Previous studies have found that methylmercury can damage hippocampal neurons and accord- ingly cause cognitive dysfunction. However, a non-invasive, safe and accurate detection method for detecting hippocampal injury...Previous studies have found that methylmercury can damage hippocampal neurons and accord- ingly cause cognitive dysfunction. However, a non-invasive, safe and accurate detection method for detecting hippocampal injury has yet to be developed. This study aimed to detect methylmercury-induced damage on hippocampal tissue using proton magnetic resonance spectroscopy. Rats were given a subcutaneous injection of 4 and 2 mg/kg methylmercury into the neck for 50 consecutive days. Water maze and pathology tests confirmed that cognitive function had been impaired and that the ultrastructure of hippocampal tissue was altered after injection. The results of proton magnetic resonance spectroscopy revealed that the nitrogen-acetyl aspartate/ creatine, choline complex/creatine and myoinositol/creatine ratio in rat hippocampal tissue were unchanged. Therefore, proton magnetic resonance spectroscopy can not be used to determine structural damage in the adult rat hippocampus caused by methylmercury chloride.展开更多
目的:通过观察氯化甲基汞(MMC)诱导大鼠不同发育阶段脑神经细胞凋亡,揭示甲基汞致脑发育损伤的分子机制。方法:建立长期接触低剂量氯化甲基汞致脑发育损伤大鼠动物实验模型。采用荧光标记DNA片段分析试剂盒ApoAlertTM DNA Fragmentation...目的:通过观察氯化甲基汞(MMC)诱导大鼠不同发育阶段脑神经细胞凋亡,揭示甲基汞致脑发育损伤的分子机制。方法:建立长期接触低剂量氯化甲基汞致脑发育损伤大鼠动物实验模型。采用荧光标记DNA片段分析试剂盒ApoAlertTM DNA Fragmentation Assay Kit检测长期接触低剂量氯化甲基汞对不同发育阶段仔鼠不同脑区(大脑、小脑和海马)神经元凋亡的影响。结果:实验组和对照组出生后不同时间点仔鼠脑神经元都存在凋亡。随仔鼠脑汞含量的增加,大脑、小脑和海马神经元凋亡均明显增加(P<0.05)。结论:长期接触低剂量氯化甲基汞致脑发育损伤与其诱导神经元过渡凋亡有关。展开更多
基金supported by the National Natural Science Foundation of China,No81060231,81160338Hubei Province Natural Science Foundation of China,No.2013CFB277
文摘Previous studies have found that methylmercury can damage hippocampal neurons and accord- ingly cause cognitive dysfunction. However, a non-invasive, safe and accurate detection method for detecting hippocampal injury has yet to be developed. This study aimed to detect methylmercury-induced damage on hippocampal tissue using proton magnetic resonance spectroscopy. Rats were given a subcutaneous injection of 4 and 2 mg/kg methylmercury into the neck for 50 consecutive days. Water maze and pathology tests confirmed that cognitive function had been impaired and that the ultrastructure of hippocampal tissue was altered after injection. The results of proton magnetic resonance spectroscopy revealed that the nitrogen-acetyl aspartate/ creatine, choline complex/creatine and myoinositol/creatine ratio in rat hippocampal tissue were unchanged. Therefore, proton magnetic resonance spectroscopy can not be used to determine structural damage in the adult rat hippocampus caused by methylmercury chloride.
文摘目的:通过观察氯化甲基汞(MMC)诱导大鼠不同发育阶段脑神经细胞凋亡,揭示甲基汞致脑发育损伤的分子机制。方法:建立长期接触低剂量氯化甲基汞致脑发育损伤大鼠动物实验模型。采用荧光标记DNA片段分析试剂盒ApoAlertTM DNA Fragmentation Assay Kit检测长期接触低剂量氯化甲基汞对不同发育阶段仔鼠不同脑区(大脑、小脑和海马)神经元凋亡的影响。结果:实验组和对照组出生后不同时间点仔鼠脑神经元都存在凋亡。随仔鼠脑汞含量的增加,大脑、小脑和海马神经元凋亡均明显增加(P<0.05)。结论:长期接触低剂量氯化甲基汞致脑发育损伤与其诱导神经元过渡凋亡有关。
