候因子并没有强烈地限制树木径向生长;夏季平均气温对树轮δ^(13)C分馏有重要影响,iWUE的长期变化受到全球Ca增加和升温趋势影响,但直接影响iWUE年际变化的主要因素是饱和水汽压亏缺(VPD)。全球升温和Ca增加导致了iWUE的持续升高,但并没有导致雪岭云杉树木径向生长的明显增加。
文摘A water-soluble pectin SB_~1-1 was isolated and purified from the root of Panax ginseng C. A. Mey. The HPLC analysis indicates that SB_~1-1 is homogenous. Its molecular weight was estimated via gel filtration to be 10000. The GC analysis indicated that it contains the monosaccharides of GalA, Gal, Ara and Rha. Their molar ratio is 2.10∶1.00∶0.12∶0.13. Partial hydrolysis with acid, pectinase treatment, periodate oxidation, Smith degradation, methylation analyses, GC/MS analyses and NMR analyses were used for the structure analyses of SB_~1-1 . The results reveal that SB_~1-1 has a lower branched structure. The main chain is composed of GalA and Gal; the inner part is α-1,4-linked-GalA; the border is 1,4-linked-Gal. Some of the 1,4-linked-GalA and 1,4-linked-Gal residues are substituted at O6. On an average, there is one branch for every ten hexose residues. The side chain is composed of 1,6-linked-Gal and 1,3,6-linked-Gal. The nonreduced end is composed of Rha, Ara and Gal. The main glycosidic link of SB_~1-1 has an α configuration.
文摘A new coumarin was obtained from the stems and roots of Wikstroemia indica (L.) C. A. Mey. Its structure was elucidated as methyl 3-(2-hydroxy-4-(7-hydroxy-6-methoxy-2-oxo-2H-chromen-3-yloxy) phenyl) propanoate by spectroscopic methods.
基金Supported by Open Project of Key Laboratory of Tarim Animal Husbandry Science and Technology from Xinjiang Production & Construction Crops(HS20901)
文摘[ Objective ] The aim was to select appropriate sterilization methods and explants for tissue culture of Haloxylon ammodendron ( C. A. Mey. ). [Method] H. ammodendron (C. A. Mey. ) seeds were used as experimental materials to investigate the sterilization results of different sterilization treatments and the effect of different seed sizes on the survival rate of aseptic seedlings. [ Result] Sterilization of H. ammodendron ( C.A. Mey. ) seeds achieved the best result by using 75% alcohol for 15 min and 0.1% mercuric chloride for 8 min. Seeds with diameter φ 〉 2.0 mm were used as explants and achieved relatively high surviv- al rate of aseptic seedlings, which reached above 65%. [ Conclusion] This study established a surface sterilization method for tissue culture of H. ammodendron ( C. A. Mey. ) Bunge seeds.
基金the National Natural Science Foundation of China(Grant No.30670129)Doctoral Fund of the Ministry of Education of China(Grant No.20040697010)Natural Science Foundation of Shaanxi Province(Grant No.2005C117)
文摘The clonal diversity of Clintonia udensis Trautv.et Mey.was detected by ISSR markers among 16 populations,and its correlation with ecological factors was analyzed as well in this work.Results showed that individuals(clonal ramets)per genotype were 1.12 and 1.149 at population and species levels,respectively,and that the 16 populations were all multiclonal.The detected genotypes were localized,without exception,within populations but demonstrated relatively high clonal differentiation among populations.The clonal diversity of the studied populations was high,with the average Simpson's index of 0.975,while the genets showed a clonal architecture of"guerilla".The population genetic diversities revealed by genet were consistent with those by ramet,further confirming their genetic differentiation among populations.And its genotype diversity within populations probably resulted largely from the frequent seedling regeneration and self-compatibility.In addition,the correlation analysis further revealed that,among the ecological factors,Simpson's index of C.udensis had a significant positive correlation(P<0.05)with pH values in the soil but not others.
文摘A high performance liquid chromatography(HPLC) with UV detection was established for simultaneous determination of saponins in the leaf of Panax ginseng C. A. Mey. Nine ginsenosides(Rbl, Rb2, Rb3, Rc, Rd, F1, F2, F3, F5) and notoginsenoside Fe(NFe) were studied. Among the saponins, the ginsenosides F1, F2, F3, F5 and NFe were determined by HPLC-UV method for the first time. The determination of the ginsenosides via the HPLC-UV method was performed on a reversed-phase C18 column with gradient elution in 40 min. The linearity, precision, accuracy, and detection limit for determining the saponins were studied and the samples from different areas in China were analyzed. The HPLC-ESI-MS was used to identify the saponins. The results indicate that the HPLC-UV provided a good accuracy, reproducibility and sensitivity for the determination of the ten saponins.
基金Supported by the National Natural Foundation of China(30873383)~~
文摘Panax japonicus and its approximation varieties,such as Rhizoma Panacis Majoris and Panax japonicus C. A. Mey. var.major (Burk.) C.Y. Wu et K.M. Feng belong to Panax,which are less commonly used traditional Chinese medicine. Because of similar traits and effectiveness,they were always used as one type of medicine for a long time. Aiming at this phenomenon,the chemical composition and contents of P. japonicus and its approximation varieties from different area were compared in order to provide a chemical basis for clarifying the classification of the genus.
文摘The macrosporogenesis and microsporogenesis, the formation of female and male gametophyte, the process of double fertilization, the development of embryo andendosperm, and the formation of seeds of ginseng (Panax ginseng C. A. Mey.)