为了阐明草鱼MHC class I等位基因的结构与多态性,进一步研究其与疾病的关系,从草鱼cDNA文库中克隆了MHC class I基因(Ctid-MHC I);并通过对12个个体Ctid-MHC I的克隆,分析了其等位基因的多态性与三级结构.结果显示Ctid-MHC I等位基因在...为了阐明草鱼MHC class I等位基因的结构与多态性,进一步研究其与疾病的关系,从草鱼cDNA文库中克隆了MHC class I基因(Ctid-MHC I);并通过对12个个体Ctid-MHC I的克隆,分析了其等位基因的多态性与三级结构.结果显示Ctid-MHC I等位基因在α1与α2区域变异幅度大,可分为6类(Ctid-MHC I-UA-UF),9型(A-I);但是其三级结构和抗原多肽结合的关键性氨基酸十分保守.结果阐明了草鱼MHC class I分子在8个区域(A-H)置换率高,存在插入或缺失以及长度变异,使等位基因呈现高度多态性.动物MHC class I分子系统树也提示了我国大陆架上鱼类、两栖类、鸟类、哺乳动物和人的遗传距离与分枝年代.展开更多
Chronic HBV infection is associated with a 100-fold high risk of developing hepatocellular carcinoma. Tumor recognition is of the most importance during the immune surveillance process that prevents cancer development...Chronic HBV infection is associated with a 100-fold high risk of developing hepatocellular carcinoma. Tumor recognition is of the most importance during the immune surveillance process that prevents cancer development in humans. In the present study, the expressions of MHC class Ⅰ molecules on hepatoplastoma cell line HepG2.2.15 were investigated to indicate the possible effects of HBV on the immune recognition during HBV-associated hepatocellular carcinoma. It was found that the expressions of MHC class Ⅰ molecules HLA-ABC, HLA-E and MICA were much lower in HepG2.2.15 cells compared with HepG2 cells. The expressing HBV in human hepatoplastoma cell line significantly down-regulated the expressions of MHC class Ⅰ molecules. Additionally, it was observed that in murine chronic HBsAg carriers the expression of classical MHC-I molecule on hepatocytes was down-regulated. These results demonstrated that HBV might affect the immune recognition during HBV- associated hepatocellular carcinoma such as the recognition of CD8^＋ T, NK-CTL and NK cells and prevent the immune surveillance against tumors. However, the effects of HBV down-regulation of MHC class I molecules on the target cells in vivo should be further studied. Cellular ＆ Molecular Immunology.展开更多
文摘为了阐明草鱼MHC class I等位基因的结构与多态性,进一步研究其与疾病的关系,从草鱼cDNA文库中克隆了MHC class I基因(Ctid-MHC I);并通过对12个个体Ctid-MHC I的克隆,分析了其等位基因的多态性与三级结构.结果显示Ctid-MHC I等位基因在α1与α2区域变异幅度大,可分为6类(Ctid-MHC I-UA-UF),9型(A-I);但是其三级结构和抗原多肽结合的关键性氨基酸十分保守.结果阐明了草鱼MHC class I分子在8个区域(A-H)置换率高,存在插入或缺失以及长度变异,使等位基因呈现高度多态性.动物MHC class I分子系统树也提示了我国大陆架上鱼类、两栖类、鸟类、哺乳动物和人的遗传距离与分枝年代.
文摘Chronic HBV infection is associated with a 100-fold high risk of developing hepatocellular carcinoma. Tumor recognition is of the most importance during the immune surveillance process that prevents cancer development in humans. In the present study, the expressions of MHC class Ⅰ molecules on hepatoplastoma cell line HepG2.2.15 were investigated to indicate the possible effects of HBV on the immune recognition during HBV-associated hepatocellular carcinoma. It was found that the expressions of MHC class Ⅰ molecules HLA-ABC, HLA-E and MICA were much lower in HepG2.2.15 cells compared with HepG2 cells. The expressing HBV in human hepatoplastoma cell line significantly down-regulated the expressions of MHC class Ⅰ molecules. Additionally, it was observed that in murine chronic HBsAg carriers the expression of classical MHC-I molecule on hepatocytes was down-regulated. These results demonstrated that HBV might affect the immune recognition during HBV- associated hepatocellular carcinoma such as the recognition of CD8^＋ T, NK-CTL and NK cells and prevent the immune surveillance against tumors. However, the effects of HBV down-regulation of MHC class I molecules on the target cells in vivo should be further studied. Cellular ＆ Molecular Immunology.
