The quenching interaction of atomoxetine(ATX) with bovine serum albumin(BSA) was studied in vitro under optimal physiological condition(pH=7.4) by multi-spectroscopic techniques. The mechanism of ATX-BSA system was a ...The quenching interaction of atomoxetine(ATX) with bovine serum albumin(BSA) was studied in vitro under optimal physiological condition(pH=7.4) by multi-spectroscopic techniques. The mechanism of ATX-BSA system was a dynamic quenching process and was confirmed by the fluorescence spectra and lifetime measurements. The number of binding sites, binding constants and other binding characteristics were computed. Thermodynamic parameters ΔH^0 and ΔS^0 indicated that intermolecular hydrophobic forces predominantly stabilized the drug-protein system. The average binding distance between BSA and ATX was studied by F?rsters theory. UV-absorption, Fourier transform infrared spectroscopy(FT-IR), circular dichroism(CD), synchronous spectra and three-dimensional(3D) fluorescence spectral results revealed the changes in micro-environment of secondary structure of protein upon the interaction with ATX. Displacement of site probes and the effects of some common metal ions on the binding of ATX with BSA interaction were also studied.展开更多
An experimental measurement of the lifetime of 3d^(9) ^(2)D_(3/2) metastable level in Mo^(15+)is reported in this work.The Mo^(15+)ions are produced and trapped in an electron beam ion trap with a magnetic field of 0....An experimental measurement of the lifetime of 3d^(9) ^(2)D_(3/2) metastable level in Mo^(15+)is reported in this work.The Mo^(15+)ions are produced and trapped in an electron beam ion trap with a magnetic field of 0.65 T.The decay photons emitted from 3d^(9) ^(2)D_(3/2) level are subsequently recorded via a cooled photomultiplier tube.Through meticulous scrutiny of potential systematic uncertainties affecting the measurement outcomes,we have determined the lifetime of Mo^(15+)3d^(9)2D_(3/2)metastable level to be 2.83(22)ms.The experimental result provides a clear distinguishment from existing calculations based on various theoretical approaches.展开更多
设计和研制了一种简便的宇宙线μ子寿命测量的实验装置.该装置采用大面积闪烁体探测器,利用可编程逻辑阵列(field program mable gatearray,FPGA),并通过电子学脉冲计数法,实现对宇宙线μ子的衰变寿命测量.测量结果与文献中给出μ子寿...设计和研制了一种简便的宇宙线μ子寿命测量的实验装置.该装置采用大面积闪烁体探测器,利用可编程逻辑阵列(field program mable gatearray,FPGA),并通过电子学脉冲计数法,实现对宇宙线μ子的衰变寿命测量.测量结果与文献中给出μ子寿命精确值比较,误差小于3.5%.该实验原理及测量方法简单,测量条件要求不高,可用于大学物理实验教学.相关实验方法也可应用到其他一些粒子寿命及时间测量的实验中.展开更多
A great amount of work addressed methods for predicting the battery lifetime in wireless sensor systems. In spite of these efforts, the reported experimental results demonstrate that the duty-cycle current average met...A great amount of work addressed methods for predicting the battery lifetime in wireless sensor systems. In spite of these efforts, the reported experimental results demonstrate that the duty-cycle current average method, which is widely used to this aim, fails in accurately estimating the battery life time of most of the presented wireless sensor system applications. The aim of this paper is to experimentally assess the duty-cycle current average method in order to give more effective insight on the effectiveness of the method. An electronic metering system, based on a dedicated PCB, has been designed and developed to experimentally measure node current consumption profiles and charge extracted from the battery in two selected case studies. A battery lifetime measurement (during 30 days) has been carried out. Experimental results have been assessed and compared with estimations given by using the duty-cycle current average method. Based on the measurement results, we show that the assumptions on which the method is based do not hold in real operating cases. The rationality of the duty-cycle current average method needs reconsidering.展开更多
The high spin states of 129Ce have been populated via heavy-ion fusion evaporation reaction 96Mo (37Cl, 1p3n) 129Ce. The γ-γ coincidence and intensity balance used to measure the B(M1;I →I-1)/B(E2;I → I-2) (the pr...The high spin states of 129Ce have been populated via heavy-ion fusion evaporation reaction 96Mo (37Cl, 1p3n) 129Ce. The γ-γ coincidence and intensity balance used to measure the B(M1;I →I-1)/B(E2;I → I-2) (the probability ratio of the dipole and quadrupole transition) in ν7/2[523] rotational band of 129Ce. And the energy splitting (△e ’) has been got through the experimental Routhians. The lifetimes and quadrupole moments Qt have been extracted from the lineshape analyses using DSAM. The deformation of the ν7/2[523] rotational band of 129Ce was extracted from the Qt and moment of inertia JRR.展开更多
