Background：Tuberculosis （TB） is a chronic wasting inflammatory disease characterized by multisystem involvement,which can cause metabolic derangements in afflicted patients.Metabolic signatures have been exploited ...Background：Tuberculosis （TB） is a chronic wasting inflammatory disease characterized by multisystem involvement,which can cause metabolic derangements in afflicted patients.Metabolic signatures have been exploited in the study of several diseases.However,the serum that is successfully used in TB diagnosis on the basis of metabolic profiling is not by much.Methods：Orthogonal partial least-squares discriminant analysis was capable of distinguishing TB patients from both healthy subjects and patients with conditions other than TB.Therefore,TB-specific metabolic profiling was established.Clusters of potential biomarkers for differentiating TB active from non-TB diseases were identified using Mann-Whitney U-test.Multiple logistic regression analysis of metabolites was calculated to determine the suitable biomarker group that allows the efficient differentiation of patients with TB active from the control subjects.Results：From among 271 participants,12 metabolites were found to contribute to the distinction between the TB active group and the control groups.These metabolites were mainly involved in the metabolic pathways of the following three biomolecules：Fatty acids,amino acids,and lipids.The receiver operating characteristic curves of3D,7D,and 11D-phytanic acid,behenic acid,and threoninyl-γ-glutamate exhibited excellent efficiency with area under the curve （AUC） values of 0.904 （95% confidence interval [CI]：0.863-0.944）,0.93 （95% CI：0.893-0.966）,and 0.964 （95% CI：0.941-0.988）,respectively.The largest and smallest resulting AUCs were 0.964 and 0.720,indicating that these biomarkers may be involved in the disease mechanisms.The combination of lysophosphatidylcholine （18∶0）,behenic acid,threoninyl-γ-glutamate,and presqualene diphosphate was used to represent the most suitable biomarker group for the differentiation of patients with TB active from the control subjects,with an AUC value of 0.991.Conclusion：The metabolic analysis results identified new serum biomarkers that can 展开更多
目的明确茄子中施用矮壮素的作用,分析矮壮素在茄子中的残留行为和评估其膳食暴露风险。方法开展矮壮素在茄子中的规范残留试验,茄子样品经乙酸-乙腈提取,超高效液相色谱-串联质谱法(ultra performance liquid chromatography tandem ma...目的明确茄子中施用矮壮素的作用,分析矮壮素在茄子中的残留行为和评估其膳食暴露风险。方法开展矮壮素在茄子中的规范残留试验,茄子样品经乙酸-乙腈提取,超高效液相色谱-串联质谱法(ultra performance liquid chromatography tandem mass spectrometry,UPLC-MS/MS)检测,分析矮壮素在茄子中的残留行为,监测矮壮素对茄子生长和品质的影响,用农药残留联席会议的方法对茄子中矮壮素残留带来的膳食摄入风险进行评估。结果矮壮素在0.002~0.5 mg/L的范围内线性关系良好,在茄子中的检出限和定量限分别为0.0005和0.002 mg/kg。在0.002、0.01和0.02 mg/kg添加水平下,回收率在86.7%~102.3%,相对标准偏差为6.1%~8.2%。茄子中矮壮素残留对我国各类人群的膳食摄入暴露量为0.51~1.4μg/(kg bw·d),占ARf D的1.02%~2.8%。矮壮素应用在茄子中的推荐使用剂量为9 g ai/ha浓度喷施2次,茄子中矮壮素残留带来的膳食摄入风险极低。结论本研究为茄子中矮壮素的合理使用、科学监管及最大残留限量标准的制定提供依据。展开更多
果蝇样品经固相萃取后,采用高效液相色谱-串联质谱法进行样品分析,检测条件用Endeavorsil^(TM)C_(18)色谱柱(100 mm×2.1 mm,1.8μm),以0.1%甲酸水-乙腈为流动相,流速为0.3 m L/min,柱温40℃;采用电喷雾离子源负离子多重反应监测模...果蝇样品经固相萃取后,采用高效液相色谱-串联质谱法进行样品分析,检测条件用Endeavorsil^(TM)C_(18)色谱柱(100 mm×2.1 mm,1.8μm),以0.1%甲酸水-乙腈为流动相,流速为0.3 m L/min,柱温40℃;采用电喷雾离子源负离子多重反应监测模式,内标标准工作曲线定量,建立了果蝇组织中15种多不饱和脂肪酸代谢产物——类二十烷酸的测定方法。结果显示,在2.5~200 ng/m L范围内呈良好的线性关系(r=0.991),回收率范围在89.3%~108.9%,日内精密度相对标准偏差(relative standard deviation,RSD)在1.2%~12.4%,日间精密度RSD在1.0%~15.0%,检出限0.1~2.6 ng/g,定量限0.3~8.7 ng/g。所建方法简便、准确、选择性高,为研究模式生物果蝇对多不饱和脂肪酸的代谢特性提供了科学依据。展开更多
