The emerging food additive, xylo-oligosaccharide(XOSs), was prepared through enzymatic hydrolysis of hemicelluloses isolated from press lye. Two of the three experimental enzyme preparations presented favourable effec...The emerging food additive, xylo-oligosaccharide(XOSs), was prepared through enzymatic hydrolysis of hemicelluloses isolated from press lye. Two of the three experimental enzyme preparations presented favourable effects, while the other one consisting of the nominal enzyme preparations with high enzyme activity was found to have poor catalytic effects. The conversion of xylan exceeded 40% and the selectivity for XOSs reached 90% when the temperature, hemicellulose concentration, dosage of enzymes, and hydrolysis time were 40?C, 100 g/L, 1 ku/g, and 4 h, respectively. Xylo-oligosaccharide preparation through enzymatic hydrolysis of hemicelluloses isolated from press lye was proved to be a feasible process to utilize the by-product of the lye regeneration, which will substantially improve the economy of the lye regeneration and recycling.展开更多
[Objectives] This study was conducted to optimize the extraction process of Sipunculus nudus polysaccharide. [Methods]S. nudus polysaccharide was extracted by ultrasonic water extraction,with the marine S. nudus as a ...[Objectives] This study was conducted to optimize the extraction process of Sipunculus nudus polysaccharide. [Methods]S. nudus polysaccharide was extracted by ultrasonic water extraction,with the marine S. nudus as a raw material from Sanya City,Hainan Province,by single factor variable method,to find the optimal extraction conditions including ultrasonic power,solid-liquid ratio,ultrasonic extraction temperature,ultrasonic extraction time,ultrasonic extraction times and lye concentration. The absorbance of the polysaccharide was determined by an ultraviolet spectrophotometer,and polysaccharide yield were calculated. The antioxidant activity of scavenging hydroxyl and DPPH radicals was measured at different concentrations. [Results]The optimal extraction conditions for S. nudus polysaccharide were as follows： solid-liquid ratio at 1∶11 mg/ml,extraction temperature at 60 ℃,three times of ultrasonic extraction,ultrasonic power of 960 W,extraction time of 1. 5 h,and lye concentration of 5%,under which the polysaccharide yield was 3. 21%. The scavenging rates of hydroxyl radical and DPPH radical were 12. 58% at the concentration of 0. 20 mg/ml. Moreover,the scavenging rates increased significantly with the increase of concentration. [Conclusions] This method is a new trial with satisfactory results.展开更多
基金Supported by Science and Education Integration Program of Henan University of Technology
文摘The emerging food additive, xylo-oligosaccharide(XOSs), was prepared through enzymatic hydrolysis of hemicelluloses isolated from press lye. Two of the three experimental enzyme preparations presented favourable effects, while the other one consisting of the nominal enzyme preparations with high enzyme activity was found to have poor catalytic effects. The conversion of xylan exceeded 40% and the selectivity for XOSs reached 90% when the temperature, hemicellulose concentration, dosage of enzymes, and hydrolysis time were 40?C, 100 g/L, 1 ku/g, and 4 h, respectively. Xylo-oligosaccharide preparation through enzymatic hydrolysis of hemicelluloses isolated from press lye was proved to be a feasible process to utilize the by-product of the lye regeneration, which will substantially improve the economy of the lye regeneration and recycling.
基金Supported by National Natural Science Foundation of Hainan Province(217157)Fund for Construction of Provincial Key Discipline(thalassochemistry)in 2017
文摘[Objectives] This study was conducted to optimize the extraction process of Sipunculus nudus polysaccharide. [Methods]S. nudus polysaccharide was extracted by ultrasonic water extraction,with the marine S. nudus as a raw material from Sanya City,Hainan Province,by single factor variable method,to find the optimal extraction conditions including ultrasonic power,solid-liquid ratio,ultrasonic extraction temperature,ultrasonic extraction time,ultrasonic extraction times and lye concentration. The absorbance of the polysaccharide was determined by an ultraviolet spectrophotometer,and polysaccharide yield were calculated. The antioxidant activity of scavenging hydroxyl and DPPH radicals was measured at different concentrations. [Results]The optimal extraction conditions for S. nudus polysaccharide were as follows： solid-liquid ratio at 1∶11 mg/ml,extraction temperature at 60 ℃,three times of ultrasonic extraction,ultrasonic power of 960 W,extraction time of 1. 5 h,and lye concentration of 5%,under which the polysaccharide yield was 3. 21%. The scavenging rates of hydroxyl radical and DPPH radical were 12. 58% at the concentration of 0. 20 mg/ml. Moreover,the scavenging rates increased significantly with the increase of concentration. [Conclusions] This method is a new trial with satisfactory results.
