Background Immunosuppressive regulatory T cells （Tregs） participate in tumor immune evasion and the number and suppressive function of Tregs change with the aging process, but it is not clear whether such change lea...Background Immunosuppressive regulatory T cells （Tregs） participate in tumor immune evasion and the number and suppressive function of Tregs change with the aging process, but it is not clear whether such change leads to a higher incidence of tumors in the elderly. To this end, we designed experiments to explore the changes of Tregs and the functional gene Forkhead box P3 （FoxP3） in the aging process and its relationship with lung tumors in humans and mice. Methods The percentage of CD4＋CD25＋CD127lowTregs and expression of FoxP3 mRNA were analyzed using flow cytometry （FCM） and real-time fluorescence-based quantitative polymerase chain reaction （FQ-PCR）. Markers were analyzed in the peripheral blood （PB） of 65 elderly patients （age 〉-65 years） with primary non-small cell lung cancer （NSCLC）, 20 younger patients （aged 〈55 years） with NSCLC, 30 elderly healthy individuals and 30 young healthy individuals. Furthermore, we set up the Lewis lung cancer model with C57BL/6 female mice. Thirty-six mice were divided into a young healthy group, a middle-aged healthy group, tumor group, and an elderly tumor group. The percentage an elderly healthy group, a young tumor group, a middle-aged of CD4＋CD25＋FoxP3＋ Tregs and the expression level of FoxP3 mRNA in splenocytes were determined in the six groups. Results The percentage of peripheral CD4＋CD25＋CD127low Tregs and the expression of FoxP3 mRNA were significantly increased in elderly patients with NSCLC comparing with the other groups and in elderly healthy individuals compared with young healthy individuals. Further analysis showed that the percentage of CD4＋CD25＋CD127lowTregs and the expression of FoxP3 mRNA were closely associated with tumor node metastasis （TNM） staging in elderly patients with NSCLC. In the mouse model, the percentage of CD4＋CD25＋FoxP3＋ Tregs and the expression of FoxP3 mRNA in splenocytes of the tumor groups were significantly higher than in the healthy groups, with the highest expression i展开更多
Background:Cancer has become a global health problem,and assessments of cancer mortality are important for effective public health policy-making and adequate resource allocation.In this study,we aimed to predict the m...Background:Cancer has become a global health problem,and assessments of cancer mortality are important for effective public health policy-making and adequate resource allocation.In this study,we aimed to predict the mortality rates and numbers of deaths related to four common cancers(lung,liver,stomach,and esophagus)in China from 2020 to 2030 and to estimate the corresponding cancer burden caused by population aging and tobacco smoking.Methods:Cancer mortality data(2004-2017)were extracted from China’s death surveillance datasets,and China’s population figures(2020-2030)were obtained from the United Nations population projections.Smoking prevalence data were retrieved from a World Health Organization global report,and relative risks of smoking and cancers were derived from large-scale Asian studies.We predicted the deaths related to the four major cancers and age-standardized mortality rates using joinpoint regression and linear regression models.The tobacco smoking related burden of these four major cancers was estimated using the population attributable fraction.Results:Unlike lung cancer mortality which was predicted to continue to increase,the age-standardized mortality rates for digestive cancers(liver,stomach,and esophageal cancers)are predicted to decline over the next decade.The number of deaths caused by the four major cancers is predicted to increase from 1,490,304 in 2020 to 1,823,960 in 2030.The age-specific mortality rates of the four major cancers are predicted to increase with age after 40-45 years,peaking in the age groups of 80-84 and ≥85 years.In 2030,the combined number of deaths from the four examined cancers among adults aged ≥65 years is predicted to be 1,167,153,accounting for 64% of all deaths from these cancers.Tobacco smoking is predicted to contribute to nearly 29% of deaths fromthese cancers,corresponding to 527,577 deaths.Conclusions:The overall trend in the combined total mortality from four major cancers is predicted to decline over the next decade;however,the corresponding 展开更多
