Background and Objective:Chemotherapy is the main treatment for colon cancer,while multidrugresistance is the main reason for chemotherapy failure and tumor relapse.This study was to establish two oxaliplatinresistant...Background and Objective:Chemotherapy is the main treatment for colon cancer,while multidrugresistance is the main reason for chemotherapy failure and tumor relapse.This study was to establish two oxaliplatinresistant colon cancer cell lines and evaluate their biological characteristics.Met hods:Oxaliplatin-resistant colon cancer cell lines SW620/L-OHP and lovo/L-OHP were established in vitro by continuous exposure to oxaliplatin(L-OHP) of low and gradually increased concentration.Growth curve,cross-resistance and resistance index of the oxaliplatin-resistant cell lines to various anti-cancer agents were determined by CCK8 assay.The expressions of P-glycoprotein(P-gp),multidrugresistance protein 1(MRP1) and MRP2 were detected by Western blot.Cell cycle distribution as well as the expression of CD133 and CD44 were measured by flow cytometry.Result s:It took 10 months to establish the SW620/L-OHP and LoVo/L-OHP cell lines with stable resistance to oxaliplatin.Cross-resistance to 5-fluorouracil,etoposide,cisplatin,vincristine and epirubicin but not to paclitaxel was observed.Longer doubling time,higher proportion of cells in G0/G1 phase and lower proportion in G2/M phase were observed in the two oxaliplatin-resistant cell lines compared with their parental cell lines.The expression of MRP2 in the oxaliplatin-resistant cells was up-regulated,while those of P-gp and MRP1 had no significant change.CD133 was overexpressed while CD44 level remained unchanged in SW620/L-OHP and LoVo/L-OHP cells.Conclusions:SW620/L-OHP and LoVo/L-OHP cell lines show a typical and stably resistant phenotype and may be used as research models.展开更多
目的探讨胆盐载体MRP1、MRP2在妊娠期肝内胆汁淤积症(ICP)胎盘的表达,分析胎盘胆盐载体与ICP发病的关系。方法收集8例正常早孕妇女绒毛组织、7例正常中孕妇女胎盘组织、20例正常晚孕妇女(对照组)胎盘组织,以及20例ICP患者胎盘组织,检测...目的探讨胆盐载体MRP1、MRP2在妊娠期肝内胆汁淤积症(ICP)胎盘的表达,分析胎盘胆盐载体与ICP发病的关系。方法收集8例正常早孕妇女绒毛组织、7例正常中孕妇女胎盘组织、20例正常晚孕妇女(对照组)胎盘组织,以及20例ICP患者胎盘组织,检测收集组织中胆盐载体MRP1和MRP2 mRNA的表达。结果ICP和正常妊娠各期胎盘组织中均有MRP2 mRNA的表达,而MRP1 mRNA在大部分标本上有表达;ICP组MRP1 mRNA和MRP2 mRNA表达量与对照组相比(99.94±73.17 vs 99.20±68.65;95.78±56.50 vs 142.20±91.27)差异无统计学意义(P>0.05);ICP组未用地塞米松治疗胎盘MRP2 mRNA表达量低于对照组(91.82±48.08 vs 142.20±91.27,P<0.05)。结论在未用地塞米松治疗的ICP患者胎盘组织中,MRP2 mRNA的表达量降低,这可能是引起ICP患者胎盘胆汁酸转运障碍,从而引起胎儿体内胆汁淤积的机制之一。展开更多
基金National Natural Science Foundation of China (No. 30901719,No.30971519)
文摘Background and Objective:Chemotherapy is the main treatment for colon cancer,while multidrugresistance is the main reason for chemotherapy failure and tumor relapse.This study was to establish two oxaliplatinresistant colon cancer cell lines and evaluate their biological characteristics.Met hods:Oxaliplatin-resistant colon cancer cell lines SW620/L-OHP and lovo/L-OHP were established in vitro by continuous exposure to oxaliplatin(L-OHP) of low and gradually increased concentration.Growth curve,cross-resistance and resistance index of the oxaliplatin-resistant cell lines to various anti-cancer agents were determined by CCK8 assay.The expressions of P-glycoprotein(P-gp),multidrugresistance protein 1(MRP1) and MRP2 were detected by Western blot.Cell cycle distribution as well as the expression of CD133 and CD44 were measured by flow cytometry.Result s:It took 10 months to establish the SW620/L-OHP and LoVo/L-OHP cell lines with stable resistance to oxaliplatin.Cross-resistance to 5-fluorouracil,etoposide,cisplatin,vincristine and epirubicin but not to paclitaxel was observed.Longer doubling time,higher proportion of cells in G0/G1 phase and lower proportion in G2/M phase were observed in the two oxaliplatin-resistant cell lines compared with their parental cell lines.The expression of MRP2 in the oxaliplatin-resistant cells was up-regulated,while those of P-gp and MRP1 had no significant change.CD133 was overexpressed while CD44 level remained unchanged in SW620/L-OHP and LoVo/L-OHP cells.Conclusions:SW620/L-OHP and LoVo/L-OHP cell lines show a typical and stably resistant phenotype and may be used as research models.
文摘目的探讨胆盐载体MRP1、MRP2在妊娠期肝内胆汁淤积症(ICP)胎盘的表达,分析胎盘胆盐载体与ICP发病的关系。方法收集8例正常早孕妇女绒毛组织、7例正常中孕妇女胎盘组织、20例正常晚孕妇女(对照组)胎盘组织,以及20例ICP患者胎盘组织,检测收集组织中胆盐载体MRP1和MRP2 mRNA的表达。结果ICP和正常妊娠各期胎盘组织中均有MRP2 mRNA的表达,而MRP1 mRNA在大部分标本上有表达;ICP组MRP1 mRNA和MRP2 mRNA表达量与对照组相比(99.94±73.17 vs 99.20±68.65;95.78±56.50 vs 142.20±91.27)差异无统计学意义(P>0.05);ICP组未用地塞米松治疗胎盘MRP2 mRNA表达量低于对照组(91.82±48.08 vs 142.20±91.27,P<0.05)。结论在未用地塞米松治疗的ICP患者胎盘组织中,MRP2 mRNA的表达量降低,这可能是引起ICP患者胎盘胆汁酸转运障碍,从而引起胎儿体内胆汁淤积的机制之一。
