The present study identified the favorable environment conditions for Spermatogomial stem cells in vitro according to their unique biological properties. Three growth factors, stem cell factor (SCF), leukemia inhibito...The present study identified the favorable environment conditions for Spermatogomial stem cells in vitro according to their unique biological properties. Three growth factors, stem cell factor (SCF), leukemia inhibitory factor (LIF) and basic fibroblast growth factor (bFGF) were all found to independently contribute to the proliferation of mouse spermatogonial stem cell. The percentage of cell proliferation significantly enhanced by SCF at 30 ng/mL but decreased with heightening its combination after cultured 120 hours. The mice spermatogonial stem cells were significantly proliferated after 120 hours’ culture with 10 ng/mL and 20 ng/mL (P<0.01) of LIF, between 20 ng/mL and 50 ng/mL (P<0.01) for bFGF. SCF and bFGF were significantly enhanced mice spermatogonial stem cells proliferation after these three factors combination. For LIF, no obvious effect was abserved.展开更多
Objective： To investigate the efficacy of the locking internal fixator （LIF）, which includes the locking compression plate （LCP） and the less invasive stable system （LISS）, in the proximal and distal tibial fra...Objective： To investigate the efficacy of the locking internal fixator （LIF）, which includes the locking compression plate （LCP） and the less invasive stable system （LISS）, in the proximal and distal tibial fractures. Methods： We did a retrospective study on a total of 98 patients with either proximal or distal tibial fractures from January 2003 to January 2007, who had received the opera- tion with LIF by the minimally invasive plate osteosynthe- sis （MIPO） technique. The data consisted of 43 proximal tibial fractures （type AO41 C3） and 55 distal tibial fractures （type AO43C3）. Results： No complications were observed in all patients after operation. The mean healing time was 8.4 months （range 5-14 months）. Only two cases of delayed union oc- curred at postoperative 10 months. No infections were re- ported after the definitive surgery even in the cases of open fractures. All patients reached a full range of motion at post- operative 6 to 9 months and regained the normal functions of knee and ankle joints. Conclusion： Using LIF in MIPO technique is a reliable approach towards the proximal and distal tibial fractures that are not suitable for intramedullary nailing.展开更多
Lithium metal is supposed to be critical material for constructing next-generation batteries due to extremely high capacity and ultralow redox potential. However, the perplexing issue of lithium dendrite growth impede...Lithium metal is supposed to be critical material for constructing next-generation batteries due to extremely high capacity and ultralow redox potential. However, the perplexing issue of lithium dendrite growth impedes the commercial application. The initial nucleation and low Li ions diffusion rate in the electrolyte/electrode interface dominate the deposition behavior. Therefore, a uniform and flexible interface is urgently needed. Here, a facile method is proposed to prepare a thin and porous LiF-rich layer (TPL) by the in-situ reaction of small amount of ammonium hydrogen difluoride (NH4HF2) and Li metal. The deposition morphology on Li metal anode with LiF layer is significantly flat and homogeneous owning to low lateral diffusion barrier on LiF crystals and the porous structure of TPL film. Additionally, the symmetrical cells made with such TPL Li anodes show significantly stable cycling over 100 cycles at high current density of 6 mA/cm^2. The TPL Li|LiFePO4 full cells keep over 99% capacity retention after 100 cycles at 2.0 C. This approach serves as a facile and controllable way of adjusting the protective layer on Li metal.展开更多
文摘The present study identified the favorable environment conditions for Spermatogomial stem cells in vitro according to their unique biological properties. Three growth factors, stem cell factor (SCF), leukemia inhibitory factor (LIF) and basic fibroblast growth factor (bFGF) were all found to independently contribute to the proliferation of mouse spermatogonial stem cell. The percentage of cell proliferation significantly enhanced by SCF at 30 ng/mL but decreased with heightening its combination after cultured 120 hours. The mice spermatogonial stem cells were significantly proliferated after 120 hours’ culture with 10 ng/mL and 20 ng/mL (P<0.01) of LIF, between 20 ng/mL and 50 ng/mL (P<0.01) for bFGF. SCF and bFGF were significantly enhanced mice spermatogonial stem cells proliferation after these three factors combination. For LIF, no obvious effect was abserved.
文摘Objective： To investigate the efficacy of the locking internal fixator （LIF）, which includes the locking compression plate （LCP） and the less invasive stable system （LISS）, in the proximal and distal tibial fractures. Methods： We did a retrospective study on a total of 98 patients with either proximal or distal tibial fractures from January 2003 to January 2007, who had received the opera- tion with LIF by the minimally invasive plate osteosynthe- sis （MIPO） technique. The data consisted of 43 proximal tibial fractures （type AO41 C3） and 55 distal tibial fractures （type AO43C3）. Results： No complications were observed in all patients after operation. The mean healing time was 8.4 months （range 5-14 months）. Only two cases of delayed union oc- curred at postoperative 10 months. No infections were re- ported after the definitive surgery even in the cases of open fractures. All patients reached a full range of motion at post- operative 6 to 9 months and regained the normal functions of knee and ankle joints. Conclusion： Using LIF in MIPO technique is a reliable approach towards the proximal and distal tibial fractures that are not suitable for intramedullary nailing.
基金supported by the National Basic Research Program of China (Grant no. 2015CB251100)Beijing Natural Science Foundation (No. L182023)
文摘Lithium metal is supposed to be critical material for constructing next-generation batteries due to extremely high capacity and ultralow redox potential. However, the perplexing issue of lithium dendrite growth impedes the commercial application. The initial nucleation and low Li ions diffusion rate in the electrolyte/electrode interface dominate the deposition behavior. Therefore, a uniform and flexible interface is urgently needed. Here, a facile method is proposed to prepare a thin and porous LiF-rich layer (TPL) by the in-situ reaction of small amount of ammonium hydrogen difluoride (NH4HF2) and Li metal. The deposition morphology on Li metal anode with LiF layer is significantly flat and homogeneous owning to low lateral diffusion barrier on LiF crystals and the porous structure of TPL film. Additionally, the symmetrical cells made with such TPL Li anodes show significantly stable cycling over 100 cycles at high current density of 6 mA/cm^2. The TPL Li|LiFePO4 full cells keep over 99% capacity retention after 100 cycles at 2.0 C. This approach serves as a facile and controllable way of adjusting the protective layer on Li metal.
