Background Id3 plays a key role in the progression of breast cancer. Previously, four and a half LIM protein （FHL2） was identified as a repressor of Id family proteins by interacting with them. This study aimed to i...Background Id3 plays a key role in the progression of breast cancer. Previously, four and a half LIM protein （FHL2） was identified as a repressor of Id family proteins by interacting with them. This study aimed to investigate the effects of FHL2 on the transcriptional regulation and oncogenic activities of Id3 in human breast cancer cells. Methods Cell transfection was performed with SuperFect reagent. Stable transfectants that overexpressed Id3 were obtained by selection on G418. The level of Id3 protein was determined by Western blotting analysis. Dual luciferase assays were used to measure the effect of Id3 and FHL2 on E47-mediated transcriptional activity in MCF-7 human breast cancer cells. The MTT assay was used to measure cell proliferation. The transwell assay was used to measure the invasive capacity of MCF-7 cancer cells. Results Id3 markedly repressed transcription mediated by the basic helix-loop-helix （bHLH） factor E47 in MCF-7 cells. This Id3-mediated repression was effectively antagonized by FHL2. Overexpression of Id3 markedly promoted the proliferation and invasive capacity of MCF-7 cells; however, these effects were significantly suppressed by the overexpression of FHL2. Conclusions FHL2 can inhibit the proliferation and invasive growth of human breast cancer cells by repressing the functional activity of Id3. The functional roles of FHL2-1d3 signaling in the development of human breast cancer need further research.展开更多
Objective:To investigate anticancer activity of different fractions of Tephrosia purpurea[TP] （Sharapunkha,Fabaceae） and Ficus religiosa[FR]（Peepal,Moraceae）.Methods:The fractions of TP and FR were prepared and te...Objective:To investigate anticancer activity of different fractions of Tephrosia purpurea[TP] （Sharapunkha,Fabaceae） and Ficus religiosa[FR]（Peepal,Moraceae）.Methods:The fractions of TP and FR were prepared and tested for in vitro anticancer activity using human MCF 7 cell line by trypan blue exclusion method.Results:The result showed that among all these fractions of TPI.TPIII.FRI and FRIII showed better anticancer activity compared to other fractions.The IC<sub>50</sub> value for TPI（152.4μM）,TPIII（158.71μM）.FRI（160.3μM） and for FRIII（222.7μM） was observed.Conclusions:The present study shows anticancer potential of TP and FR fractions in MCF 7 cell line.展开更多
Recently, space-division multiplexing （SDM） techniques using multi-core fiber （MCF） and few-mode fiber （FMF） have been introduced into optical fiber communication to increase transmission capacity. Two main type...Recently, space-division multiplexing （SDM） techniques using multi-core fiber （MCF） and few-mode fiber （FMF） have been introduced into optical fiber communication to increase transmission capacity. Two main types of optical fiber amplifiers based on the Erbium- doped fiber （EDF） and the Raman effect have been developed to amplify signals in the MCF and FMF. In this paper, we reviewed the principles and configurations of these amplifiers.展开更多
Space-based plasma(i.e.,a highly ionized gas or the fourth state of matter)blobs are isolated pockets of this highly ionized gas made up of charged particles.These blobs are believed to have a substantial impact on th...Space-based plasma(i.e.,a highly ionized gas or the fourth state of matter)blobs are isolated pockets of this highly ionized gas made up of charged particles.These blobs are believed to have a substantial impact on the structure and dynamics of the cosmos and can be seen in a variety of astronomical objects,including stars,galaxies,and the intergalactic medium.Some plasma blobs are connected to intense phenomena like magnetic reconnection,shock waves,and supernovae,while others may be the result of more passive processes like cooling and gravitational collapse.In both astrophysics and plasma physics,there is ongoing research on the characteristics and behavior of plasma blobs.This phenomenon has a very adverse effect on tokamak-based MCF(magnetic confinement fusion),which is the subject of this short review paper.展开更多
Micron-sized hollow silica spheres whose shells are made up of mesocellular silica foams （MCFs） have been synthesized by one-pot sol-gel method in benzene/water/P123 emulsion. The material is characterized with SEM,...Micron-sized hollow silica spheres whose shells are made up of mesocellular silica foams （MCFs） have been synthesized by one-pot sol-gel method in benzene/water/P123 emulsion. The material is characterized with SEM, TEM, BET and ^29Si MAS NMR. The results show that the MCFs of the unique shell of hollow silica spheres were connected by large windows with a narrow distribution of - 10 nm in diameter, the inner space of the hollow sphere is accessible. And the formation mechanism of the hollow silica spheres is proposed. C 2009 Xin Wen Guo. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All rights reserved.展开更多
基金This work was supported by the National Natural Science Foundation of China （No. 30870507 and No. 81001184） and partially supported by a grant from the Ministry of Science and Technology of China （No. 2010CB912802）.