The lifetime of the first excited 2+ state in 18C was measured using an upgraded recoil shadow method to determine the electric quadrupole transition. The measured mean lifetime is 18.9±0.9(stat)±4.4(syst)ps...The lifetime of the first excited 2+ state in 18C was measured using an upgraded recoil shadow method to determine the electric quadrupole transition. The measured mean lifetime is 18.9±0.9(stat)±4.4(syst)ps,which corresponds to a B(E2; 2+1→0+gs) value of (4.3± 0.2±1.0) e2fm4,or about 1.5 Weisskopf units. The mean lifetime of the first 2+ state in 16C was remeasured to be about 18 ps,about four times shorter than the value reported previously. This discrepancy was explained by incorporating the γ-ray angular distribution measured in this work into the previous measurement. The observed transition strengths in 16,18C are hindered compared to the empirical transition strengths,indicating that the anomalous hindrance observed in 16C persists in 18C.展开更多
Precision measurement of the 4s2 S1/2-3d2 D5/2 clock transition based on 40Ca＋ ion at 729 nm is reported. A single 40Ca＋ ion is trapped and laser-cooled in a ring Paul trap, and the storage time for the ion is more ...Precision measurement of the 4s2 S1/2-3d2 D5/2 clock transition based on 40Ca＋ ion at 729 nm is reported. A single 40Ca＋ ion is trapped and laser-cooled in a ring Paul trap, and the storage time for the ion is more than one month. The linewidth of a 729 nm laser is reduced to about 1 Hz by locking to a super cavity for longer than one month uninterruptedly. The overall systematic uncertainty of the clock transition is evaluated to be better than 6.5 ×10^-16. The absolute frequency of the clock transition is measured at the 10^-15 level by using an optical frequency comb referenced to a hydrogen maser which is calibrated to the SI second through the global positioning system （GPS）, The frequency value is 411 042 129 776 393.0（1.6） Hz with the correction of the systematic shifts. In order to carry out the comparison of two 40Ca＋ optical frequency standards, another similar 40Ca＋ optical frequency standard is constructed. Two optical frequency standards exhibit stabilities of 1 × 10^-14 T-1/2 with 3 days of averaging. Moreover, two additional precision measurements based on the single trapped 40Ca＋ ion are carried out. One is the 3d2Ds/2 state lifetime measurement, and our result of 1174（10） ms agrees well with the results reported in [Phys. Rev. A 62 032503 （2000）] and [Phys. Rev. A 71 032504 （2005）]. The other one is magic wavelengths for the 4s2S1/2-3d2Ds/2 clock transition; λ ｜mj｜=1/2= 395.7992（7） nm and λ｜m｜=3/2 = 395.7990（7） nm are reported, and it is the first time that two magic wavelengths for the 40Ca＋ clock-transition have been reported.展开更多
Forster resonance energy transfer(FRET)is a widely used distance measurement method to illustrate protein conformational dynamics.The FRET method relies on the distance between donor and acceptor,as well as the labell...Forster resonance energy transfer(FRET)is a widely used distance measurement method to illustrate protein conformational dynamics.The FRET method relies on the distance between donor and acceptor,as well as the labelling efficiency,the size and the properties of the fluorophores.Here,we labelled a pair of small fluorophores and calculated the energy transferred efficiency through fluorescence lifetime analysis,which can provide more reliable distance measurement than intensity attenuation.The donor fluorophore,7-hydroxycoumarin-4-yl-ethylglycine(HC),was genetically incorporated into specific sites of PYL10,obtaining complete labelling efficiency.The acceptor fluorophore,Alexa488,was labelled through the disulfide bond,whose labelling efficiency was estimated through both absorption peaks and lifetime populations.Fluorescence lifetime and anisotropy analysis showed ABA-induced local conformation changes and dynamics of several HC incorporation sites of PYL10.The lifetime-based FRET distance measurement illustrated the conformation changes of PYL10 with or without ABA application,which is consistent with the previously reported crystal structures.展开更多
