<strong>Background:</strong> The aim of this study was to evaluate vision related quality of life (VRQoL) in patients with retinitis pigmentosa (RP) after suprachoroidal umbilical cord derived mesenchymal ...<strong>Background:</strong> The aim of this study was to evaluate vision related quality of life (VRQoL) in patients with retinitis pigmentosa (RP) after suprachoroidal umbilical cord derived mesenchymal stem cell (UC-MSC) treatment. <strong>Methods:</strong> The patients were evaluated regarding to the VRQoL before the treatment and at the end of the first year. The study was performed in an affiliated hospital of a university between 2018 and 2020. The patients were operated by a single surgeon and evaluated at baseline and at first, sixth and twelfth month after stem cell implantation. To assess patients’ subjective visual situation, we used Impact of Vision Impairment (IVI) Profile 28-item questionnaire. It basically evaluated vision-related activities in three subscales: “reading and accessing information”, “mobility and independence” and “emotional well-being”. This test is established to assess VRQoL in low vision patients. <strong>Results:</strong> A total of 123 people, ranging in age from 18 to 48 years, participated in the study and 43.9% (n = 54) were women. The study patients were followed up for one year and the questionnaire was filled by patients at baseline and one year after surgery. In all groups, no difficulty was observed in understanding the questionnaire. The results showed significant improvements in VRQoL after stem cell treatment (p < 0.05). The analysis of each subscale score including “reading and accessing information”, “mobility and independence” and “emotional well-being” before and after treatment showed significant improvements in all subscale scores (p < 0.05). <strong>Conclusions:</strong> IVI 28 item questionnaire seems to be an effective test for the assessment of VRQoL in low vision patients. The suprachoroidal implantation of UC-MSC for the treatment of RP can improve the quality of life of these patients.展开更多
目的:观察姜黄素联合骨髓间充质干细胞(marrow mesenchymal stem cell,MSCs)对大鼠溃疡性结肠炎(ulcerative colitis,UC)的疗效,并探讨辅助性T细胞17(T help cell 17,Th17)及调节性T细胞(regulatory T cell,Treg)平衡在其中的作用机制....目的:观察姜黄素联合骨髓间充质干细胞(marrow mesenchymal stem cell,MSCs)对大鼠溃疡性结肠炎(ulcerative colitis,UC)的疗效,并探讨辅助性T细胞17(T help cell 17,Th17)及调节性T细胞(regulatory T cell,Treg)平衡在其中的作用机制.方法:采用三硝基苯磺酸(trinitro-benzenesulfonic acid,TNBS)灌肠制备大鼠UC模型,将动物分为5组,分别为正常对照组(A组)、模型组(B组),MSCs组(C组),姜黄素组(D组),姜黄素联合MSCs组(E组).观察各组大鼠的行为、粪便性状及血便情况,计算结肠大体形态评分(gross morphological score,GMS)及病理损伤评分(histopathologic damage score,HDS),并通过流式细胞术(flow cytometer,FCM)检测各组Th17及Treg占CD4+T细胞的数量百分比;免疫组织化学(immunohistochemistry,IHC)检测白介素-17、Foxp3在结肠组织的表达.结果:(1)与正常组比较,模型组大鼠GMS、HDS均明显升高(0.00±0.00 vs 4.63±0.74;0.25±0.46 vs 4.00±0.53,P<0.01),Treg细胞在外周血及结肠组织的表达均下调,Th17的表达均上调,差异均有统计学意义(8.01±0.29 vs1.16±0.18;9.50±1.60 vs 0.88±0.64;0.63±0.13 vs 4.56±0.21;0.88±0.64 vs 7.75±1.16,P<0.01);(2)与模型组相比,各治疗组GMS、HDS均明显降低,差异有统计学意义(4.63±0.74 vs 2.12±0.64,2.00±0.53,1.00±0.53;4.00±0.53 vs 2.00±0.53,1.88±0.83,1.25±0.46,P<0.01);各治疗组Treg细胞表达均明显上调,而Th17细胞的表达均明显下调,差异有统计学意义(1.16±0.18 vs 3.29±0.18,3.19±0.20,5.00±0.19;0.88±0.64 vs 3.75±0.46,4.00±0.92,6.88±1.25;4.56±0.21 vs 2.60±0.15,2.59±0.23,1.52±0.21;0.88±0.64 vs3.75±0.46,4.00±0.92,6.88±1.25,P<0.01);(3)联合治疗组GMS、HDS较单药物治疗组降低(1.00±0.53 vs 2.12±0.64,2.00±0.53;1.25±0.46 vs 2.00±0.53,1.88±0.83,P<0.05),且Treg细胞表达均显著上调,而Th17细胞表达均显著下调,差异有统计学意义(5.00±0.19vs 3.29±0.18,3.19±0.20;6.88±1.25 vs 3.75±0.46,4.00±0.92;1.52±0.21 vs 2.60±0.15,2.59±0.23;2.38±0.92 vs 5.00展开更多
