This study was aimed to synthesize an active peptide from Carapax Trionycis and to investigate its effect on the proliferation of hepatic stellate cells（HSCs）.An active peptide from Carapax Trionycis,which was shown...This study was aimed to synthesize an active peptide from Carapax Trionycis and to investigate its effect on the proliferation of hepatic stellate cells（HSCs）.An active peptide from Carapax Trionycis,which was shown to have significant anti-hepatic fibrosis activity,was synthesized by solid phase method and characterized by MALDI-TOF MS analysis.The HSCs in log growth phase was treated with the synthetic peptide at different concentrations.Viability and apoptosis of hepatic stellate cells were determined by MTS assay and Annexin V-FITC/PI staining,respectively.The active peptide showed strong inhibition of proliferation and induction of apoptosis of HSC-T6 in a concentration-dependent manner.The results suggest that the active peptide from Carapax Trionycis could be synthesized efficiently and has significant inhibitory effect on the proliferation of HSC-T6.展开更多
目的比较3种药敏检测方法检测替加环素对多重耐药鲍曼不动杆菌敏感性的差异。方法收集2018年临床分离耐碳青霉烯类鲍曼不动杆菌60株,采用MIC Test Strip(MTS)法、VITEK-2法、纸片扩散法分别检测替加环素对耐碳青霉烯类鲍曼不动杆菌的敏...目的比较3种药敏检测方法检测替加环素对多重耐药鲍曼不动杆菌敏感性的差异。方法收集2018年临床分离耐碳青霉烯类鲍曼不动杆菌60株,采用MIC Test Strip(MTS)法、VITEK-2法、纸片扩散法分别检测替加环素对耐碳青霉烯类鲍曼不动杆菌的敏感性。结果按照FDA标准,以MTS法为参考,VITEK-2法的一致率较高,纸片扩散法的一致率较低。Vitek-2法的MIC值比MTS法的MIC值低1~2个稀释度,利用MTS法检测替加环素对耐碳青霉烯鲍曼不动杆菌的耐药率达到23.3%,出现耐药株。结论对于耐碳青霉烯类鲍曼不动杆菌,Vitek-2法和纸片扩散法均不适合检测替加环素的敏感性,不能作为常规方法,需用MTS法确认。展开更多
文摘This study was aimed to synthesize an active peptide from Carapax Trionycis and to investigate its effect on the proliferation of hepatic stellate cells（HSCs）.An active peptide from Carapax Trionycis,which was shown to have significant anti-hepatic fibrosis activity,was synthesized by solid phase method and characterized by MALDI-TOF MS analysis.The HSCs in log growth phase was treated with the synthetic peptide at different concentrations.Viability and apoptosis of hepatic stellate cells were determined by MTS assay and Annexin V-FITC/PI staining,respectively.The active peptide showed strong inhibition of proliferation and induction of apoptosis of HSC-T6 in a concentration-dependent manner.The results suggest that the active peptide from Carapax Trionycis could be synthesized efficiently and has significant inhibitory effect on the proliferation of HSC-T6.
文摘目的比较3种药敏检测方法检测替加环素对多重耐药鲍曼不动杆菌敏感性的差异。方法收集2018年临床分离耐碳青霉烯类鲍曼不动杆菌60株,采用MIC Test Strip(MTS)法、VITEK-2法、纸片扩散法分别检测替加环素对耐碳青霉烯类鲍曼不动杆菌的敏感性。结果按照FDA标准,以MTS法为参考,VITEK-2法的一致率较高,纸片扩散法的一致率较低。Vitek-2法的MIC值比MTS法的MIC值低1~2个稀释度,利用MTS法检测替加环素对耐碳青霉烯鲍曼不动杆菌的耐药率达到23.3%,出现耐药株。结论对于耐碳青霉烯类鲍曼不动杆菌,Vitek-2法和纸片扩散法均不适合检测替加环素的敏感性,不能作为常规方法,需用MTS法确认。