基金Karnatak University, Dharwad, India, for providing UGC-UPE fellowshipUGC, New Delhi for the award of BSR Faculty Fellowship (F No.18-1/2011) to Prof. S.T. Nandibewoor
文摘The quenching interaction of atomoxetine(ATX) with bovine serum albumin(BSA) was studied in vitro under optimal physiological condition(pH=7.4) by multi-spectroscopic techniques. The mechanism of ATX-BSA system was a dynamic quenching process and was confirmed by the fluorescence spectra and lifetime measurements. The number of binding sites, binding constants and other binding characteristics were computed. Thermodynamic parameters ΔH^0 and ΔS^0 indicated that intermolecular hydrophobic forces predominantly stabilized the drug-protein system. The average binding distance between BSA and ATX was studied by F?rsters theory. UV-absorption, Fourier transform infrared spectroscopy(FT-IR), circular dichroism(CD), synchronous spectra and three-dimensional(3D) fluorescence spectral results revealed the changes in micro-environment of secondary structure of protein upon the interaction with ATX. Displacement of site probes and the effects of some common metal ions on the binding of ATX with BSA interaction were also studied.
基金Project supported by the National Key R&D Program of China(Grant No.2022YFA1602500)the National Natural Science Foundation of China(Grant No.12274352)。
文摘An experimental measurement of the lifetime of 3d^(9) ^(2)D_(3/2) metastable level in Mo^(15+)is reported in this work.The Mo^(15+)ions are produced and trapped in an electron beam ion trap with a magnetic field of 0.65 T.The decay photons emitted from 3d^(9) ^(2)D_(3/2) level are subsequently recorded via a cooled photomultiplier tube.Through meticulous scrutiny of potential systematic uncertainties affecting the measurement outcomes,we have determined the lifetime of Mo^(15+)3d^(9)2D_(3/2)metastable level to be 2.83(22)ms.The experimental result provides a clear distinguishment from existing calculations based on various theoretical approaches.
文摘设计和研制了一种简便的宇宙线μ子寿命测量的实验装置.该装置采用大面积闪烁体探测器,利用可编程逻辑阵列(field program mable gatearray,FPGA),并通过电子学脉冲计数法,实现对宇宙线μ子的衰变寿命测量.测量结果与文献中给出μ子寿命精确值比较,误差小于3.5%.该实验原理及测量方法简单,测量条件要求不高,可用于大学物理实验教学.相关实验方法也可应用到其他一些粒子寿命及时间测量的实验中.
文摘A great amount of work addressed methods for predicting the battery lifetime in wireless sensor systems. In spite of these efforts, the reported experimental results demonstrate that the duty-cycle current average method, which is widely used to this aim, fails in accurately estimating the battery life time of most of the presented wireless sensor system applications. The aim of this paper is to experimentally assess the duty-cycle current average method in order to give more effective insight on the effectiveness of the method. An electronic metering system, based on a dedicated PCB, has been designed and developed to experimentally measure node current consumption profiles and charge extracted from the battery in two selected case studies. A battery lifetime measurement (during 30 days) has been carried out. Experimental results have been assessed and compared with estimations given by using the duty-cycle current average method. Based on the measurement results, we show that the assumptions on which the method is based do not hold in real operating cases. The rationality of the duty-cycle current average method needs reconsidering.
基金Supported by Major State Basic Research Development Program (2007CB815000)National Natural Science Foundation of China (10775184, 10575092, 10675171, 10375092, 10575133)
文摘The high spin states of 129Ce have been populated via heavy-ion fusion evaporation reaction 96Mo (37Cl, 1p3n) 129Ce. The γ-γ coincidence and intensity balance used to measure the B(M1;I →I-1)/B(E2;I → I-2) (the probability ratio of the dipole and quadrupole transition) in ν7/2[523] rotational band of 129Ce. And the energy splitting (△e ’) has been got through the experimental Routhians. The lifetimes and quadrupole moments Qt have been extracted from the lineshape analyses using DSAM. The deformation of the ν7/2[523] rotational band of 129Ce was extracted from the Qt and moment of inertia JRR.