文摘Background Id3 plays a key role in the progression of breast cancer. Previously, four and a half LIM protein （FHL2） was identified as a repressor of Id family proteins by interacting with them. This study aimed to investigate the effects of FHL2 on the transcriptional regulation and oncogenic activities of Id3 in human breast cancer cells. Methods Cell transfection was performed with SuperFect reagent. Stable transfectants that overexpressed Id3 were obtained by selection on G418. The level of Id3 protein was determined by Western blotting analysis. Dual luciferase assays were used to measure the effect of Id3 and FHL2 on E47-mediated transcriptional activity in MCF-7 human breast cancer cells. The MTT assay was used to measure cell proliferation. The transwell assay was used to measure the invasive capacity of MCF-7 cancer cells. Results Id3 markedly repressed transcription mediated by the basic helix-loop-helix （bHLH） factor E47 in MCF-7 cells. This Id3-mediated repression was effectively antagonized by FHL2. Overexpression of Id3 markedly promoted the proliferation and invasive capacity of MCF-7 cells; however, these effects were significantly suppressed by the overexpression of FHL2. Conclusions FHL2 can inhibit the proliferation and invasive growth of human breast cancer cells by repressing the functional activity of Id3. The functional roles of FHL2-1d3 signaling in the development of human breast cancer need further research.
文摘Objective:To investigate anticancer activity of different fractions of Tephrosia purpurea[TP] （Sharapunkha,Fabaceae） and Ficus religiosa[FR]（Peepal,Moraceae）.Methods:The fractions of TP and FR were prepared and tested for in vitro anticancer activity using human MCF 7 cell line by trypan blue exclusion method.Results:The result showed that among all these fractions of TPI.TPIII.FRI and FRIII showed better anticancer activity compared to other fractions.The IC<sub>50</sub> value for TPI（152.4μM）,TPIII（158.71μM）.FRI（160.3μM） and for FRIII（222.7μM） was observed.Conclusions:The present study shows anticancer potential of TP and FR fractions in MCF 7 cell line.
文摘Recently, space-division multiplexing （SDM） techniques using multi-core fiber （MCF） and few-mode fiber （FMF） have been introduced into optical fiber communication to increase transmission capacity. Two main types of optical fiber amplifiers based on the Erbium- doped fiber （EDF） and the Raman effect have been developed to amplify signals in the MCF and FMF. In this paper, we reviewed the principles and configurations of these amplifiers.
文摘Space-based plasma(i.e.,a highly ionized gas or the fourth state of matter)blobs are isolated pockets of this highly ionized gas made up of charged particles.These blobs are believed to have a substantial impact on the structure and dynamics of the cosmos and can be seen in a variety of astronomical objects,including stars,galaxies,and the intergalactic medium.Some plasma blobs are connected to intense phenomena like magnetic reconnection,shock waves,and supernovae,while others may be the result of more passive processes like cooling and gravitational collapse.In both astrophysics and plasma physics,there is ongoing research on the characteristics and behavior of plasma blobs.This phenomenon has a very adverse effect on tokamak-based MCF(magnetic confinement fusion),which is the subject of this short review paper.
基金sponsored by the Program for New Century Excellent Talents in the University(No.NCET-04-0268)by the 111 Project.
文摘Micron-sized hollow silica spheres whose shells are made up of mesocellular silica foams （MCFs） have been synthesized by one-pot sol-gel method in benzene/water/P123 emulsion. The material is characterized with SEM, TEM, BET and ^29Si MAS NMR. The results show that the MCFs of the unique shell of hollow silica spheres were connected by large windows with a narrow distribution of - 10 nm in diameter, the inner space of the hollow sphere is accessible. And the formation mechanism of the hollow silica spheres is proposed. C 2009 Xin Wen Guo. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All rights reserved.
