The influences of an Fe cap layer on the structural and magnetic properties of FePt/Fe bi-layers are investigated. Compared with single FePt alloy films, a thin Fe layer can affect the crystalline orientation and impr...The influences of an Fe cap layer on the structural and magnetic properties of FePt/Fe bi-layers are investigated. Compared with single FePt alloy films, a thin Fe layer can affect the crystalline orientation and improve the chemical ordering of L10 FePt films. Moreover, the coercivity increases when a thin Fe layer covers the FePt layer. Beyond a critical thickness, however, the Fe cover layer quickens the magnetization reversal of Fe49Pt51/Fe bi-layers by their exchange coupling.展开更多
为建立利用重组李氏杆菌溶血素(LLO)检测单核细胞增生李氏杆菌的ELISA方法,试验首先优化了重组李氏杆菌溶血素的表达条件,获得纯度较高的包涵体之后,用亲和层析法纯化重组李氏杆菌溶血素蛋白,并用纯化蛋白免疫试验兔获得诊断抗体,然后...为建立利用重组李氏杆菌溶血素(LLO)检测单核细胞增生李氏杆菌的ELISA方法,试验首先优化了重组李氏杆菌溶血素的表达条件,获得纯度较高的包涵体之后,用亲和层析法纯化重组李氏杆菌溶血素蛋白,并用纯化蛋白免疫试验兔获得诊断抗体,然后建立阻断ELISA检测单核细胞增生李氏杆菌的方法,并分析最低检测菌数。结果表明:在振摇培养条件下,当IPTG浓度为0.6 mmol/L、诱导温度为37℃、菌液OD600值为0.25时开始诱导,诱导时间为150 m in,重组李氏杆菌溶血素的表达量最大;纯化蛋白浓度为480μg/mL,具有免疫活性。说明建立的检测方法特异性好,最低检测菌数为4.8×106个/mL。提示利用重组李氏杆菌溶血素检测单核细胞增生李氏杆菌的ELISA方法是可行的,具有潜在的应用价值。展开更多
基金Project supported by the National Natural Science Foundation of China (Grant Nos 60490290,60678008 and 50771032)the National Basic Research Program of China (Grant No 2009CB929201)the Science Foundation of the Science and Technology Committee of Shanghai (Grant No 06DJ14007)
文摘The influences of an Fe cap layer on the structural and magnetic properties of FePt/Fe bi-layers are investigated. Compared with single FePt alloy films, a thin Fe layer can affect the crystalline orientation and improve the chemical ordering of L10 FePt films. Moreover, the coercivity increases when a thin Fe layer covers the FePt layer. Beyond a critical thickness, however, the Fe cover layer quickens the magnetization reversal of Fe49Pt51/Fe bi-layers by their exchange coupling.
文摘为建立利用重组李氏杆菌溶血素(LLO)检测单核细胞增生李氏杆菌的ELISA方法,试验首先优化了重组李氏杆菌溶血素的表达条件,获得纯度较高的包涵体之后,用亲和层析法纯化重组李氏杆菌溶血素蛋白,并用纯化蛋白免疫试验兔获得诊断抗体,然后建立阻断ELISA检测单核细胞增生李氏杆菌的方法,并分析最低检测菌数。结果表明:在振摇培养条件下,当IPTG浓度为0.6 mmol/L、诱导温度为37℃、菌液OD600值为0.25时开始诱导,诱导时间为150 m in,重组李氏杆菌溶血素的表达量最大;纯化蛋白浓度为480μg/mL,具有免疫活性。说明建立的检测方法特异性好,最低检测菌数为4.8×106个/mL。提示利用重组李氏杆菌溶血素检测单核细胞增生李氏杆菌的ELISA方法是可行的,具有潜在的应用价值。