基金Ministry of Education, Culture,Sports,Science and Technology of Japan(15204017,20740163)
文摘The lifetime of the first excited 2+ state in 18C was measured using an upgraded recoil shadow method to determine the electric quadrupole transition. The measured mean lifetime is 18.9±0.9(stat)±4.4(syst)ps,which corresponds to a B(E2; 2+1→0+gs) value of (4.3± 0.2±1.0) e2fm4,or about 1.5 Weisskopf units. The mean lifetime of the first 2+ state in 16C was remeasured to be about 18 ps,about four times shorter than the value reported previously. This discrepancy was explained by incorporating the γ-ray angular distribution measured in this work into the previous measurement. The observed transition strengths in 16,18C are hindered compared to the empirical transition strengths,indicating that the anomalous hindrance observed in 16C persists in 18C.
基金supported by the National Basic Research Program of China(Grant Nos.2012CB821301 and 2005CB724502)the National Natural Science Foundation of China(Grant Nos.11474318,91336211,and 11034009)Chinese Academy of Sciences
文摘Precision measurement of the 4s2 S1/2-3d2 D5/2 clock transition based on 40Ca＋ ion at 729 nm is reported. A single 40Ca＋ ion is trapped and laser-cooled in a ring Paul trap, and the storage time for the ion is more than one month. The linewidth of a 729 nm laser is reduced to about 1 Hz by locking to a super cavity for longer than one month uninterruptedly. The overall systematic uncertainty of the clock transition is evaluated to be better than 6.5 ×10^-16. The absolute frequency of the clock transition is measured at the 10^-15 level by using an optical frequency comb referenced to a hydrogen maser which is calibrated to the SI second through the global positioning system （GPS）, The frequency value is 411 042 129 776 393.0（1.6） Hz with the correction of the systematic shifts. In order to carry out the comparison of two 40Ca＋ optical frequency standards, another similar 40Ca＋ optical frequency standard is constructed. Two optical frequency standards exhibit stabilities of 1 × 10^-14 T-1/2 with 3 days of averaging. Moreover, two additional precision measurements based on the single trapped 40Ca＋ ion are carried out. One is the 3d2Ds/2 state lifetime measurement, and our result of 1174（10） ms agrees well with the results reported in [Phys. Rev. A 62 032503 （2000）] and [Phys. Rev. A 71 032504 （2005）]. The other one is magic wavelengths for the 4s2S1/2-3d2Ds/2 clock transition; λ ｜mj｜=1/2= 395.7992（7） nm and λ｜m｜=3/2 = 395.7990（7） nm are reported, and it is the first time that two magic wavelengths for the 40Ca＋ clock-transition have been reported.
基金supported by National Key R&D Program of China(Nos.2017YFA0505300,2016YFA0400900)the Instrument Developing Project of the Chinese Academy of Sciences(No.YZ201564)the National Natural Science Foundation of China(Nos.U1832181,31670776,31500611)
文摘Forster resonance energy transfer(FRET)is a widely used distance measurement method to illustrate protein conformational dynamics.The FRET method relies on the distance between donor and acceptor,as well as the labelling efficiency,the size and the properties of the fluorophores.Here,we labelled a pair of small fluorophores and calculated the energy transferred efficiency through fluorescence lifetime analysis,which can provide more reliable distance measurement than intensity attenuation.The donor fluorophore,7-hydroxycoumarin-4-yl-ethylglycine(HC),was genetically incorporated into specific sites of PYL10,obtaining complete labelling efficiency.The acceptor fluorophore,Alexa488,was labelled through the disulfide bond,whose labelling efficiency was estimated through both absorption peaks and lifetime populations.Fluorescence lifetime and anisotropy analysis showed ABA-induced local conformation changes and dynamics of several HC incorporation sites of PYL10.The lifetime-based FRET distance measurement illustrated the conformation changes of PYL10 with or without ABA application,which is consistent with the previously reported crystal structures.
