A rapid method to obtain large amount of VLDL and LDL by ultracentrifugation is described. The mixture of VLDL and LDL was isolated and concentrated from plasma by an ultracentrifugation at 265 000 g for 2h. VLDL and ...A rapid method to obtain large amount of VLDL and LDL by ultracentrifugation is described. The mixture of VLDL and LDL was isolated and concentrated from plasma by an ultracentrifugation at 265 000 g for 2h. VLDL and LDL were separated and purified by a further ultracentrifugation at 265 000g for 3h.This method combines the advantages of both sequential flotation ultracentri-fugation and density gradient ultracentrifugation. It can process a large volume of plasma in a short time. The purity of isolated VLDL and LDL was confirmed by the lipoprotein electrophoresis on agarose gel and PAGE and by the apolipoprotein electrophoresis on SDS-PAGE. This rapid.economical method is of great value in practical application.展开更多
缺血性心肌损伤的分子病理机制一直备受国际关注。该文应用C57BL/6品系的野生型(WT)小鼠、极低密度脂蛋白(Very Low Density Lipoprotein,VLDL)受体基因敲除(VLDLR-KO)小鼠、心脏特异性转基因(VLDLR-TG)小鼠,通过在体及离体实验研究VLD...缺血性心肌损伤的分子病理机制一直备受国际关注。该文应用C57BL/6品系的野生型(WT)小鼠、极低密度脂蛋白(Very Low Density Lipoprotein,VLDL)受体基因敲除(VLDLR-KO)小鼠、心脏特异性转基因(VLDLR-TG)小鼠,通过在体及离体实验研究VLDL受体对小鼠缺血性心肌损伤的影响,构建生物分子网络研究VLDL受体发挥生物学功能的关键分子及其关键信号通路。通过生物网络的功能模块计算,发现了VLDL受体→MAPKs、AKT、NF-κB信号通路→心肌损伤病理表型的因果关联性,阐明了VLDL受体影响缺血性心肌损伤的分子机制。展开更多
文摘A rapid method to obtain large amount of VLDL and LDL by ultracentrifugation is described. The mixture of VLDL and LDL was isolated and concentrated from plasma by an ultracentrifugation at 265 000 g for 2h. VLDL and LDL were separated and purified by a further ultracentrifugation at 265 000g for 3h.This method combines the advantages of both sequential flotation ultracentri-fugation and density gradient ultracentrifugation. It can process a large volume of plasma in a short time. The purity of isolated VLDL and LDL was confirmed by the lipoprotein electrophoresis on agarose gel and PAGE and by the apolipoprotein electrophoresis on SDS-PAGE. This rapid.economical method is of great value in practical application.
文摘缺血性心肌损伤的分子病理机制一直备受国际关注。该文应用C57BL/6品系的野生型(WT)小鼠、极低密度脂蛋白(Very Low Density Lipoprotein,VLDL)受体基因敲除(VLDLR-KO)小鼠、心脏特异性转基因(VLDLR-TG)小鼠,通过在体及离体实验研究VLDL受体对小鼠缺血性心肌损伤的影响,构建生物分子网络研究VLDL受体发挥生物学功能的关键分子及其关键信号通路。通过生物网络的功能模块计算,发现了VLDL受体→MAPKs、AKT、NF-κB信号通路→心肌损伤病理表型的因果关联性,阐明了VLDL受体影响缺血性心肌损伤的分子机制。