Zhao Cui-ying, researcher, female, born in 1940, doctoral supervisor, is an instructor at the research studio of Shanghai famous and senior Chinese medical practitioners＇ academic experience, used to enjoy the specia...Zhao Cui-ying, researcher, female, born in 1940, doctoral supervisor, is an instructor at the research studio of Shanghai famous and senior Chinese medical practitioners＇ academic experience, used to enjoy the special allowance of State Council. She graduated in 1964 from the Department of Medicine, Shanghai Second Medical University, and at the same year came to work in Shanghai Research Institute of Acupuncture and Meridian up to today, used to be a director of the Immunology Research Room of Shanghai Research Institute of Acupuncture and Meridian, and a consultant of Grade 3 Laboratory of Acupuncture Immunology of State Administration of Traditional Chinese Medicine, and is a vice chairwoman and deputy secretary general of Shanghai Professional Committee for the Respiratory Diseases of Integrated Chinese and Western Medicine. Zhao Cui-ying has been engaged in the clinical, research and teaching work in the field of immune-related diseases treated by acupuncture and moxibustion for over 40 years, with abundant and distinguished experience, has undertaken over 10 state and provincial subjects and offered many important viewpoints in the research of acupuncture treatment of immune-related diseases, and has obtained several scientific and technological achievements, and published totally 80 academic papers, and has been honored with 10 awards, including 6 provincial awards. As a tutor or instructor, she has trained 7 doctoral students and 16 master students.展开更多
SGDWSDIGGR(S-10-R)is a kind of antioxidant peptide derived from broken rice.In the present study,the effects of S-10-R against H_(2)O_(2)-induced cell damage and premature senescence in 2BS cells were determined.The r...SGDWSDIGGR(S-10-R)is a kind of antioxidant peptide derived from broken rice.In the present study,the effects of S-10-R against H_(2)O_(2)-induced cell damage and premature senescence in 2BS cells were determined.The results indicated that S-10-R could improve cell viability and mitochondrial membrane potential at least 1.06 and 1.12 folds compared with senescence cells.Moreover,S-10-R inhibitedβ-galactosidase,and DNA damage alleviates the effect on 2BS senescence cells due to scavenging ROS.The apoptosis rate can be reduced by 80.58%.Furthermore,S-10-R could inhibit PI3K and Akt phosphorylation,downregulate Bax and caspases-3 expression,and inhibit premature senescence through activating PI3K/Akt related intrinsic apoptotic signaling pathways and suppressing JNK/Bcl-2 related mitochondria-dependent apoptosis.The present study confirmed the feasibility of antioxidants as anti-aging drugs,as well as provided a substantial basis for future application of S-10-R as a functional ingredient against oxidative stress-induced premature aging.展开更多
文摘Background Immunosuppressive regulatory T cells （Tregs） participate in tumor immune evasion and the number and suppressive function of Tregs change with the aging process, but it is not clear whether such change leads to a higher incidence of tumors in the elderly. To this end, we designed experiments to explore the changes of Tregs and the functional gene Forkhead box P3 （FoxP3） in the aging process and its relationship with lung tumors in humans and mice. Methods The percentage of CD4＋CD25＋CD127lowTregs and expression of FoxP3 mRNA were analyzed using flow cytometry （FCM） and real-time fluorescence-based quantitative polymerase chain reaction （FQ-PCR）. Markers were analyzed in the peripheral blood （PB） of 65 elderly patients （age 〉-65 years） with primary non-small cell lung cancer （NSCLC）, 20 younger patients （aged 〈55 years） with NSCLC, 30 elderly healthy individuals and 30 young healthy individuals. Furthermore, we set up the Lewis lung cancer model with C57BL/6 female mice. Thirty-six mice were divided into a young healthy group, a middle-aged healthy group, tumor group, and an elderly tumor group. The percentage an elderly healthy group, a young tumor group, a middle-aged of CD4＋CD25＋FoxP3＋ Tregs and the expression level of FoxP3 mRNA in splenocytes were determined in the six groups. Results The percentage of peripheral CD4＋CD25＋CD127low Tregs and the expression of FoxP3 mRNA were significantly increased in elderly patients with NSCLC comparing with the other groups and in elderly healthy individuals compared with young healthy individuals. Further analysis showed that the percentage of CD4＋CD25＋CD127lowTregs and the expression of FoxP3 mRNA were closely associated with tumor node metastasis （TNM） staging in elderly patients with NSCLC. In the mouse model, the percentage of CD4＋CD25＋FoxP3＋ Tregs and the expression of FoxP3 mRNA in splenocytes of the tumor groups were significantly higher than in the healthy groups, with the highest expression i
基金Chinese Academy of Medical Sciences Innovation Fund for Medical Sciences,Grant/Award Number:2017-I2M-1-009Peking Union Medical College Innovation Fund for Graduate Students,Grant/Award Number:2018-1002-01-21.
