The second internal transcribed spacer (ITS-2) of Leucocytozoon caulleryi was amplified by PCR using a pair of conserved primers and cloned into the T-T windows of plasmid pGEM-T easy vector. The inserts were successf...The second internal transcribed spacer (ITS-2) of Leucocytozoon caulleryi was amplified by PCR using a pair of conserved primers and cloned into the T-T windows of plasmid pGEM-T easy vector. The inserts were successfully sequenced and the results revealed that the ITS-2 plus flanking sequence (ITS2+) was composed of 270 nucleotides. and the ITS-2 was 113 bp in length. The ITS-2 of L. caulleryi was analysed by NCBI Blast, and the degree of homology of the ITS-2 of L. caulleryi was compared with that of Eimeria tenella , Candida tropicalis and Saccharomyces kluyveri and others by wDNASIS. The results showed that the ITS-2 of L. caulleryi is characteristic and has the greatest similarity with E. tenella (21.2%).展开更多
Leucocytozoonosis was found in three layer farms in chickens with suspected fatty liver or fatty liver hemorrhagic syndrome in Korea between 2009 and 2011.These layer chicken flocks showed both mortality and decreased...Leucocytozoonosis was found in three layer farms in chickens with suspected fatty liver or fatty liver hemorrhagic syndrome in Korea between 2009 and 2011.These layer chicken flocks showed both mortality and decreased egg production for one or two weeks when they were between 59 and 82 weeks old.At the necropsy,the most prominent gross lesions were found in the liver,which was enlarged,had a fragile texture,exhibited yellowish discolorations,and had various hemorrhagic lesions.Tissue reactions associated with megaloschizonts specific for Leucocytozoon caulleryi were prominent upon microscopic examination of the liver without significant lipidosis.In addition,the ovaries and uterus were the most affected organs for Leucocytozoon caulleryi multiplication,which led to decreased egg productions.Molecular studies with formalin-fixed,paraffin-embedded tissues were performed in search of a partial region of the cytochrome b gene for hemosporidian parasites.Based on these results,the causal agent was determined to be closely related to Leucocytozoon caulleryi reported in Japan and Malaysia.In this study,we describe recently re-occurring leucocytozoonosis in layer chickens,which required histopathology for disease diagnosis.To prevent outbreaks and maintain chicken health and egg production,layer chickens need to be monitored for symptoms of leucocytozoonosis.展开更多
基金supported by grants from National Natural Science Foundation of China(39870549)Natural Science Foundation of Guangdong Province(980134).
文摘The second internal transcribed spacer (ITS-2) of Leucocytozoon caulleryi was amplified by PCR using a pair of conserved primers and cloned into the T-T windows of plasmid pGEM-T easy vector. The inserts were successfully sequenced and the results revealed that the ITS-2 plus flanking sequence (ITS2+) was composed of 270 nucleotides. and the ITS-2 was 113 bp in length. The ITS-2 of L. caulleryi was analysed by NCBI Blast, and the degree of homology of the ITS-2 of L. caulleryi was compared with that of Eimeria tenella , Candida tropicalis and Saccharomyces kluyveri and others by wDNASIS. The results showed that the ITS-2 of L. caulleryi is characteristic and has the greatest similarity with E. tenella (21.2%).
基金provided by the project of National Institute of Environment Research(No.2015080777)
文摘Leucocytozoonosis was found in three layer farms in chickens with suspected fatty liver or fatty liver hemorrhagic syndrome in Korea between 2009 and 2011.These layer chicken flocks showed both mortality and decreased egg production for one or two weeks when they were between 59 and 82 weeks old.At the necropsy,the most prominent gross lesions were found in the liver,which was enlarged,had a fragile texture,exhibited yellowish discolorations,and had various hemorrhagic lesions.Tissue reactions associated with megaloschizonts specific for Leucocytozoon caulleryi were prominent upon microscopic examination of the liver without significant lipidosis.In addition,the ovaries and uterus were the most affected organs for Leucocytozoon caulleryi multiplication,which led to decreased egg productions.Molecular studies with formalin-fixed,paraffin-embedded tissues were performed in search of a partial region of the cytochrome b gene for hemosporidian parasites.Based on these results,the causal agent was determined to be closely related to Leucocytozoon caulleryi reported in Japan and Malaysia.In this study,we describe recently re-occurring leucocytozoonosis in layer chickens,which required histopathology for disease diagnosis.To prevent outbreaks and maintain chicken health and egg production,layer chickens need to be monitored for symptoms of leucocytozoonosis.
