Bone marrow-derived mesenchymal stem cells （BMSCs） have been shown to promote the regeneration of injured peripheral nerves. Pulsed electromagnetic field （PEMF） reportedly promotes the proliferation and neuronal d...Bone marrow-derived mesenchymal stem cells （BMSCs） have been shown to promote the regeneration of injured peripheral nerves. Pulsed electromagnetic field （PEMF） reportedly promotes the proliferation and neuronal differentiation of BMSCs. Low-frequency PEMF can induce the neuronal differentiation of BMSCs in the absence of nerve growth factors. This study was designed to investigate the effects of low-frequency PEMF pretreatment on the proliferation and function of BMSCs and the effects of low-frequency PEMF pre-treated BMSCs on the regeneration of injured peripheral nerve using in vitro and in vivo experiments.In in vitro experiments, quantitative DNA analysis was performed to determine the proliferation of BMSCs, and reverse transcription-polymerase chain reaction was performed to detect S100 （Schwann cell marker）, glial fibrillary acidic protein （astrocyte marker）, and brain-derived neurotrophic factor and nerve growth factor （neurotrophic factors） mRNA expression. In the in vivo experiments, rat models of crush-injured mental nerve established using clamp method were randomly injected with low-frequency PEMF pretreated BMSCs, unpretreated BMSCs or PBS at the injury site （1 × 106 cells）. DiI-labeled BMSCs injected at the injury site were counted under the fluorescence microscope to determine cell survival. One or two weeks after cell injection, functional recovery of the injured nerve was assessed using the sensory test with von Frey filaments. Two weeks after cell injection, axonal regeneration was evaluated using histomorphometric analysis and retrograde labeling of trigeminal ganglion neurons. In vitro experiment results revealed that low-frequency PEMF pretreated BMSCs proliferated faster and had greater mRNA expression of growth factors than unpretreated BMSCs. In vivo experiment results revealed that compared with injection of unpretreated BMSCs, injection of low-frequency PEMF pretreated BMSCs led to higher myelinated axon count and axon density and more DiI-labeled neurons in th展开更多
Objective:To observe the effect of electroacupuncture combined with low-frequency pulsed electromagnetic field on bone metabolism in patients with spinal cord injury(SCI).Methods:The 60 SCI patients who met the inclus...Objective:To observe the effect of electroacupuncture combined with low-frequency pulsed electromagnetic field on bone metabolism in patients with spinal cord injury(SCI).Methods:The 60 SCI patients who met the inclusion criteria were randomly divided into 2 groups by means of a random number table.In the control group,30 patients were treated with osteoporosis treatment apparatus.In the observation group,30 patients were treated with osteoporosis treatment apparatus and electroacupuncture at the foot-shaoyangacupoints [Yánglíngquán(阳陵泉GB 34) and Xuánzhōng(悬钟GB 39) of both sides].The treatment was applied once a day,5 days a week,for 8 weeks.Before and after treatment,the changes of indexes in the two groups,including the bone mineral density(BMD),bone gla-containing protein(BGP),serum alkaline phosphatase(ALP),procollagen type I C-peptide(PICP),and 25-hydroxy-vitamin D [25(OH)D],were observed for comparison.Results:After treatment,in the two groups,the BMD and 25(OH)D indexes of the femoral neck,greater trochanter,and Ward’s triangle region were not significantly changed in comparing with those before treatment(P>0.05),while the BGP,ALP,and PICP indexes were significantly higher than those before treatment(P<0.05).After treatment,in the observation group,the BGP and ALP indexes were significantly increased in comparing with those of the control group,and the difference was statistically significant(P<0.05),whilst the BMD,PICP,and 25(OH)D indexes of the femoral neck,greater trochanter,and wards triangle region,were not significantly different in comparing with the control group(P>0.05).Conclusion:The osteoporosis treatment apparatus can improve bone metabolism in SCI patients,and the curative effect can be enhanced when combined with electroacupuncture at foot-shaoyangacupoints.展开更多