文摘Background:Cancer has become a global health problem,and assessments of cancer mortality are important for effective public health policy-making and adequate resource allocation.In this study,we aimed to predict the mortality rates and numbers of deaths related to four common cancers(lung,liver,stomach,and esophagus)in China from 2020 to 2030 and to estimate the corresponding cancer burden caused by population aging and tobacco smoking.Methods:Cancer mortality data(2004-2017)were extracted from China’s death surveillance datasets,and China’s population figures(2020-2030)were obtained from the United Nations population projections.Smoking prevalence data were retrieved from a World Health Organization global report,and relative risks of smoking and cancers were derived from large-scale Asian studies.We predicted the deaths related to the four major cancers and age-standardized mortality rates using joinpoint regression and linear regression models.The tobacco smoking related burden of these four major cancers was estimated using the population attributable fraction.Results:Unlike lung cancer mortality which was predicted to continue to increase,the age-standardized mortality rates for digestive cancers(liver,stomach,and esophageal cancers)are predicted to decline over the next decade.The number of deaths caused by the four major cancers is predicted to increase from 1,490,304 in 2020 to 1,823,960 in 2030.The age-specific mortality rates of the four major cancers are predicted to increase with age after 40-45 years,peaking in the age groups of 80-84 and ≥85 years.In 2030,the combined number of deaths from the four examined cancers among adults aged ≥65 years is predicted to be 1,167,153,accounting for 64% of all deaths from these cancers.Tobacco smoking is predicted to contribute to nearly 29% of deaths fromthese cancers,corresponding to 527,577 deaths.Conclusions:The overall trend in the combined total mortality from four major cancers is predicted to decline over the next decade;however,the corresponding
基金supported by Scientific Research Budget Project of Shanghai Municipal Education Commission (09jw55)Special Scientific Research Fund for Selection and Cultivation of Elite in College and University (szy10071)National Basic Research Program of China (973 Program, 2009CB522900)
文摘Zhao Cui-ying, researcher, female, born in 1940, doctoral supervisor, is an instructor at the research studio of Shanghai famous and senior Chinese medical practitioners＇ academic experience, used to enjoy the special allowance of State Council. She graduated in 1964 from the Department of Medicine, Shanghai Second Medical University, and at the same year came to work in Shanghai Research Institute of Acupuncture and Meridian up to today, used to be a director of the Immunology Research Room of Shanghai Research Institute of Acupuncture and Meridian, and a consultant of Grade 3 Laboratory of Acupuncture Immunology of State Administration of Traditional Chinese Medicine, and is a vice chairwoman and deputy secretary general of Shanghai Professional Committee for the Respiratory Diseases of Integrated Chinese and Western Medicine. Zhao Cui-ying has been engaged in the clinical, research and teaching work in the field of immune-related diseases treated by acupuncture and moxibustion for over 40 years, with abundant and distinguished experience, has undertaken over 10 state and provincial subjects and offered many important viewpoints in the research of acupuncture treatment of immune-related diseases, and has obtained several scientific and technological achievements, and published totally 80 academic papers, and has been honored with 10 awards, including 6 provincial awards. As a tutor or instructor, she has trained 7 doctoral students and 16 master students.
基金We appreciate the financial support from the Major Science and technology Program of Heilongjiang(2020ZX08B02)National Natural Science Foundation of China(32072258)the National Key Research and Development Program of China(2021YFD2100902-3),Central financial support for the development of local colleges and Universities.
文摘SGDWSDIGGR(S-10-R)is a kind of antioxidant peptide derived from broken rice.In the present study,the effects of S-10-R against H_(2)O_(2)-induced cell damage and premature senescence in 2BS cells were determined.The results indicated that S-10-R could improve cell viability and mitochondrial membrane potential at least 1.06 and 1.12 folds compared with senescence cells.Moreover,S-10-R inhibitedβ-galactosidase,and DNA damage alleviates the effect on 2BS senescence cells due to scavenging ROS.The apoptosis rate can be reduced by 80.58%.Furthermore,S-10-R could inhibit PI3K and Akt phosphorylation,downregulate Bax and caspases-3 expression,and inhibit premature senescence through activating PI3K/Akt related intrinsic apoptotic signaling pathways and suppressing JNK/Bcl-2 related mitochondria-dependent apoptosis.The present study confirmed the feasibility of antioxidants as anti-aging drugs,as well as provided a substantial basis for future application of S-10-R as a functional ingredient against oxidative stress-induced premature aging.
