AIM： To investigate the possible role of c-erbB-2 and glutathione S-transferase （GST-Pi） in primary hepatocellular carcinogenesis and the relationship between liver hyperplastic nodule （LHN）, liver cirrhosis （LC...AIM： To investigate the possible role of c-erbB-2 and glutathione S-transferase （GST-Pi） in primary hepatocellular carcinogenesis and the relationship between liver hyperplastic nodule （LHN）, liver cirrhosis （LC）, and hepatocellular carcinoma （HCC）.METHODS： The expression of c-erbB-2 and GST-Pi was detected immunohistochemically in 42 tissue specimens of HCC and 77 specimens of its adjacent tissue.RESULTS： The positive expression of c-erbB-2 in LHN （28.6%） was significantly higher than that in LC （0%） （P = 0.032〈0.05）, but no significant difference was seen between HCC and LHN or LC （P〉0.05, X^2 = 0.002, 3.447）.The positive expression of GST-Pi in HCC （89.6%） or LHN （72.2%） was significantly higher than that in LC（22.9%, P〈0.001, ;X^2= 49.91, 16.96）. There was a significant difference between HCC and LHN （P〈0.05,X^2= 6.353）.CONCLUSION： The c-erbB-2 expression is an early event in the pathogenesis of HCC. GST-Pi may be a marker enzyme for immunohistochemical detection of human HCC and its preneoplastic lesions. LHN seems to be a preneoplastic lesion related to hepatocarcinogenesis.展开更多
Objective: To investigate the mechanism of inflammatory-mediated toll-like receptor 4(TLR4)-p38 mitogen-activated protein kinase(p38 MAPK) pathway in Kupffer cells(KCs) of non-alcoholic steatohepatitis(NASH) rats and ...Objective: To investigate the mechanism of inflammatory-mediated toll-like receptor 4(TLR4)-p38 mitogen-activated protein kinase(p38 MAPK) pathway in Kupffer cells(KCs) of non-alcoholic steatohepatitis(NASH) rats and the intervention effect of soothing Gan(Liver) and invigorating Pi(Spleen) recipes on this pathway. Methods: After 1 week of acclimatization, 120 Sprague-Dawley male rats were randomly divided into 8 groups using a random number table(n=15 per group): normal group, model group, low-dose Chaihu Shugan Powder(柴胡疏肝散, CHSG) group(3.2 g/kg), high-dose CHSG group(9.6 g/kg), low-dose Shenling Baizhu Powder(参苓白术散, SLBZ) group(10 g/kg), high-dose SLBZ(30 g/kg) group, and low-and highdose integrated recipe(L-IR, H-IR) groups. All rats in the model and treatment groups were fed with a high-fat diet(HFD). The treatments were administrated by gastrogavage once daily and lasted for 26 weeks. The liver tissues were detected with hematoxylin-eosin(HE) and oil red O staining. Levels of liver lipids, serum lipids and transaminases were measured. KCs were isolated from the livers of rats to evaluate the mRNA expressions of TLR4 and p38 MAPK by real-time fluorescence quantitative polymerase chain reaction, and proteins expressions of TLR4, p-p38 MAPK and p38 MAPK by Western blot. Levels of inflammatory cytokines including tumor necrosis factor α(TNF-α), interleukin(IL)-1 and IL-6 in KCs were measured by enzyme-linked immunosorbent assay. Results: After 26 weeks of HFD feeding, HE and oil red O staining showed that the NASH model rats successfully reproduced typical pathogenesis and histopathological features. Compared with the normal group, the model group exhibited significant increases in body weight, liver weight, liver index, serum levels of total cholesterol(TC), triglyceride(TG), low-density lipoprotein cholesterol, and aspartate aminotransferase as well as TC and TG levels in liver tissues, and significant decrease in serum level of high-density lipoprotein cholesterol(P<0.05 or P<0.01), whi展开更多
基金Supported by the Scientific Research Foundation of Shandong Provincial Education Committee (J94, K26)
文摘AIM： To investigate the possible role of c-erbB-2 and glutathione S-transferase （GST-Pi） in primary hepatocellular carcinogenesis and the relationship between liver hyperplastic nodule （LHN）, liver cirrhosis （LC）, and hepatocellular carcinoma （HCC）.METHODS： The expression of c-erbB-2 and GST-Pi was detected immunohistochemically in 42 tissue specimens of HCC and 77 specimens of its adjacent tissue.RESULTS： The positive expression of c-erbB-2 in LHN （28.6%） was significantly higher than that in LC （0%） （P = 0.032〈0.05）, but no significant difference was seen between HCC and LHN or LC （P〉0.05, X^2 = 0.002, 3.447）.The positive expression of GST-Pi in HCC （89.6%） or LHN （72.2%） was significantly higher than that in LC（22.9%, P〈0.001, ;X^2= 49.91, 16.96）. There was a significant difference between HCC and LHN （P〈0.05,X^2= 6.353）.CONCLUSION： The c-erbB-2 expression is an early event in the pathogenesis of HCC. GST-Pi may be a marker enzyme for immunohistochemical detection of human HCC and its preneoplastic lesions. LHN seems to be a preneoplastic lesion related to hepatocarcinogenesis.
基金Supported by the National Natural Science Foundation of China(No.30973694)
文摘Objective: To investigate the mechanism of inflammatory-mediated toll-like receptor 4(TLR4)-p38 mitogen-activated protein kinase(p38 MAPK) pathway in Kupffer cells(KCs) of non-alcoholic steatohepatitis(NASH) rats and the intervention effect of soothing Gan(Liver) and invigorating Pi(Spleen) recipes on this pathway. Methods: After 1 week of acclimatization, 120 Sprague-Dawley male rats were randomly divided into 8 groups using a random number table(n=15 per group): normal group, model group, low-dose Chaihu Shugan Powder(柴胡疏肝散, CHSG) group(3.2 g/kg), high-dose CHSG group(9.6 g/kg), low-dose Shenling Baizhu Powder(参苓白术散, SLBZ) group(10 g/kg), high-dose SLBZ(30 g/kg) group, and low-and highdose integrated recipe(L-IR, H-IR) groups. All rats in the model and treatment groups were fed with a high-fat diet(HFD). The treatments were administrated by gastrogavage once daily and lasted for 26 weeks. The liver tissues were detected with hematoxylin-eosin(HE) and oil red O staining. Levels of liver lipids, serum lipids and transaminases were measured. KCs were isolated from the livers of rats to evaluate the mRNA expressions of TLR4 and p38 MAPK by real-time fluorescence quantitative polymerase chain reaction, and proteins expressions of TLR4, p-p38 MAPK and p38 MAPK by Western blot. Levels of inflammatory cytokines including tumor necrosis factor α(TNF-α), interleukin(IL)-1 and IL-6 in KCs were measured by enzyme-linked immunosorbent assay. Results: After 26 weeks of HFD feeding, HE and oil red O staining showed that the NASH model rats successfully reproduced typical pathogenesis and histopathological features. Compared with the normal group, the model group exhibited significant increases in body weight, liver weight, liver index, serum levels of total cholesterol(TC), triglyceride(TG), low-density lipoprotein cholesterol, and aspartate aminotransferase as well as TC and TG levels in liver tissues, and significant decrease in serum level of high-density lipoprotein cholesterol(P<0.05 or P<0.01), whi