基金supported by a grant of the Korea Health Technology R & D Project through the Korea Health Industry Development Institute(KHIDI)funded by the Ministry of Health&Welfare,Republic of Korea(grant number:HI15C1535)
文摘Bone marrow-derived mesenchymal stem cells （BMSCs） have been shown to promote the regeneration of injured peripheral nerves. Pulsed electromagnetic field （PEMF） reportedly promotes the proliferation and neuronal differentiation of BMSCs. Low-frequency PEMF can induce the neuronal differentiation of BMSCs in the absence of nerve growth factors. This study was designed to investigate the effects of low-frequency PEMF pretreatment on the proliferation and function of BMSCs and the effects of low-frequency PEMF pre-treated BMSCs on the regeneration of injured peripheral nerve using in vitro and in vivo experiments.In in vitro experiments, quantitative DNA analysis was performed to determine the proliferation of BMSCs, and reverse transcription-polymerase chain reaction was performed to detect S100 （Schwann cell marker）, glial fibrillary acidic protein （astrocyte marker）, and brain-derived neurotrophic factor and nerve growth factor （neurotrophic factors） mRNA expression. In the in vivo experiments, rat models of crush-injured mental nerve established using clamp method were randomly injected with low-frequency PEMF pretreated BMSCs, unpretreated BMSCs or PBS at the injury site （1 × 106 cells）. DiI-labeled BMSCs injected at the injury site were counted under the fluorescence microscope to determine cell survival. One or two weeks after cell injection, functional recovery of the injured nerve was assessed using the sensory test with von Frey filaments. Two weeks after cell injection, axonal regeneration was evaluated using histomorphometric analysis and retrograde labeling of trigeminal ganglion neurons. In vitro experiment results revealed that low-frequency PEMF pretreated BMSCs proliferated faster and had greater mRNA expression of growth factors than unpretreated BMSCs. In vivo experiment results revealed that compared with injection of unpretreated BMSCs, injection of low-frequency PEMF pretreated BMSCs led to higher myelinated axon count and axon density and more DiI-labeled neurons in th
基金Supported by Special fund project of science and technology innovation,Wanzhou district,Chongqing:wzstc-042017089。
文摘Objective:To observe the effect of electroacupuncture combined with low-frequency pulsed electromagnetic field on bone metabolism in patients with spinal cord injury(SCI).Methods:The 60 SCI patients who met the inclusion criteria were randomly divided into 2 groups by means of a random number table.In the control group,30 patients were treated with osteoporosis treatment apparatus.In the observation group,30 patients were treated with osteoporosis treatment apparatus and electroacupuncture at the foot-shaoyangacupoints [Yánglíngquán(阳陵泉GB 34) and Xuánzhōng(悬钟GB 39) of both sides].The treatment was applied once a day,5 days a week,for 8 weeks.Before and after treatment,the changes of indexes in the two groups,including the bone mineral density(BMD),bone gla-containing protein(BGP),serum alkaline phosphatase(ALP),procollagen type I C-peptide(PICP),and 25-hydroxy-vitamin D [25(OH)D],were observed for comparison.Results:After treatment,in the two groups,the BMD and 25(OH)D indexes of the femoral neck,greater trochanter,and Ward’s triangle region were not significantly changed in comparing with those before treatment(P>0.05),while the BGP,ALP,and PICP indexes were significantly higher than those before treatment(P<0.05).After treatment,in the observation group,the BGP and ALP indexes were significantly increased in comparing with those of the control group,and the difference was statistically significant(P<0.05),whilst the BMD,PICP,and 25(OH)D indexes of the femoral neck,greater trochanter,and wards triangle region,were not significantly different in comparing with the control group(P>0.05).Conclusion:The osteoporosis treatment apparatus can improve bone metabolism in SCI patients,and the curative effect can be enhanced when combined with electroacupuncture at foot-shaoyangacupoints.
