采用小鼠Ⅲ度烧伤模型,观察了溶葡萄球菌酶对烧伤后腹腔巨噬细胞吞噬、消化鸡红细胞能力,化学发光强度以及全身碳末廓清功能的影响,旨在探讨溶葡萄球菌酶改善机体抗感染能力的作用。结果表明:烧伤后小鼠腹腔巨噬细胞吞噬消化能力和化学...采用小鼠Ⅲ度烧伤模型,观察了溶葡萄球菌酶对烧伤后腹腔巨噬细胞吞噬、消化鸡红细胞能力,化学发光强度以及全身碳末廓清功能的影响,旨在探讨溶葡萄球菌酶改善机体抗感染能力的作用。结果表明:烧伤后小鼠腹腔巨噬细胞吞噬消化能力和化学发光强度均明显降低;碳末廓清功能明显受损。给予溶葡萄球菌酶后,小鼠腹腔巨噬细胞吞噬和消化鸡红细胞能力明显改善,化学发光强度增强,并超过烧伤组和正常对照组。同时溶葡萄球菌酶还可增强全身碳末廓清功能,其 a 值可达1.63×10^(-2),显著高于烧伤组(8.13×10^(-4))和正常对照组(2.76×10^(-3))。提示溶葡萄球菌酶具有增强吞噬细胞功能,进而改善机体抗感染能力的作用。展开更多
目的研究溶葡萄球菌酶在体外和创面肉芽组织中对金黄色葡萄球菌等几种有代表性的常见创面感染菌的抗菌效果。方法用体外药敏试验观察了溶葡萄球菌酶对金黄色葡萄球菌、白色念珠菌和铜绿假单孢菌抗菌效果；并用组织细菌定量分析的方法分...目的研究溶葡萄球菌酶在体外和创面肉芽组织中对金黄色葡萄球菌等几种有代表性的常见创面感染菌的抗菌效果。方法用体外药敏试验观察了溶葡萄球菌酶对金黄色葡萄球菌、白色念珠菌和铜绿假单孢菌抗菌效果；并用组织细菌定量分析的方法分析了溶葡萄球菌酶对29例Ⅲ度面积10％～30％总体表面积（total body Surface area，TBSA）的烧伤患者残留创面肉芽组织中金黄色葡萄球菌的抗菌效果。结果溶葡萄球菌酶对金黄色葡萄球菌有很强的抗菌效果，对白色念珠菌也有明显的抗菌效果；对铜绿假单孢菌的抗菌效果较差。溶葡萄球菌酶纱布在烧伤残留创面连续使用3d后，能杀死肉芽组织中89．2％的细菌。证明溶葡萄球菌酶能有效控制肉芽组织中的细菌数量。同时，杀菌纱布所含成分是生物酶类，使用后未见全身不良反应。结论溶葡萄球菌酶除了对金黄色葡萄球菌有很强的杀菌作用外，对白色念珠菌也有很好的杀菌作用，溶葡萄球菌酶可以作为控制中晚期烧伤残余创面金黄色葡萄球菌感染非常有效的局部用药。展开更多
Lysostaphin is widely used in clinical settings against Staphylococcus aureus, but its mutants can abolish its killing activity. The difficulty in studies of mutations in lysostaphin is the shortage of data, which may...Lysostaphin is widely used in clinical settings against Staphylococcus aureus, but its mutants can abolish its killing activity. The difficulty in studies of mutations in lysostaphin is the shortage of data, which may need many decades to collect, although lysostaphin is so important for clinical therapeutics and drug development. In order not to passively wait for the accumulation of new data, in this study 1) the 23,442 mutations in 1408 proteins from databank were used to determine whether the mutations in lysostaphin follow the general mutation trend obtained from the databank, 2) the amino-acid pair predictability was used to explore the underlined mechanism for lysostaphin mutations, and 3) the amino-acid distribution probability was used to associate the mutation with dysfunction of lysostaphin. The results show that the mutations in lysostaphin follow the general trend of mutations in proteins;the underlined mechanism for mutations in lysostaphin is explainable from a viewpoint of randomness, and a mutation with increased distribution probability would have a larger chance to dysfunction lysostaphin. This study provides useful information for future design of anti-S. aureus drug and enzyme engineering.展开更多
文摘采用小鼠Ⅲ度烧伤模型,观察了溶葡萄球菌酶对烧伤后腹腔巨噬细胞吞噬、消化鸡红细胞能力,化学发光强度以及全身碳末廓清功能的影响,旨在探讨溶葡萄球菌酶改善机体抗感染能力的作用。结果表明:烧伤后小鼠腹腔巨噬细胞吞噬消化能力和化学发光强度均明显降低;碳末廓清功能明显受损。给予溶葡萄球菌酶后,小鼠腹腔巨噬细胞吞噬和消化鸡红细胞能力明显改善,化学发光强度增强,并超过烧伤组和正常对照组。同时溶葡萄球菌酶还可增强全身碳末廓清功能,其 a 值可达1.63×10^(-2),显著高于烧伤组(8.13×10^(-4))和正常对照组(2.76×10^(-3))。提示溶葡萄球菌酶具有增强吞噬细胞功能,进而改善机体抗感染能力的作用。
文摘目的研究溶葡萄球菌酶在体外和创面肉芽组织中对金黄色葡萄球菌等几种有代表性的常见创面感染菌的抗菌效果。方法用体外药敏试验观察了溶葡萄球菌酶对金黄色葡萄球菌、白色念珠菌和铜绿假单孢菌抗菌效果；并用组织细菌定量分析的方法分析了溶葡萄球菌酶对29例Ⅲ度面积10％～30％总体表面积（total body Surface area，TBSA）的烧伤患者残留创面肉芽组织中金黄色葡萄球菌的抗菌效果。结果溶葡萄球菌酶对金黄色葡萄球菌有很强的抗菌效果，对白色念珠菌也有明显的抗菌效果；对铜绿假单孢菌的抗菌效果较差。溶葡萄球菌酶纱布在烧伤残留创面连续使用3d后，能杀死肉芽组织中89．2％的细菌。证明溶葡萄球菌酶能有效控制肉芽组织中的细菌数量。同时，杀菌纱布所含成分是生物酶类，使用后未见全身不良反应。结论溶葡萄球菌酶除了对金黄色葡萄球菌有很强的杀菌作用外，对白色念珠菌也有很好的杀菌作用，溶葡萄球菌酶可以作为控制中晚期烧伤残余创面金黄色葡萄球菌感染非常有效的局部用药。
文摘Lysostaphin is widely used in clinical settings against Staphylococcus aureus, but its mutants can abolish its killing activity. The difficulty in studies of mutations in lysostaphin is the shortage of data, which may need many decades to collect, although lysostaphin is so important for clinical therapeutics and drug development. In order not to passively wait for the accumulation of new data, in this study 1) the 23,442 mutations in 1408 proteins from databank were used to determine whether the mutations in lysostaphin follow the general mutation trend obtained from the databank, 2) the amino-acid pair predictability was used to explore the underlined mechanism for lysostaphin mutations, and 3) the amino-acid distribution probability was used to associate the mutation with dysfunction of lysostaphin. The results show that the mutations in lysostaphin follow the general trend of mutations in proteins;the underlined mechanism for mutations in lysostaphin is explainable from a viewpoint of randomness, and a mutation with increased distribution probability would have a larger chance to dysfunction lysostaphin. This study provides useful information for future design of anti-S. aureus drug and enzyme engineering.