文摘<strong>Background:</strong> The aim of this study was to evaluate vision related quality of life (VRQoL) in patients with retinitis pigmentosa (RP) after suprachoroidal umbilical cord derived mesenchymal stem cell (UC-MSC) treatment. <strong>Methods:</strong> The patients were evaluated regarding to the VRQoL before the treatment and at the end of the first year. The study was performed in an affiliated hospital of a university between 2018 and 2020. The patients were operated by a single surgeon and evaluated at baseline and at first, sixth and twelfth month after stem cell implantation. To assess patients’ subjective visual situation, we used Impact of Vision Impairment (IVI) Profile 28-item questionnaire. It basically evaluated vision-related activities in three subscales: “reading and accessing information”, “mobility and independence” and “emotional well-being”. This test is established to assess VRQoL in low vision patients. <strong>Results:</strong> A total of 123 people, ranging in age from 18 to 48 years, participated in the study and 43.9% (n = 54) were women. The study patients were followed up for one year and the questionnaire was filled by patients at baseline and one year after surgery. In all groups, no difficulty was observed in understanding the questionnaire. The results showed significant improvements in VRQoL after stem cell treatment (p < 0.05). The analysis of each subscale score including “reading and accessing information”, “mobility and independence” and “emotional well-being” before and after treatment showed significant improvements in all subscale scores (p < 0.05). <strong>Conclusions:</strong> IVI 28 item questionnaire seems to be an effective test for the assessment of VRQoL in low vision patients. The suprachoroidal implantation of UC-MSC for the treatment of RP can improve the quality of life of these patients.
文摘目的:观察姜黄素联合骨髓间充质干细胞(marrow mesenchymal stem cell,MSCs)对大鼠溃疡性结肠炎(ulcerative colitis,UC)的疗效,并探讨辅助性T细胞17(T help cell 17,Th17)及调节性T细胞(regulatory T cell,Treg)平衡在其中的作用机制.方法:采用三硝基苯磺酸(trinitro-benzenesulfonic acid,TNBS)灌肠制备大鼠UC模型,将动物分为5组,分别为正常对照组(A组)、模型组(B组),MSCs组(C组),姜黄素组(D组),姜黄素联合MSCs组(E组).观察各组大鼠的行为、粪便性状及血便情况,计算结肠大体形态评分(gross morphological score,GMS)及病理损伤评分(histopathologic damage score,HDS),并通过流式细胞术(flow cytometer,FCM)检测各组Th17及Treg占CD4+T细胞的数量百分比;免疫组织化学(immunohistochemistry,IHC)检测白介素-17、Foxp3在结肠组织的表达.结果:(1)与正常组比较,模型组大鼠GMS、HDS均明显升高(0.00±0.00 vs 4.63±0.74;0.25±0.46 vs 4.00±0.53,P<0.01),Treg细胞在外周血及结肠组织的表达均下调,Th17的表达均上调,差异均有统计学意义(8.01±0.29 vs1.16±0.18;9.50±1.60 vs 0.88±0.64;0.63±0.13 vs 4.56±0.21;0.88±0.64 vs 7.75±1.16,P<0.01);(2)与模型组相比,各治疗组GMS、HDS均明显降低,差异有统计学意义(4.63±0.74 vs 2.12±0.64,2.00±0.53,1.00±0.53;4.00±0.53 vs 2.00±0.53,1.88±0.83,1.25±0.46,P<0.01);各治疗组Treg细胞表达均明显上调,而Th17细胞的表达均明显下调,差异有统计学意义(1.16±0.18 vs 3.29±0.18,3.19±0.20,5.00±0.19;0.88±0.64 vs 3.75±0.46,4.00±0.92,6.88±1.25;4.56±0.21 vs 2.60±0.15,2.59±0.23,1.52±0.21;0.88±0.64 vs3.75±0.46,4.00±0.92,6.88±1.25,P<0.01);(3)联合治疗组GMS、HDS较单药物治疗组降低(1.00±0.53 vs 2.12±0.64,2.00±0.53;1.25±0.46 vs 2.00±0.53,1.88±0.83,P<0.05),且Treg细胞表达均显著上调,而Th17细胞表达均显著下调,差异有统计学意义(5.00±0.19vs 3.29±0.18,3.19±0.20;6.88±1.25 vs 3.75±0.46,4.00±0.92;1.52±0.21 vs 2.60±0.15,2.59±0.23;2.38±0.92 vs 5.00
