Purpose:To investigate the mechanism of the termination of ocular dominance column plasticity by electrophysiologic analysis and 2-dimensional electrophoresis-mass spectrography(2-DE/MS).Methods:The changes in ocular ...Purpose:To investigate the mechanism of the termination of ocular dominance column plasticity by electrophysiologic analysis and 2-dimensional electrophoresis-mass spectrography(2-DE/MS).Methods:The changes in ocular dominance columns following monocular deprivation were electrophysiologically detected in 22-day-old,100-day-old and chondroitinase-perfused 100-day-old rats.Total protein of grey matter of the primary visual cortex was extracted and studied by 2-DE/MS from the three groups of rats.Results:Monocular deprivation may lead to shifts in ocular dominance columns in 22-day-old and chondroitinase-perfused 100-day-old rats,but not in 100-day-old rats.Four protein spots present in grey matter of the primary visual cortex in 100-day-old,but not in that of 22-day-old and chondroitinase-perfused rats,and mass spectrography identified two of these proteins.Conclusions:The electrophysiologic results show that ocular dominance column plasticity presents in 22-day-old rats,ends up in 100-day-old rats and restored in chondroitinase-perfused 100-day-old rats.2-DE/MS results show that phosphatidylethanolamine binding protein and glial fibrillary acidic protein delta may be associated with the termination of ocular dominance column plasticity in the rat,but need more evidence to confirm it.展开更多
An Otsuka Long-Evans Tokushima Fatty(OLETF)rat provides a useful model for studies to develop corneal wound healing drugs for use in diabetic keratopathy resulting from type 2 diabetes mellitus.We investigated the eff...An Otsuka Long-Evans Tokushima Fatty(OLETF)rat provides a useful model for studies to develop corneal wound healing drugs for use in diabetic keratopathy resulting from type 2 diabetes mellitus.We investigated the effects of sericin on corneal wound healing in OLETF rats.Corneal wounds were prepared by removal of the corneal epithelium and documented using a TRC-50X.Sericin was instilled into the eyes of rats five times a day following corneal abrasion.The plasma levels of glucose,triglycerides,cholesterol and insulin in 38 wk old OLETF rats were significantly higher than in normal control rats(LETO rats),and the rate of corneal wound healing in OLETF rats was slower than in normal rat,probably due to the suppression of cell migration and proliferation caused by high plasma glucose levels.The corneal wounds of OLETF rats instilled with saline showed almost complete healing 72h after corneal epithelial abrasion.On the other hand,the instillation of sericin has a potent effect in promoting wound healing and wound size reduction in OLETF rats and the wounds showed almost complete healing at 48 h after abrasion.The sericin may be an effective and safe drug to promote corneal wound healing in diabetic keratopathy.展开更多
文摘Purpose:To investigate the mechanism of the termination of ocular dominance column plasticity by electrophysiologic analysis and 2-dimensional electrophoresis-mass spectrography(2-DE/MS).Methods:The changes in ocular dominance columns following monocular deprivation were electrophysiologically detected in 22-day-old,100-day-old and chondroitinase-perfused 100-day-old rats.Total protein of grey matter of the primary visual cortex was extracted and studied by 2-DE/MS from the three groups of rats.Results:Monocular deprivation may lead to shifts in ocular dominance columns in 22-day-old and chondroitinase-perfused 100-day-old rats,but not in 100-day-old rats.Four protein spots present in grey matter of the primary visual cortex in 100-day-old,but not in that of 22-day-old and chondroitinase-perfused rats,and mass spectrography identified two of these proteins.Conclusions:The electrophysiologic results show that ocular dominance column plasticity presents in 22-day-old rats,ends up in 100-day-old rats and restored in chondroitinase-perfused 100-day-old rats.2-DE/MS results show that phosphatidylethanolamine binding protein and glial fibrillary acidic protein delta may be associated with the termination of ocular dominance column plasticity in the rat,but need more evidence to confirm it.
文摘An Otsuka Long-Evans Tokushima Fatty(OLETF)rat provides a useful model for studies to develop corneal wound healing drugs for use in diabetic keratopathy resulting from type 2 diabetes mellitus.We investigated the effects of sericin on corneal wound healing in OLETF rats.Corneal wounds were prepared by removal of the corneal epithelium and documented using a TRC-50X.Sericin was instilled into the eyes of rats five times a day following corneal abrasion.The plasma levels of glucose,triglycerides,cholesterol and insulin in 38 wk old OLETF rats were significantly higher than in normal control rats(LETO rats),and the rate of corneal wound healing in OLETF rats was slower than in normal rat,probably due to the suppression of cell migration and proliferation caused by high plasma glucose levels.The corneal wounds of OLETF rats instilled with saline showed almost complete healing 72h after corneal epithelial abrasion.On the other hand,the instillation of sericin has a potent effect in promoting wound healing and wound size reduction in OLETF rats and the wounds showed almost complete healing at 48 h after abrasion.The sericin may be an effective and safe drug to promote corneal wound healing in diabetic keratopathy.