文摘Background：Tuberculosis （TB） is a chronic wasting inflammatory disease characterized by multisystem involvement,which can cause metabolic derangements in afflicted patients.Metabolic signatures have been exploited in the study of several diseases.However,the serum that is successfully used in TB diagnosis on the basis of metabolic profiling is not by much.Methods：Orthogonal partial least-squares discriminant analysis was capable of distinguishing TB patients from both healthy subjects and patients with conditions other than TB.Therefore,TB-specific metabolic profiling was established.Clusters of potential biomarkers for differentiating TB active from non-TB diseases were identified using Mann-Whitney U-test.Multiple logistic regression analysis of metabolites was calculated to determine the suitable biomarker group that allows the efficient differentiation of patients with TB active from the control subjects.Results：From among 271 participants,12 metabolites were found to contribute to the distinction between the TB active group and the control groups.These metabolites were mainly involved in the metabolic pathways of the following three biomolecules：Fatty acids,amino acids,and lipids.The receiver operating characteristic curves of3D,7D,and 11D-phytanic acid,behenic acid,and threoninyl-γ-glutamate exhibited excellent efficiency with area under the curve （AUC） values of 0.904 （95% confidence interval [CI]：0.863-0.944）,0.93 （95% CI：0.893-0.966）,and 0.964 （95% CI：0.941-0.988）,respectively.The largest and smallest resulting AUCs were 0.964 and 0.720,indicating that these biomarkers may be involved in the disease mechanisms.The combination of lysophosphatidylcholine （18∶0）,behenic acid,threoninyl-γ-glutamate,and presqualene diphosphate was used to represent the most suitable biomarker group for the differentiation of patients with TB active from the control subjects,with an AUC value of 0.991.Conclusion：The metabolic analysis results identified new serum biomarkers that can
文摘目的:为电针联合雷公藤甲素(triptolide,TPL)治疗类风湿关节炎(rheumatoid arthritis,RA),同时减轻TPL所致的肝损提供增效减毒的实验依据。方法:RA模型采用弗氏完全佐剂(freund’s complete adjuvant,CFA)建立,运用液相色谱-质谱联用技术(liquid chromatography-mass spectrometry,LC-MS)检测大鼠血浆TPL浓度,试剂盒测定肝匀浆中谷氨酸-丙酮酸转氨酶(ALT)、天冬氨酸氨基转移酶(AST)含量及超氧化物歧化酶(superoxide dismutase,SOD)、丙二醛(malondialdehyde,MDA)活性。结果:针药组大鼠足趾肿胀改善情况最好,药物组次之,再者为电针组;在同一时刻电针刺"足三里"后的血药浓度高于单独口服TPL的浓度,TPL的药动学参数:达峰时间(T_(max))提前,峰浓度(C_(max))及药时曲线下面积(area under the curve,AUC)提高;针药组ALT、AST含量及MDA活性均较药物组降低,SOD活性则升高。结论:电针"足三里"可以减轻RA大鼠足趾肿胀,提高TPL的血药浓度,进而提高其生物利用度,同时还可减轻TPL对RA大鼠肝组织的损伤,提高其抗氧化应激能力。
文摘目的明确茄子中施用矮壮素的作用,分析矮壮素在茄子中的残留行为和评估其膳食暴露风险。方法开展矮壮素在茄子中的规范残留试验,茄子样品经乙酸-乙腈提取,超高效液相色谱-串联质谱法(ultra performance liquid chromatography tandem mass spectrometry,UPLC-MS/MS)检测,分析矮壮素在茄子中的残留行为,监测矮壮素对茄子生长和品质的影响,用农药残留联席会议的方法对茄子中矮壮素残留带来的膳食摄入风险进行评估。结果矮壮素在0.002~0.5 mg/L的范围内线性关系良好,在茄子中的检出限和定量限分别为0.0005和0.002 mg/kg。在0.002、0.01和0.02 mg/kg添加水平下,回收率在86.7%~102.3%,相对标准偏差为6.1%~8.2%。茄子中矮壮素残留对我国各类人群的膳食摄入暴露量为0.51~1.4μg/(kg bw·d),占ARf D的1.02%~2.8%。矮壮素应用在茄子中的推荐使用剂量为9 g ai/ha浓度喷施2次,茄子中矮壮素残留带来的膳食摄入风险极低。结论本研究为茄子中矮壮素的合理使用、科学监管及最大残留限量标准的制定提供依据。
