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西班牙根结线虫rDNA特性及其检测方法 被引量:7
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作者 胡永坚 王暄 李红梅 《南京农业大学学报》 CAS CSCD 北大核心 2007年第4期62-65,共4页
通过对rDNA的ITS区和IGS2区进行PCR扩增并对产物测序,比较分析了西班牙根结线虫与中国4种常见的南方根结线虫、爪哇根结线虫、花生根结线虫和北方根结线虫群体rDNA的ITS区和IGS2区的序列差异,选择限制性内切酶TaqⅠ对IGS2区的PCR扩增产... 通过对rDNA的ITS区和IGS2区进行PCR扩增并对产物测序,比较分析了西班牙根结线虫与中国4种常见的南方根结线虫、爪哇根结线虫、花生根结线虫和北方根结线虫群体rDNA的ITS区和IGS2区的序列差异,选择限制性内切酶TaqⅠ对IGS2区的PCR扩增产物进行酶切,获得了西班牙根结线虫的554 bp的特异性条带。表明用此方法可以明显将西班牙根结线虫与其他4种常见的根结线虫群体区分开,其灵敏度可达到单条根结线虫二龄幼虫。 展开更多
关键词 西班牙根结线虫 RDNA ITS IGS2 RFLP
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根结线虫在中国的新纪录种——西班牙根结线虫(Meloidogyne hispanica Hirschmann) 被引量:3
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作者 王暄 李红梅 +1 位作者 胡永坚 徐建华 《植物病理学报》 CAS CSCD 北大核心 2007年第3期321-324,共4页
A root-knot nematode, Meloidogyne hispanica Hirschmann, was first described and illustrated from roots of papaya in Danzhou, Hainan province in China. The perineal pattern of female was characterized by oval shaped to... A root-knot nematode, Meloidogyne hispanica Hirschmann, was first described and illustrated from roots of papaya in Danzhou, Hainan province in China. The perineal pattern of female was characterized by oval shaped to rectangular with a low dorsal arch; dorsal striae varied from fine and wavy to coarse; lateral lines forked with fringe-like striae between lines and phasmidial ducts distinctly appeared. Male had high and rounded head region with labial disc and medial lips fused to form elongate lip structures. Second-stage juve-nile had distinct body annules and stylet with rounded, posteriorly sloping knobs, and the tail terminus was slender with indistinct hyaline. This Chinese new record of M. hispanica had a unique esterase phenotype (S2-M1) which was different from that of other Meloidogyne species. 展开更多
关键词 根结线虫 新纪录种 西班牙 寄主植物 中国 植物检疫对象 病原线虫 二龄幼虫
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