Objective:Gastrointestinal heat retention syndrome(GHRS)is associated with lung-heat syndrome and is related to recurrent respiratory infection.Upper respiratory tract infection(URTI)lung heat syndrome is common in ch...Objective:Gastrointestinal heat retention syndrome(GHRS)is associated with lung-heat syndrome and is related to recurrent respiratory infection.Upper respiratory tract infection(URTI)lung heat syndrome is common in children.The study will explore the effect of GHRS on the structure and function of gut microbiota in children with URTI lung-heat syndrome.Methods:Participants were divided into both groups using the self-developed URTI scale and the“GHRS Diagnostic Scale$Pediatric Part”:GHRS-positive children(LS group)and GHRS-negative children(L group).General information,clinical symptoms,and stool were collected.We used 16S rRNA amplicon sequencing technology to determine the gene sequence of the V3eV4 region in feces and measure the gut microbiota of the both groups at the genus level.Results:A total of 23 children were included in the both groups.There were 12 cases in the LS group and 11 cases in the L group.There was no statistical difference between the both groups in age,gender,height,weight,and body mass index.The effective sequences shared by the both groups accounted for 85.66%of the total.In the gut microbiota,there was no difference in the a diversity and the b diversity between the both groups.Compared with the L group,the LS group had a significant increase in the relative abundance of the Ruminococcus gnavus group,Prevotella-9,Staphylococcus,and Actinomyces(P<.05).The functions of the both groups of microbiota primarily concentrate on metabolism,genetic information processing,and environmental information processing.The relative abundance of signaling molecules and interactions in the LS group were higher than that in the L group(P<.05).The redundancy analysis(RDA)showed that the URTI score had the greatest impact on the distribution of microbiota.Conclusion:GHRS may affect the development of URTI lung-heat syndrome by changing the relative abundances of gut microbiota.展开更多
目的评价自拟凉血清肺汤联合西医常规疗法治疗玫瑰痤疮肺胃热盛证临床疗效。方法将符合入选标准的2017年5月-2019年5月本院玫瑰痤疮患者312例采用随机数字表法分为2组,每组156例。对照组口服盐酸多西环素片并配合外敷甲硝唑凝胶治疗,研...目的评价自拟凉血清肺汤联合西医常规疗法治疗玫瑰痤疮肺胃热盛证临床疗效。方法将符合入选标准的2017年5月-2019年5月本院玫瑰痤疮患者312例采用随机数字表法分为2组,每组156例。对照组口服盐酸多西环素片并配合外敷甲硝唑凝胶治疗,研究组在对照组基础上加服自拟凉血清肺汤。2组均治疗4周。采用皮肤病生活质量指数(Dermatology Life Quality Index,DLQI)评价患者的生活质量,采用多功能皮肤测试仪测量皮损区红斑指数(erythema index,EI),记录治疗期间的不良反应,评价临床疗效。结果研究组总有效率为80.8%(126/156)、对照组为66.7%(104/156),2组比较差异有统计学意义(χ^(2)=8.012,P=0.005)。治疗后,研究组生理、心理、家庭、社会活动、人际交往和职业限制、治疗评分均低于对照组(t值分别为17.942、15.794、19.465、21.586、31.375、27.472,P值均<0.001);EI[(619.69±71.53)比(822.97±89.47),t=32.348]低于对照组(P<0.01)。治疗期间,对照组不良反应发生率为4.5%(7/156)、研究组为5.8%(9/156),2组比较差异无统计学意义(χ^(2)=0.439,P=0.514)。结论自拟凉血清肺汤联合西医常规疗法可有效改善玫瑰痤疮肺胃热盛证患者的临床症状及生活质量,提高临床疗效且安全性较好。展开更多
基金This study was financially supported by the National Key R&D Program of China(2018YFC1704100 and 2018YFC1704101)Beijing Municipal Natural Science Foundation(7172131).
文摘Objective:Gastrointestinal heat retention syndrome(GHRS)is associated with lung-heat syndrome and is related to recurrent respiratory infection.Upper respiratory tract infection(URTI)lung heat syndrome is common in children.The study will explore the effect of GHRS on the structure and function of gut microbiota in children with URTI lung-heat syndrome.Methods:Participants were divided into both groups using the self-developed URTI scale and the“GHRS Diagnostic Scale$Pediatric Part”:GHRS-positive children(LS group)and GHRS-negative children(L group).General information,clinical symptoms,and stool were collected.We used 16S rRNA amplicon sequencing technology to determine the gene sequence of the V3eV4 region in feces and measure the gut microbiota of the both groups at the genus level.Results:A total of 23 children were included in the both groups.There were 12 cases in the LS group and 11 cases in the L group.There was no statistical difference between the both groups in age,gender,height,weight,and body mass index.The effective sequences shared by the both groups accounted for 85.66%of the total.In the gut microbiota,there was no difference in the a diversity and the b diversity between the both groups.Compared with the L group,the LS group had a significant increase in the relative abundance of the Ruminococcus gnavus group,Prevotella-9,Staphylococcus,and Actinomyces(P<.05).The functions of the both groups of microbiota primarily concentrate on metabolism,genetic information processing,and environmental information processing.The relative abundance of signaling molecules and interactions in the LS group were higher than that in the L group(P<.05).The redundancy analysis(RDA)showed that the URTI score had the greatest impact on the distribution of microbiota.Conclusion:GHRS may affect the development of URTI lung-heat syndrome by changing the relative abundances of gut microbiota.
文摘目的评价自拟凉血清肺汤联合西医常规疗法治疗玫瑰痤疮肺胃热盛证临床疗效。方法将符合入选标准的2017年5月-2019年5月本院玫瑰痤疮患者312例采用随机数字表法分为2组,每组156例。对照组口服盐酸多西环素片并配合外敷甲硝唑凝胶治疗,研究组在对照组基础上加服自拟凉血清肺汤。2组均治疗4周。采用皮肤病生活质量指数(Dermatology Life Quality Index,DLQI)评价患者的生活质量,采用多功能皮肤测试仪测量皮损区红斑指数(erythema index,EI),记录治疗期间的不良反应,评价临床疗效。结果研究组总有效率为80.8%(126/156)、对照组为66.7%(104/156),2组比较差异有统计学意义(χ^(2)=8.012,P=0.005)。治疗后,研究组生理、心理、家庭、社会活动、人际交往和职业限制、治疗评分均低于对照组(t值分别为17.942、15.794、19.465、21.586、31.375、27.472,P值均<0.001);EI[(619.69±71.53)比(822.97±89.47),t=32.348]低于对照组(P<0.01)。治疗期间,对照组不良反应发生率为4.5%(7/156)、研究组为5.8%(9/156),2组比较差异无统计学意义(χ^(2)=0.439,P=0.514)。结论自拟凉血清肺汤联合西医常规疗法可有效改善玫瑰痤疮肺胃热盛证患者的临床症状及生活质量,提高临床疗效且安全性较好。
