从不同地区直链烷基苯磺酸钠(linear sodium alkylbenzenesulfonate,简称LAS)富集地带取回的污水样中分离纯化得到一株能高效降解直链烷基苯磺酸钠的菌株——可动苯基杆菌(phenylobaterium mobil GZ6)。该菌细胞为短杆状,大小为(0.5～0...从不同地区直链烷基苯磺酸钠(linear sodium alkylbenzenesulfonate,简称LAS)富集地带取回的污水样中分离纯化得到一株能高效降解直链烷基苯磺酸钠的菌株——可动苯基杆菌(phenylobaterium mobil GZ6)。该菌细胞为短杆状,大小为(0.5～0.8)μm×(1.0～2.0)μm,其生长pH为6.0～10.0,最适生长pH为7.0,生长温度为4～40℃,最适生长温度为28℃。本实验用温度为28℃,转速为170r/min的振荡摇床培养GZ6菌,并实时监测其对LAS的降解情况。由紫外分析图发现,在GZ6对LAS降解的第8h,出现吸光度变化的最大值点,取第8h的样品用HPLC法分离,并用MS法进一步佐证紫外吸收的分析结果,探讨了GZ6菌对LAS的降解机理。展开更多
High performance liquid chromatography (HPLC) has the advantages of rapid analysis and high sensitivity. In the present study a suitable analytical condition has been established for HPLC, and the LAS in modern sedime...High performance liquid chromatography (HPLC) has the advantages of rapid analysis and high sensitivity. In the present study a suitable analytical condition has been established for HPLC, and the LAS in modern sediments from core Zhu-9 at the Pearl River mouth has been determined by HPLC. The concentrations of C12-LAS homologues are the highest. The sedimentation flux of C12-LAS was correlated with the average flow of the Xijiang and Beijiang rivers: the higher the flow rate, the larger the C12-LAS sedimentation flux. This shows that the more rapidly the river flows, the shorter the LAS will stay in freshwater, the less the extent of biodegradation, the larger the C12-LAS sedimentation flux in the core Zhu-9.展开更多
文摘从不同地区直链烷基苯磺酸钠(linear sodium alkylbenzenesulfonate,简称LAS)富集地带取回的污水样中分离纯化得到一株能高效降解直链烷基苯磺酸钠的菌株——可动苯基杆菌(phenylobaterium mobil GZ6)。该菌细胞为短杆状,大小为(0.5～0.8)μm×(1.0～2.0)μm,其生长pH为6.0～10.0,最适生长pH为7.0,生长温度为4～40℃,最适生长温度为28℃。本实验用温度为28℃,转速为170r/min的振荡摇床培养GZ6菌,并实时监测其对LAS的降解情况。由紫外分析图发现,在GZ6对LAS降解的第8h,出现吸光度变化的最大值点,取第8h的样品用HPLC法分离,并用MS法进一步佐证紫外吸收的分析结果,探讨了GZ6菌对LAS的降解机理。
文摘High performance liquid chromatography (HPLC) has the advantages of rapid analysis and high sensitivity. In the present study a suitable analytical condition has been established for HPLC, and the LAS in modern sediments from core Zhu-9 at the Pearl River mouth has been determined by HPLC. The concentrations of C12-LAS homologues are the highest. The sedimentation flux of C12-LAS was correlated with the average flow of the Xijiang and Beijiang rivers: the higher the flow rate, the larger the C12-LAS sedimentation flux. This shows that the more rapidly the river flows, the shorter the LAS will stay in freshwater, the less the extent of biodegradation, the larger the C12-LAS sedimentation flux in the core Zhu-9.
